FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2554219085> ?p ?o ?g. }

• W2554219085 abstract "ABSTRACT Teams of myosin motors carry out intracellular transport and contract the actin cytoskeleton. To fully understand the behavior of multi-myosin ensembles we need to know the properties of individual myosins and the mode of interaction between them. Current models of the interactions within the myosin complex treat the actin filament as a stiff rod, not contributing to the regulation of collective myosin dynamics. Here, we present data suggesting that force transduction through the actin filament is an important element of interaction within myosin-6 ensembles in vitro . Multiple myosin-6s coordinate their steps if they are separated by a short (and therefore high-force bearing) segment of actin. The measurements were performed using Fluorescence Interference Contrast Microscopy (FLIC) to measure small changes in the height of fluorescently labeled actin. Using FLIC, we assign the positions of myosins in a gliding filament assay geometry and measure their attachment time to actin. We also identify actin segments that are buckled or under tension. We show that myosin-6 holds actin about 10 nm above the surface. However, due to asynchronous myosin stepping, frequent buckles up to about 60 nm high appear. The buckle lifetime decreases as the distance between the myosin-6s is reduced, a sign of inter-motor coordination. Our data are consistent with coordinated stepping of closely spaced myosins, but uncoordinated motility with widely separated myosins where buckles can form. These features would be expected to operate on myosins in the cell, where motor spacing may vary considerably depending on the target organelle. SIGNIFICANCE STATEMENT Myosins are molecular motors that carry out intracellular transport. Interactions between the myosins are crucial for understanding their function. Using Fluorescence Interference Contrast (FLIC) microscopy we characterized the interaction between multiple myosin-6 motors immobilized to the surface of a slide and pulling the same actin filament. Our results point towards coordination of myosin steps as a mechanism governing the behavior of a multi-myosin complex. We also demonstrated the unique application of FLIC microscopy for highly parallel identification and measurement of single myosin motors in a gliding filament format. These features of FLIC enable a robust study of collective myosin dynamics." @default.
• W2554219085 created "2016-11-30" @default.
• W2554219085 creator A5011354115 @default.
• W2554219085 creator A5027330546 @default.
• W2554219085 creator A5077104067 @default.
• W2554219085 date "2016-08-06" @default.
• W2554219085 modified "2023-10-16" @default.
• W2554219085 title "A FLIC motility assay reveals myosin-6 coordination limited by actin filament buckling" @default.
• W2554219085 cites W1501204944 @default.
• W2554219085 cites W1520255714 @default.
• W2554219085 cites W1587940648 @default.
• W2554219085 cites W1632862232 @default.
• W2554219085 cites W1976823045 @default.
• W2554219085 cites W1981068779 @default.
• W2554219085 cites W1983572701 @default.
• W2554219085 cites W1984769051 @default.
• W2554219085 cites W1989200068 @default.
• W2554219085 cites W1990381237 @default.
• W2554219085 cites W2003755335 @default.
• W2554219085 cites W2010013914 @default.
• W2554219085 cites W2010897036 @default.
• W2554219085 cites W2017017186 @default.
• W2554219085 cites W2019559746 @default.
• W2554219085 cites W2034477007 @default.
• W2554219085 cites W2047519015 @default.
• W2554219085 cites W2065027762 @default.
• W2554219085 cites W2068996331 @default.
• W2554219085 cites W2070602462 @default.
• W2554219085 cites W2070967303 @default.
• W2554219085 cites W2077482727 @default.
• W2554219085 cites W2078463512 @default.
• W2554219085 cites W2081430724 @default.
• W2554219085 cites W2082834127 @default.
• W2554219085 cites W2089623748 @default.
• W2554219085 cites W2093025565 @default.
• W2554219085 cites W2095633207 @default.
• W2554219085 cites W2108368079 @default.
• W2554219085 cites W2110829617 @default.
• W2554219085 cites W2118855111 @default.
• W2554219085 cites W2126065907 @default.
• W2554219085 cites W2130379504 @default.
• W2554219085 cites W2155418451 @default.
• W2554219085 cites W4251846070 @default.
• W2554219085 cites W952792670 @default.
• W2554219085 cites W2043064410 @default.
• W2554219085 doi "https://doi.org/10.1101/068163" @default.
• W2554219085 hasPublicationYear "2016" @default.
• W2554219085 type Work @default.
• W2554219085 sameAs 2554219085 @default.
• W2554219085 citedByCount "2" @default.
• W2554219085 countsByYear W25542190852019 @default.
• W2554219085 countsByYear W25542190852022 @default.
• W2554219085 crossrefType "posted-content" @default.
• W2554219085 hasAuthorship W2554219085A5011354115 @default.
• W2554219085 hasAuthorship W2554219085A5027330546 @default.
• W2554219085 hasAuthorship W2554219085A5077104067 @default.
• W2554219085 hasBestOaLocation W25542190851 @default.
• W2554219085 hasConcept C12554922 @default.
• W2554219085 hasConcept C125705527 @default.
• W2554219085 hasConcept C14228908 @default.
• W2554219085 hasConcept C142669718 @default.
• W2554219085 hasConcept C1491633281 @default.
• W2554219085 hasConcept C160408235 @default.
• W2554219085 hasConcept C185592680 @default.
• W2554219085 hasConcept C189040839 @default.
• W2554219085 hasConcept C20418707 @default.
• W2554219085 hasConcept C2993400109 @default.
• W2554219085 hasConcept C3620293 @default.
• W2554219085 hasConcept C55493867 @default.
• W2554219085 hasConcept C6997183 @default.
• W2554219085 hasConcept C86803240 @default.
• W2554219085 hasConcept C93126451 @default.
• W2554219085 hasConcept C95444343 @default.
• W2554219085 hasConceptScore W2554219085C12554922 @default.
• W2554219085 hasConceptScore W2554219085C125705527 @default.
• W2554219085 hasConceptScore W2554219085C14228908 @default.
• W2554219085 hasConceptScore W2554219085C142669718 @default.
• W2554219085 hasConceptScore W2554219085C1491633281 @default.
• W2554219085 hasConceptScore W2554219085C160408235 @default.
• W2554219085 hasConceptScore W2554219085C185592680 @default.
• W2554219085 hasConceptScore W2554219085C189040839 @default.
• W2554219085 hasConceptScore W2554219085C20418707 @default.
• W2554219085 hasConceptScore W2554219085C2993400109 @default.
• W2554219085 hasConceptScore W2554219085C3620293 @default.
• W2554219085 hasConceptScore W2554219085C55493867 @default.
• W2554219085 hasConceptScore W2554219085C6997183 @default.
• W2554219085 hasConceptScore W2554219085C86803240 @default.
• W2554219085 hasConceptScore W2554219085C93126451 @default.
• W2554219085 hasConceptScore W2554219085C95444343 @default.
• W2554219085 hasLocation W25542190851 @default.
• W2554219085 hasOpenAccess W2554219085 @default.
• W2554219085 hasPrimaryLocation W25542190851 @default.
• W2554219085 hasRelatedWork W1632862232 @default.
• W2554219085 hasRelatedWork W2025863089 @default.
• W2554219085 hasRelatedWork W2075538444 @default.
• W2554219085 hasRelatedWork W2089002974 @default.
• W2554219085 hasRelatedWork W2108187594 @default.
• W2554219085 hasRelatedWork W2155131980 @default.
• W2554219085 hasRelatedWork W2554219085 @default.
• W2554219085 hasRelatedWork W2738108552 @default.

• next