Matches in SemOpenAlex for { <https://semopenalex.org/work/W2554488016> ?p ?o ?g. }
- W2554488016 abstract "Proteins of the transforming-growth-factor-β (TGF-β)-superfamily have a remarkable ability to induce cartilage and bone and the crosstalk of TGF-β - and BMP-signalling pathways appears crucial during chondrocyte development. Aim was to assess the regulation of TGF-β-superfamily members and of Smad2/3- and Smad1/5/9-signalling during endochondral in vitro chondrogenesis of mesenchymal stromal cells (MSC) relative to chondral redifferentiation of articular chondrocytes (AC) to adjust chondrocyte development of MSC towards a less hypertrophic phenotype. While MSC increased BMP4 and BMP7 and reduced TGFBR2 and TGFBR3-expression during chondrogenesis, an opposite regulation was observed during AC-redifferentiation. Antagonists CHRD and CHL2 rose significantly only in AC-cultures. AC showed higher initial BMP4, pSmad1/5/9 and SOX9 protein levels, a faster (re-)differentiation but a similar decline of pSmad2/3- and pSmad1/5/9-signalling versus MSC-cultures. BMP-4/7-stimulation of MSC-pellets enhanced SOX9 and accelerated ALP-induction but did not shift differentiation towards osteogenesis. Inhibition of BMP-signalling by dorsomorphin significantly reduced SOX9, raised RUNX2, maintained collagen-type-II and collagen-type-X lower and kept ALP-activity at levels reached at initiation of treatment. Conclusively, ALK1,2,3,6-signalling was essential for MSC-chondrogenesis and its prochondrogenic rather than prohypertrophic role may explain why inhibition of canonical BMP-signalling could not uncouple cartilage matrix production from hypertrophy as this was achieved with pulsed PTHrP-application." @default.
- W2554488016 created "2016-11-30" @default.
- W2554488016 creator A5016326216 @default.
- W2554488016 creator A5025637377 @default.
- W2554488016 creator A5028355843 @default.
- W2554488016 creator A5043030734 @default.
- W2554488016 creator A5061312686 @default.
- W2554488016 creator A5065713011 @default.
- W2554488016 date "2016-11-16" @default.
- W2554488016 modified "2023-10-01" @default.
- W2554488016 title "Differential expression of TGF-β superfamily members and role of Smad1/5/9-signalling in chondral versus endochondral chondrocyte differentiation" @default.
- W2554488016 cites W1175185197 @default.
- W2554488016 cites W1515190166 @default.
- W2554488016 cites W1677560495 @default.
- W2554488016 cites W1965073410 @default.
- W2554488016 cites W1967269017 @default.
- W2554488016 cites W1972249277 @default.
- W2554488016 cites W1972979483 @default.
- W2554488016 cites W1976808417 @default.
- W2554488016 cites W1986469118 @default.
- W2554488016 cites W1989739945 @default.
- W2554488016 cites W1993381081 @default.
- W2554488016 cites W1993567370 @default.
- W2554488016 cites W1995944358 @default.
- W2554488016 cites W1999319535 @default.
- W2554488016 cites W2011576192 @default.
- W2554488016 cites W2015050458 @default.
- W2554488016 cites W2025831725 @default.
- W2554488016 cites W2026720122 @default.
- W2554488016 cites W2034115851 @default.
- W2554488016 cites W2042932723 @default.
- W2554488016 cites W2047642636 @default.
- W2554488016 cites W2048414529 @default.
- W2554488016 cites W2049751449 @default.
- W2554488016 cites W2058104849 @default.
- W2554488016 cites W2060299179 @default.
- W2554488016 cites W2067716147 @default.
- W2554488016 cites W2071788329 @default.
- W2554488016 cites W2073152395 @default.
- W2554488016 cites W2073397316 @default.
- W2554488016 cites W2077309660 @default.
- W2554488016 cites W2080242404 @default.
- W2554488016 cites W2087174236 @default.
- W2554488016 cites W2087370958 @default.
- W2554488016 cites W2098183783 @default.
- W2554488016 cites W2101469591 @default.
- W2554488016 cites W2104283759 @default.
- W2554488016 cites W2110654810 @default.
- W2554488016 cites W2122433264 @default.
- W2554488016 cites W2122683221 @default.
- W2554488016 cites W2123148021 @default.
- W2554488016 cites W2123819897 @default.
- W2554488016 cites W2132803736 @default.
- W2554488016 cites W2135930613 @default.
- W2554488016 cites W2140077756 @default.
- W2554488016 cites W2151840099 @default.
- W2554488016 cites W2179071860 @default.
- W2554488016 cites W2200360423 @default.
- W2554488016 cites W2294976869 @default.
- W2554488016 cites W2302693978 @default.
- W2554488016 doi "https://doi.org/10.1038/srep36655" @default.
- W2554488016 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5111074" @default.
- W2554488016 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27848974" @default.
- W2554488016 hasPublicationYear "2016" @default.
- W2554488016 type Work @default.
- W2554488016 sameAs 2554488016 @default.
- W2554488016 citedByCount "64" @default.
- W2554488016 countsByYear W25544880162017 @default.
- W2554488016 countsByYear W25544880162018 @default.
- W2554488016 countsByYear W25544880162019 @default.
- W2554488016 countsByYear W25544880162020 @default.
- W2554488016 countsByYear W25544880162021 @default.
- W2554488016 countsByYear W25544880162022 @default.
- W2554488016 countsByYear W25544880162023 @default.
- W2554488016 crossrefType "journal-article" @default.
- W2554488016 hasAuthorship W2554488016A5016326216 @default.
- W2554488016 hasAuthorship W2554488016A5025637377 @default.
- W2554488016 hasAuthorship W2554488016A5028355843 @default.
- W2554488016 hasAuthorship W2554488016A5043030734 @default.
- W2554488016 hasAuthorship W2554488016A5061312686 @default.
- W2554488016 hasAuthorship W2554488016A5065713011 @default.
- W2554488016 hasBestOaLocation W25544880161 @default.
- W2554488016 hasConcept C104317684 @default.
- W2554488016 hasConcept C105702510 @default.
- W2554488016 hasConcept C118131993 @default.
- W2554488016 hasConcept C146539212 @default.
- W2554488016 hasConcept C150194340 @default.
- W2554488016 hasConcept C17938293 @default.
- W2554488016 hasConcept C185592680 @default.
- W2554488016 hasConcept C198826908 @default.
- W2554488016 hasConcept C202751555 @default.
- W2554488016 hasConcept C2776363554 @default.
- W2554488016 hasConcept C2778260815 @default.
- W2554488016 hasConcept C2778486617 @default.
- W2554488016 hasConcept C2780550940 @default.
- W2554488016 hasConcept C2781013607 @default.
- W2554488016 hasConcept C2781403057 @default.
- W2554488016 hasConcept C30352789 @default.
- W2554488016 hasConcept C31507581 @default.
- W2554488016 hasConcept C35496256 @default.