FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2555558145> ?p ?o ?g. }

• W2555558145 endingPage "4078" @default.
• W2555558145 startingPage "4062" @default.
• W2555558145 abstract "// Xianyong Ma 1 , Jinglan Wang 1 , Jianhui Wang 1 , Charles X. Ma 2 , Xiaobin Gao 1 , Vytas Patriub 1 , Jeffrey L. Sklar 1 1 Department of Pathology, Yale University School of Medicine, New Haven, CT, USA 2 University of Connecticut School of Medicine, Farmington, CT, USA Correspondence to: Xianyong Ma, email: xian-yong.ma@yale.edu Keywords: SUZ12 and PRC2 complex, endometrial stromal Sarcoma (ESS), t(7,17) translocation, histone methyl transferase (HMT), H3K27Me3 Received: September 17, 2015      Accepted: October 29, 2016      Published: November 10, 2016 ABSTRACT The Polycomb repressive complex 2 (PRC2), which contains three core proteins EZH2, EED and SUZ12, controls chromatin compaction and transcription repression through trimethylation of lysine 27 on histone 3. The (7;17)(p15;q21) chromosomal translocation present in most cases of endometrial stromal sarcomas (ESSs) results in the in-frame fusion of the JAZF1 and SUZ12 genes. We have investigated whether and how the fusion protein JAZF1-SUZ12 functionally alters PRC2. We found that the fusion protein exists at high levels in ESS containing the t(7;17). Co-transient transfection assay indicated JAZF1-SUZ12 destabilized PRC2 components EZH2 and EED, resulting in decreased histone methyl transferase (HMT) activity, which was confirmed by in vitro studies using reconstituted PRC2 and nucleosome array substrates. We also demonstrated the PRC2 containing the fusion protein decreased the binding affinity to target chromatin loci. In addition, we found that trimethylation of H3K27 was decreased in ESS samples with the t(7;17), but there was no detectable change in H3K9 in these tissues. Moreover, re-expression of SUZ12 in Suz12 (−/−) ES cells rescued the neuronal differentiation while the fusion protein failed to restore this function and enhanced cell proliferation. In summary, our studies reveal that JAZF1-SUZ12 fusion protein disrupts the PRC2 complex, abolishes HMT activity and subsequently activates chromatin/genes normally repressed by PRC2. Such dyesfunction of PRC2 inhibits normal neural differentiation of ES cell and increases cell proliferation. Related changes induced by the JAZF-SUZ12 protein in endometrial stromal cells may explain the oncogenic effect of the t(7;17) in ESS." @default.
• W2555558145 created "2016-11-30" @default.
• W2555558145 creator A5011279096 @default.
• W2555558145 creator A5012563381 @default.
• W2555558145 creator A5013036854 @default.
• W2555558145 creator A5039926439 @default.
• W2555558145 creator A5044306210 @default.
• W2555558145 creator A5054666288 @default.
• W2555558145 creator A5085704495 @default.
• W2555558145 date "2016-11-10" @default.
• W2555558145 modified "2023-10-01" @default.
• W2555558145 title "The JAZF1-SUZ12 fusion protein disrupts PRC2 complexes and impairs chromatin repression during human endometrial stromal tumorogenesis" @default.
• W2555558145 cites W1537671493 @default.
• W2555558145 cites W1967497885 @default.
• W2555558145 cites W1973796290 @default.
• W2555558145 cites W1974330670 @default.
• W2555558145 cites W1974745874 @default.
• W2555558145 cites W1975093996 @default.
• W2555558145 cites W1975678442 @default.
• W2555558145 cites W1976242511 @default.
• W2555558145 cites W1980537562 @default.
• W2555558145 cites W1985162118 @default.
• W2555558145 cites W1989474213 @default.
• W2555558145 cites W1991914845 @default.
• W2555558145 cites W1993372589 @default.
• W2555558145 cites W1996487270 @default.
• W2555558145 cites W1999349086 @default.
• W2555558145 cites W2003313289 @default.
• W2555558145 cites W2013189329 @default.
• W2555558145 cites W2014198756 @default.
• W2555558145 cites W2014359105 @default.
• W2555558145 cites W2015132841 @default.
• W2555558145 cites W2015346703 @default.
• W2555558145 cites W2015416439 @default.
• W2555558145 cites W2021399061 @default.
• W2555558145 cites W2025421620 @default.
• W2555558145 cites W2025553576 @default.
• W2555558145 cites W2030322070 @default.
• W2555558145 cites W2032499371 @default.
• W2555558145 cites W2037128257 @default.
• W2555558145 cites W2044992835 @default.
• W2555558145 cites W2046345187 @default.
• W2555558145 cites W2058386953 @default.
• W2555558145 cites W2060248486 @default.
• W2555558145 cites W2060870400 @default.
• W2555558145 cites W2066305078 @default.
• W2555558145 cites W2070604281 @default.
• W2555558145 cites W2073157961 @default.
• W2555558145 cites W2079406514 @default.
• W2555558145 cites W2081091627 @default.
• W2555558145 cites W2082959814 @default.
• W2555558145 cites W2083513924 @default.
• W2555558145 cites W2086145199 @default.
• W2555558145 cites W2097166664 @default.
• W2555558145 cites W2100365251 @default.
• W2555558145 cites W2102883893 @default.
• W2555558145 cites W2107301489 @default.
• W2555558145 cites W2108549889 @default.
• W2555558145 cites W2114354078 @default.
• W2555558145 cites W2116235936 @default.
• W2555558145 cites W2119376406 @default.
• W2555558145 cites W2119938322 @default.
• W2555558145 cites W2120060263 @default.
• W2555558145 cites W2122076742 @default.
• W2555558145 cites W2123419812 @default.
• W2555558145 cites W2123731635 @default.
• W2555558145 cites W2124035206 @default.
• W2555558145 cites W2129768923 @default.
• W2555558145 cites W2153667947 @default.
• W2555558145 cites W2155990718 @default.
• W2555558145 cites W2162298614 @default.
• W2555558145 cites W2168534340 @default.
• W2555558145 cites W2171260083 @default.
• W2555558145 cites W2171953865 @default.
• W2555558145 cites W2172559090 @default.
• W2555558145 cites W2181751096 @default.
• W2555558145 cites W2181877403 @default.
• W2555558145 cites W2187478032 @default.
• W2555558145 cites W60404606 @default.
• W2555558145 doi "https://doi.org/10.18632/oncotarget.13270" @default.
• W2555558145 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5354813" @default.
• W2555558145 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27845897" @default.
• W2555558145 hasPublicationYear "2016" @default.
• W2555558145 type Work @default.
• W2555558145 sameAs 2555558145 @default.
• W2555558145 citedByCount "48" @default.
• W2555558145 countsByYear W25555581452017 @default.
• W2555558145 countsByYear W25555581452018 @default.
• W2555558145 countsByYear W25555581452019 @default.
• W2555558145 countsByYear W25555581452020 @default.
• W2555558145 countsByYear W25555581452021 @default.
• W2555558145 countsByYear W25555581452022 @default.
• W2555558145 countsByYear W25555581452023 @default.
• W2555558145 crossrefType "journal-article" @default.
• W2555558145 hasAuthorship W2555558145A5011279096 @default.
• W2555558145 hasAuthorship W2555558145A5012563381 @default.
• W2555558145 hasAuthorship W2555558145A5013036854 @default.
• W2555558145 hasAuthorship W2555558145A5039926439 @default.

• next