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- W2554200484 abstract "Bilirubin is routinely measured in neonates to avoid the irreversible effects of kernicterus. Grossly hemolyzed samples are routinely rejected under the assumption that bilirubin cannot be accurately measured in hemolyzed samples. The objective of this study was to determine the effect of hemolysis on bilirubin measurement in neonates.Data included 2-years of paired bilirubin results (n=70) where hemolyzed and non-hemolyzed samples were collected and measured within 6h of each other. Bilirubin results were compared by Passing-Bablock linear regression and difference plots. Bilirubin results were also compared using the Bhutani nomogram to determine if hemolysis affected the hyperbilirubinemia risk-category.Gross hemolysis resulted in a mean negative bias of -5.2μmol/L (95% CI: -30.7 to 20.3μmol/L). Based on the Bhutani nomogram, 1/70 samples would have been classified as Low-intermediate instead of High-intermediate risk and 3/70 would have been classified as Low instead of Low-intermediate risk; 5/70 samples would have been classified as Low-intermediate instead of Low risk.Collectively the data support that neonatal bilirubin may be reported from grossly hemolyzed samples measured using the Reichert Unistat Bilirubinometer. This practice has been adopted at our institution where results are reported with comments describing the effects of hemolysis. Implementation of this approach has decreased the number of blood re-collections in neonates." @default.
- W2554200484 created "2016-11-30" @default.
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- W2554200484 date "2017-03-01" @default.
- W2554200484 modified "2023-09-23" @default.
- W2554200484 title "Robustness of the Reichert Unistat Bilirubinometer for analysis of hemolyzed samples from neonates" @default.
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- W2554200484 doi "https://doi.org/10.1016/j.clinbiochem.2016.11.012" @default.
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