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- W2555343121 abstract "Abstract Motivation The past decade has seen an exponential increase in biological sequencing capacity, and there has been a simultaneous effort to help organize and archive some of the vast quantities of sequencing data that are being generated. While these developments are tremendous from the perspective of maximizing the scientific utility of available data, they come with heavy costs. The storage and transmission of such vast amounts of sequencing data is expensive. Results We present Quark , a semi-reference-based compression tool designed for RNA-seq data. Quark makes use of a reference sequence when encoding reads, but produces a representation that can be decoded independently, without the need for a reference. This allows Quark to achieve markedly better compression rates than existing reference-free schemes, while still relieving the burden of assuming a specific, shared reference sequence between the encoder and decoder. We demonstrate that Quark achieves state-of-the-art compression rates, and that, typically, only a small fraction of the reference sequence must be encoded along with the reads to allow reference-free decompression. Availability Quark is implemented in C++11 , and is available under a GPLv3 license at www.github.com/COMBINE-lab/quark . Contact rob.patro@cs.stonybrook.edu" @default.
- W2555343121 created "2016-11-30" @default.
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- W2555343121 date "2016-11-05" @default.
- W2555343121 modified "2023-09-23" @default.
- W2555343121 title "Quark enables semi-reference-based compression of RNA-seq data" @default.
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- W2555343121 doi "https://doi.org/10.1101/085878" @default.
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