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- W2555772000 abstract "Incubation of primary monolayer cultures of hu- man umbilical vein endothelial cells with buffer, thrombin (0.5 unit/ ml), ionophore A23187.(10 ,uM), arachidonic acid (20 /iM), or pros- taglandin H2 (PGH2) (4 pM) resulted in prostacyclin (PGI2) pro- duction in nanomolar quantities to the extent of 36 ? 2, 276 ? 13, 485 ? 32, 533 ? 22, and 532 ?, 22, respectively, as measured by radioimmunoassay of 6-keto-PGFia. Preincubation of the endo- thelium with 1 mM 8-(N,N-diethylamino)-octyl-3,4,5-trimethoxy- benzoate, an antagonist of cytoplasmic Ca2, or with 4 mM 1- methyl-3-isobutylxanthine (MIX), an inhibitorof cyclic nucleotide phosphodiesterase activity, blocked PGI2 release induced by thrombin or A23187, decreased arachidonic acid-induced release by 50%, but had no effect on PGH2-induced release. Radioim- munoassay of cAMP in the endothelium showed that the basal level (1.85 ? 0.14 pmol of cAMP per 4.5 X 105 cells) was increased by an average of 3.9-fold with 4 mM'MIX. PGI2 (0.4 pM) had no sig- nificant effect on cAMP levels in the absence of MIX, but caused a 2-fold increase with 4 mM MIX. The findings suggest that: (i) the stimulation of PGI2.biosynthesis is mediated byCa +, (ii) increased cAMP inhibits PGI2 production, and (iii) cAMP phosphodiesterase activity modulates PGI2-induced increases in the intracellular con- centration of cAMP. Prostacyclin (PGI2) is a potent vasodilator and inhibitor of plate-" @default.
- W2555772000 created "2016-11-30" @default.
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- W2555772000 date "2016-01-01" @default.
- W2555772000 modified "2023-09-26" @default.
- W2555772000 title "Role of Ca2+ and cyclic AMP in the of prostacyclin by the vascular end" @default.
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