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• W2554989798 abstract "The interaction of Pseudomonas syringae with Arabidopsis is one of the most commonly used systems to study various bacterial—host interrelationships. Currently, most studies are based on the growth quantification of the pathogen to characterize resistance or virulence targets. However, the standard available method for determining bacterial proliferation in planta is laborious and has several limitations. Here we present an alternative robust approach, which is based on the quantification of bacterial DNA by real-time PCR. We directly compared this assay with the routinely used plate counting method to access bacterial titers in a number of well described Arabidopsis mutants. These studies showed that the DNA-based technique is highly reliable and comparable. Moreover, the technique is easily applicable, robust, and ideal for routine experiments or for larger scale analyses." @default.
• W2554989798 created "2016-11-30" @default.
• W2554989798 creator A5033223714 @default.
• W2554989798 creator A5044780272 @default.
• W2554989798 date "2016-11-21" @default.
• W2554989798 modified "2023-10-02" @default.
• W2554989798 title "A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogen Pseudomonas syringae on Arabidopsis thaliana" @default.
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• W2554989798 doi "https://doi.org/10.1186/s13007-016-0149-z" @default.
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