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- W2555145269 abstract "Fucooligosaccharide is one of the major active components in brown algae. Fucooligosaccharide (FOS) possesses antioxidative, anti-coagulation and antiviral effects, but its intestinal bioavailability needs further evaluation. This study aims to produce FOS from S. siliquosum and to determine their absorption and transportation in human enterocytes. Exploring the absorption and transportation of the Fuc-S1 and Fuc-S2 mixture by using the in vitro Caco-2 monolayers confirmed that the concentration of Fuc-S1 in the apical fluid decreased from 3.71 ± 0.73 mg/ml to 3.04 ± 0.34 mg/ml, whereas the concentration in the basolateral fluid increased from 2.33 ± 0.31 mg/ml to 2.80 ± 0.53 mg/ml. Therefore, the amount of Fuc-S1 absorbed by the Caco-2 monolayers was approximately 4.07 ± 0.60 mg/ml. Furthermore, the concentration of Fuc-S2 in the upper layer solution decreased from 2.93 ± 0.24 mg/ml to 2.14 ± 0.86 mg/ml, whereas the concentration in the lower layer solution increased from 2.15 ± 0.18 mg/ml to 3.10 ± 0.27 mg/ml. Accordingly, the amount of Fuc-S2 absorbed by the Caco-2 monolayers was approximately 4.48 ± 0.80 mg/ml. Loading Fuc-S1 and Fuc-S2 into an intestinal Caco-2 system to test the associated reactions for 90 min revealed that the transportation and cellularabsorption rates of Fuc-S1 were 28.1% and 39.0%, respectively, whereas those of Fuc-S2 were 31% and 47.6%, respectively." @default.
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- W2555145269 date "2015-03-01" @default.
- W2555145269 modified "2023-09-28" @default.
- W2555145269 title "Enzymes-hydrolyzed Fucooligosaccharides from Sargassum siliquosum and Their Intestinal Transportation Across Human Caco-2 Cells" @default.
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