FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2554926479> ?p ?o ?g. }

• W2554926479 endingPage "33070" @default.
• W2554926479 startingPage "33070" @default.
• W2554926479 abstract "BackgroundAggressive periodontitis is associated with the presence of Aggregatibacter actinomycetemcomitans, a leukotoxin (Ltx)-producing periodontal pathogen. Ltx has the ability to lyse white blood cells including neutrophils.ObjectivesThis study was aimed at investigating the interactions between neutrophils and Ltx with regard to the chemotactic properties of Ltx and the release of neutrophil extracellular traps (NETs).MethodsNeutrophils from healthy blood donors were isolated and incubated for 30 min and 3 h with increasing concentrations of Ltx (1, 10, and 100 ng/mL) as well as with A. actinomycetemcomitans strains (NCTC 9710 and HK 1651) producing different levels of Ltx. Formation of NETs and cell lysis were assessed by microscopy, fluorescence-based assays, and measurement of released lactate dehydrogenase. Neutrophil migration in response to different Ltx gradients was monitored by real-time video microscopy, and image analysis was performed using ImageJ software.ResultsAlthough Ltx (10 and 100 ng/mL) and the leukotoxic A. actinomycetemcomitans strain HK 1651 lysed some neutrophils, other cells were still capable of performing NETosis in a concentration-dependent manner. Low doses of Ltx and the weakly leukotoxic strain NCTC 9710 did not lead to neutrophil lysis, but did induce some NETosis. Furthermore, all three concentrations of Ltx enhanced random neutrophil movement; however, low directional accuracy was observed compared with the positive control (fMLP).ConclusionsThe results indicate that Ltx acts both as a neutrophil activator and also causes cell death. In addition, Ltx directly induces NETosis in neutrophils prior to cell lysis. In future studies, the underlying pathways involved in Ltx-meditated neutrophil activation and NETosis need to be investigated further." @default.
• W2554926479 created "2016-11-30" @default.
• W2554926479 creator A5012697008 @default.
• W2554926479 creator A5033185814 @default.
• W2554926479 creator A5045609820 @default.
• W2554926479 creator A5060236774 @default.
• W2554926479 creator A5063557940 @default.
• W2554926479 creator A5070092970 @default.
• W2554926479 creator A5088201442 @default.
• W2554926479 date "2016-01-01" @default.
• W2554926479 modified "2023-10-10" @default.
• W2554926479 title "Effects of<i>Aggregatibacter actinomycetemcomitans</i>leukotoxin on neutrophil migration and extracellular trap formation" @default.
• W2554926479 cites W1598588804 @default.
• W2554926479 cites W1600560134 @default.
• W2554926479 cites W1675847437 @default.
• W2554926479 cites W1830252659 @default.
• W2554926479 cites W1844472299 @default.
• W2554926479 cites W1914201051 @default.
• W2554926479 cites W1969852564 @default.
• W2554926479 cites W1971362950 @default.
• W2554926479 cites W1977373426 @default.
• W2554926479 cites W1977550761 @default.
• W2554926479 cites W1982268827 @default.
• W2554926479 cites W1987756444 @default.
• W2554926479 cites W1991021491 @default.
• W2554926479 cites W1997048268 @default.
• W2554926479 cites W1997981355 @default.
• W2554926479 cites W1999292038 @default.
• W2554926479 cites W2002070061 @default.
• W2554926479 cites W2003265848 @default.
• W2554926479 cites W2004399489 @default.
• W2554926479 cites W2015145838 @default.
• W2554926479 cites W2016918716 @default.
• W2554926479 cites W2018860370 @default.
• W2554926479 cites W2031474938 @default.
• W2554926479 cites W2039789500 @default.
• W2554926479 cites W2041897764 @default.
• W2554926479 cites W2065696203 @default.
• W2554926479 cites W2071831285 @default.
• W2554926479 cites W2074108759 @default.
• W2554926479 cites W2082367745 @default.
• W2554926479 cites W2091916302 @default.
• W2554926479 cites W2096068443 @default.
• W2554926479 cites W2098610918 @default.
• W2554926479 cites W2104934425 @default.
• W2554926479 cites W2105493788 @default.
• W2554926479 cites W2106259459 @default.
• W2554926479 cites W2107726923 @default.
• W2554926479 cites W2109307543 @default.
• W2554926479 cites W2125045860 @default.
• W2554926479 cites W2130280651 @default.
• W2554926479 cites W2137200078 @default.
• W2554926479 cites W2137625680 @default.
• W2554926479 cites W2137878745 @default.
• W2554926479 cites W2139670223 @default.
• W2554926479 cites W2145730621 @default.
• W2554926479 cites W2151444835 @default.
• W2554926479 cites W2158887211 @default.
• W2554926479 cites W2171105146 @default.
• W2554926479 cites W2171714447 @default.
• W2554926479 cites W2226774535 @default.
• W2554926479 cites W2297386413 @default.
• W2554926479 cites W2518611640 @default.
• W2554926479 cites W4256138746 @default.
• W2554926479 cites W4312369685 @default.
• W2554926479 doi "https://doi.org/10.3402/jom.v8.33070" @default.
• W2554926479 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5103672" @default.
• W2554926479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27834173" @default.
• W2554926479 hasPublicationYear "2016" @default.
• W2554926479 type Work @default.
• W2554926479 sameAs 2554926479 @default.
• W2554926479 citedByCount "32" @default.
• W2554926479 countsByYear W25549264792017 @default.
• W2554926479 countsByYear W25549264792018 @default.
• W2554926479 countsByYear W25549264792019 @default.
• W2554926479 countsByYear W25549264792020 @default.
• W2554926479 countsByYear W25549264792021 @default.
• W2554926479 countsByYear W25549264792022 @default.
• W2554926479 countsByYear W25549264792023 @default.
• W2554926479 crossrefType "journal-article" @default.
• W2554926479 hasAuthorship W2554926479A5012697008 @default.
• W2554926479 hasAuthorship W2554926479A5033185814 @default.
• W2554926479 hasAuthorship W2554926479A5045609820 @default.
• W2554926479 hasAuthorship W2554926479A5060236774 @default.
• W2554926479 hasAuthorship W2554926479A5063557940 @default.
• W2554926479 hasAuthorship W2554926479A5070092970 @default.
• W2554926479 hasAuthorship W2554926479A5088201442 @default.
• W2554926479 hasBestOaLocation W25549264791 @default.
• W2554926479 hasConcept C126322002 @default.
• W2554926479 hasConcept C134897094 @default.
• W2554926479 hasConcept C170493617 @default.
• W2554926479 hasConcept C185592680 @default.
• W2554926479 hasConcept C198677920 @default.
• W2554926479 hasConcept C203014093 @default.
• W2554926479 hasConcept C2776914184 @default.
• W2554926479 hasConcept C2778714489 @default.
• W2554926479 hasConcept C2780183776 @default.
• W2554926479 hasConcept C2780385504 @default.

• next