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- W2554372608 abstract "Molecular inversion probes (MIPs) in combination with massively parallel DNA sequencing represent a versatile, yet economical tool for targeted sequencing of genomic DNA. Several thousand genomic targets can be selectively captured using long oligonucleotides containing unique targeting arms and universal linkers. The ability to append sequencing adaptors and sample-specific barcodes allows large-scale pooling and subsequent high-throughput sequencing at relatively low cost per sample. Here, we describe a wet bench protocol detailing the capture and subsequent sequencing of >2000 genomic targets from 192 samples, representative of a single lane on the Illumina HiSeq 2000 platform." @default.
- W2554372608 created "2016-11-30" @default.
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- W2554372608 date "2016-11-08" @default.
- W2554372608 modified "2023-09-23" @default.
- W2554372608 title "Targeted Capture and High-Throughput Sequencing Using Molecular Inversion Probes (MIPs)" @default.
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- W2554372608 doi "https://doi.org/10.1007/978-1-4939-6442-0_6" @default.
- W2554372608 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5484527" @default.
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