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• W2555398315 abstract "This unit details the use of transient expression in mammalian cells to screen cDNA libraries with monoclonal antibodies (MAb) to isolate cDNA clones encoding cell-surface and intracellular proteins. The first protocol in this unit describes the cloning of cDNAs encoding cell-surface antigens. Several steps in this protocol involve transfection procedures that are described in greater detail elsewhere in this volume. The second protocol is a modification that facilitates isolation of cDNAs encoding antigens that are expressed intracellularly. Both protocols are designed for use with the expression vector CDM8, which contains a polylinker for subcloning double-stranded cDNA." @default.
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• W2555398315 date "2003-04-01" @default.
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• W2555398315 title "Use of Monoclonal Antibodies for Expression Cloning" @default.
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• W2555398315 doi "https://doi.org/10.1002/0471142727.mb0611s62" @default.
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