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- W2553887793 abstract "The leakage of bacteria through endodontic repair materials is a matter of great concern in dentistry, as the success of repair of furcation perforations or root ends lies in the seal provided by the material against bacterial infiltrations. In vitro microleakage techniques (like dye penetration and fluid filtration) are used to assess the sealing ability of repair materials. However, such microleakage results cannot be correlated with migration of microbes in real situations. The quest for obtaining clinically relevant data prompted researches to develop bacterial leakage models. Various custom set ups had been developed for the purpose. We report a new model for studying bacterial leakage through repair materials, which could be applied universally for teeth of any size and shape. It consists of a cast of the repaired tooth in poly methyl methacrylate (PMMA), incorporated in microbiological test set up. The cast is modified to get a delivery chamber in the upper portion and the lower portion is appropriately sized to allow the repaired part come in contact with the culture medium. The inoculum is delivered to the upper chamber and the leakage of the bacteria is identified through the colony formation in the medium. This model is close to clinical condition and provides reliable and repeatable results. Minimum error parameters, ease of working and reliability are additional features. I. Introduction The leakage of bacteria through endodontic repair materials is a matter of great concern in dentistry. The success of repair of furcation perforations or root ends lies in the seal provided by the material against the oral flora. A bacterial infiltration into the supporting area leads to periodontal breakdown. The sealing ability of the endodontic repair materials is, therefore, an important factor in the clinical success of endodontic procedures 1 . A considerable volume of study is available on this aspect of endodontics, with exhaustive reviews 2-5 . As there are practical difficulties in assessing the bacterial leakage clinically, in vitro microleakage methods are used for the initial evaluation and comparison of the sealing ability of various repair materials. The concept of microleakage relates to the penetration of oral fluids and microorganisms along the material cavity/wall interface following insertion of a restoration. Microspaces are likely at this interface, caused partly by the contraction of the mass of the material during the setting process and partly by the lack of adherence to the cavity walls. Measuring the microleakage could be used to predict the performance of the material in the oral environment 5 . A variety of penetration and filtration techniques has been developed to determine the microleakage through dental restorations, like dye penetration, radioisotope tracing, electrochemical migration and fluid filtration" @default.
- W2553887793 created "2016-11-30" @default.
- W2553887793 date "2013-01-01" @default.
- W2553887793 modified "2023-10-04" @default.
- W2553887793 title "An In Vitro Model for Evaluating Bacterial Leakage Through Endodontic Repair Materials" @default.
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- W2553887793 doi "https://doi.org/10.9790/0853-1144852" @default.
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