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• W2555333487 abstract "Fungal DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides airborne, handling and cross-contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of materials and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction, and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the diagnosis of fungal organisms at the limit of detection." @default.
• W2555333487 created "2016-11-30" @default.
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• W2555333487 date "2016-11-12" @default.
• W2555333487 modified "2023-09-25" @default.
• W2555333487 title "Fungal-Grade Reagents and Materials for Molecular Analysis" @default.
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• W2555333487 doi "https://doi.org/10.1007/978-1-4939-6515-1_6" @default.
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