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- W2555755762 abstract "Lipomobilization is an important factor during transition period in dairy cattle. An exceeded lipomobilization, as in the lipomobilization syndrome, is leading to typical production diseases like displaced abomasum, ketosis or fatty liver and additionally causes a decreased fertility (GRUMMER 1993; DRACKLEY 1999; LEBLANC 2010; OSPINA et al. 2010; MCART et al. 2012). This has economic consequences for farmers. Therefore it is important to prevent exceeded lipomobilization. Overcondition is known to cause lipomobilization syndrome post partum (ROCHE 2006; ROCHE et al. 2007). Therefore, a precise assessment of the body condition is essential. For humans it is known, that especially to much visceral fat leads to health problems as diabetes and the metabolic syndrome (WAJCHENBERG 2000; LEE et al. 2013). Also in cattle there are studies that show metabolic differences between subcutaneous and visceral adipose tissue, like higher lipolytic activity in retroperitoneal fat tissue (LOCHER et al. 2011; VON SOOSTEN et al. 2011; LOCHER et al. 2012; HAUSSLER et al. 2013; JI et al. 2014; KABARA et al. 2014; SAREMI et al. 2014). Also overfeeding in cattle leads to a faster accumulation in the mesenteric fat depot (DRACKLEY et al. 2014). RIBEIRO et al. 2008 developed a method to assess internal fat (IFAT) in beef cattle by measuring kidney fat depth ultrasonographically. This provides a possibility to detect the dynamic changes of IFAT in one animal over a certain period of time, without slaughtering the animal. Nevertheless only the total amount of fat in an animal is estimated and not single depots. The aim of the first part of this work was to develop a method to assess the weight of different fat depots of German Holstein by using ultrasonographic measuring points and body size measurements. Therefore 29 German Holstein cows and heifers were examined ultrasonographically and slaughtered afterwards. The transcutaneous ultrasonographic measuring points were located to measure subcutaneous fat thickness, thickness of the abdominal wall, kidney fat thickness and retroperitoneal fat thickness over the liver. After slaughter process fat was dissected from the carcass and weight separately as subcutaneous, total abdominal, retroperitoneal, omental and mesenteric fat depot. The previous measurement of the fat thickness was done in duplicate and afterwards means were calculated. Further statistical analysis was done with means of every ultrasonographic measuring point. Statistical analysis was done using SAS 9.3. A stepwise multivariate regression analysis was calculated for the five mentioned fat depots. For calculating the depots all measuring points and body sizes were used. Repeated measurements for two animals on two days and calculation of variation coefficients of each ultrasonographic measuring point were done to check repeatability. Additionally root mean square error (RMSE) was calculated to check the accuracy of the estimation equations. Furthermore estimated and actual values of the fat depots were correlated. All those calculations showed that estimation of the above mentioned fat depots is possible with those ultrasonographic measurements with sufficient precision. This method provides a possibility to examine the dynamic changes of those five fat depots over a certain period of time. In the second part of this study the developed method was used to examine the changes of the mentioned fat depots during the last 42 days before estimated parturition and first 100 days in milk and testing two types of feeding regimes with and without supplementation of niacin. The animals were divided into two basic feeding groups. The low concentrate group (LC, n = 22) got feed with practical orientation, starting at day 42 ap with 30 % concentrate and 70 % roughage, which was increased from day 1 pp until day 16 pp up to 50 % concentrate and 50 % roughage. The high concentrate group (HC, n = 25) started with 60 % concentrate and 40 % roughage, was decreased after calving to 30 % concentrate and 70 % roughage and raised slowly up to 50 % concentrate and 50 % roughage until day 24. Feeding level of 50 % concentrate and 50 % roughage in both groups was maintained until the end of the trial at day 100. Roughage included 50 % corn silage and 50 % grass silage on DM basis and was fed ad libitum via self- feeding stations (type RIC, Insentec B.V., Merknese, The Netherlands) (Tienken, oral communication). The LC and HC group were again subdivided into two groups. One of each as a control group (LCC, n = 12, HCC, n = 13) and one of each with a supplementation of 24 g powdered, not rumen protected NA (Mianyang Vanetta Pharmaceutical Technology Co., Ltd, Sichuan, 119 China) included in 1 kg pelleted concentrate per day between day 42 ap and day 24 pp (LCN, n = 10, HCN, n = 12). Each of the four feeding groups contained heifers and cows. The ultrasonographic measurement took place on day 42 ap, day 3, 21 and 100 pp. To detect the de- and increase of the fat depots differences in three time periods were calculated. Fist the accumulation in the dry period between day 42 ap and 3 pp was calculated, than the decrease in FC between day 3 and 21 and additionally between day 21 and100 pp in EL. Furthermore the daily de- and increase was calculated for the same time periods assuming a linear relationship in those time periods. Estimated absolute weight and calculated differences were subjected to an analysis of variance (ANOVA) using PROC GLM of SAS 9.3 for repeated measurements at four points and three time periods of time with time, feeding regime, niacin, parity and interactions between them as factors. Afterwards an ANOVA with the factors time, adipose tissue depot and the interaction between them was calculated to detect the dynamics between the calculated adipose tissue depots. To examine if the values of NEFA and BHB are related to the adipose tissue parameters a pearsons correlation analysis was accomplished. Feeding did show an effect on the daily mobilization of OMAT of FC. The HCN group had the lowest mobilization in this period. AAT is significantly higher than SCAT at all points of time. Also the absolute mobilization of AAT is higher than SCAT but not the relative. Looking on the subdivisions of AAT in comparison with SCAT, SCAT and OMAT are bigger than MAT and RPAT. No significant distinctions could be detected in the absolute differences. But MAT is mobilizing relatively more in FC than SCAT. The correlation analysis showed dynamics of adipose tissue depots have an impact on NEFA on 35 DIM. NEFA on 35 DIM is negatively correlated with absDpD of AAT in FC. In conclusion we can say that there are differences concerning mobilization of different fat depots in dairy cattle. Adipose tissue in the abdominal region of the cow should be regarded if analyzing body condition and evaluating possible risks for metabolic disorders provoked by enhanced lipomobilization. The presented method provides a possibility to examine dynamic differences in fat depots over certain time periods under experimental conditions." @default.
- W2555755762 created "2016-11-30" @default.
- W2555755762 creator A5048172901 @default.
- W2555755762 date "2015-01-01" @default.
- W2555755762 modified "2023-09-26" @default.
- W2555755762 title "Quantifizierung unterschiedlicher Fettdepots mittels Ultrasonographie und deren Entwicklung in der Transitperiode unter dem Einfluss von Niacin bei Milchkühen" @default.
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