Matches in SemOpenAlex for { <https://semopenalex.org/work/W2555780791> ?p ?o ?g. }
- W2555780791 endingPage "14940" @default.
- W2555780791 startingPage "14931" @default.
- W2555780791 abstract "We have developed a high resolution correlative method involving cryo-soft X-ray tomography (cryo-SXT) and stochastic optical reconstruction microscopy (STORM), which provides information in three dimensions on large cellular volumes at 70 nm resolution. Cryo-SXT morphologically identified and localized aggregations of carbon-rich materials. STORM identified specific markers on the desired epitopes, enabling colocalization between the identified objects, in this case cholesterol crystals, and the cellular environment. The samples were studied under ambient and cryogenic conditions without dehydration or heavy metal staining. The early events of cholesterol crystal development were investigated in relation to atherosclerosis, using as model macrophage cell cultures enriched with LDL particles. Atherosclerotic plaques build up in arteries in a slow process involving cholesterol crystal accumulation. Cholesterol crystal deposition is a crucial stage in the pathological cascade. Our results show that cholesterol crystals can be identified and imaged at a very early stage on the cell plasma membrane and in intracellular locations. This technique can in principle be applied to other biological samples where specific molecular identification is required in conjunction with high resolution 3D-imaging." @default.
- W2555780791 created "2016-11-30" @default.
- W2555780791 creator A5036815532 @default.
- W2555780791 creator A5048585838 @default.
- W2555780791 creator A5052255439 @default.
- W2555780791 creator A5056827342 @default.
- W2555780791 creator A5056897854 @default.
- W2555780791 creator A5058149110 @default.
- W2555780791 creator A5072554655 @default.
- W2555780791 creator A5072687480 @default.
- W2555780791 creator A5078784635 @default.
- W2555780791 date "2016-11-08" @default.
- W2555780791 modified "2023-10-17" @default.
- W2555780791 title "Development of Correlative Cryo-soft X-ray Tomography and Stochastic Reconstruction Microscopy. A Study of Cholesterol Crystal Early Formation in Cells" @default.
- W2555780791 cites W1487922209 @default.
- W2555780791 cites W1507921386 @default.
- W2555780791 cites W1508282658 @default.
- W2555780791 cites W1559879624 @default.
- W2555780791 cites W18060272 @default.
- W2555780791 cites W1840895580 @default.
- W2555780791 cites W1973587949 @default.
- W2555780791 cites W1979526109 @default.
- W2555780791 cites W1985294414 @default.
- W2555780791 cites W1988826338 @default.
- W2555780791 cites W1992144292 @default.
- W2555780791 cites W1995226155 @default.
- W2555780791 cites W1996586859 @default.
- W2555780791 cites W1997664070 @default.
- W2555780791 cites W1999569550 @default.
- W2555780791 cites W2005272468 @default.
- W2555780791 cites W2008747763 @default.
- W2555780791 cites W2012037497 @default.
- W2555780791 cites W2017404172 @default.
- W2555780791 cites W2025042015 @default.
- W2555780791 cites W2025587700 @default.
- W2555780791 cites W2028545985 @default.
- W2555780791 cites W2029709159 @default.
- W2555780791 cites W2034029712 @default.
- W2555780791 cites W2036181226 @default.
- W2555780791 cites W2036670100 @default.
- W2555780791 cites W2042809741 @default.
- W2555780791 cites W2048815642 @default.
- W2555780791 cites W2049720231 @default.
- W2555780791 cites W2064398178 @default.
- W2555780791 cites W2067397226 @default.
- W2555780791 cites W2068065241 @default.
- W2555780791 cites W2070699680 @default.
- W2555780791 cites W2072958209 @default.
- W2555780791 cites W2095180250 @default.
- W2555780791 cites W2096200556 @default.
- W2555780791 cites W2099994928 @default.
- W2555780791 cites W2101584400 @default.
- W2555780791 cites W2108041714 @default.
- W2555780791 cites W2113848462 @default.
- W2555780791 cites W2114598596 @default.
- W2555780791 cites W2118180586 @default.
- W2555780791 cites W2119244755 @default.
- W2555780791 cites W2119984885 @default.
- W2555780791 cites W2120626817 @default.
- W2555780791 cites W2121946692 @default.
- W2555780791 cites W2132090185 @default.
- W2555780791 cites W2155236033 @default.
- W2555780791 cites W2158671656 @default.
- W2555780791 cites W2168816122 @default.
- W2555780791 cites W2293502639 @default.
- W2555780791 cites W2312226411 @default.
- W2555780791 cites W2333058604 @default.
- W2555780791 cites W2340744737 @default.
- W2555780791 cites W2345779952 @default.
- W2555780791 cites W2470151853 @default.
- W2555780791 cites W40731526 @default.
- W2555780791 cites W819264700 @default.
- W2555780791 doi "https://doi.org/10.1021/jacs.6b07584" @default.
- W2555780791 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6530486" @default.
- W2555780791 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27934213" @default.
- W2555780791 hasPublicationYear "2016" @default.
- W2555780791 type Work @default.
- W2555780791 sameAs 2555780791 @default.
- W2555780791 citedByCount "32" @default.
- W2555780791 countsByYear W25557807912017 @default.
- W2555780791 countsByYear W25557807912018 @default.
- W2555780791 countsByYear W25557807912019 @default.
- W2555780791 countsByYear W25557807912020 @default.
- W2555780791 countsByYear W25557807912021 @default.
- W2555780791 countsByYear W25557807912022 @default.
- W2555780791 countsByYear W25557807912023 @default.
- W2555780791 crossrefType "journal-article" @default.
- W2555780791 hasAuthorship W2555780791A5036815532 @default.
- W2555780791 hasAuthorship W2555780791A5048585838 @default.
- W2555780791 hasAuthorship W2555780791A5052255439 @default.
- W2555780791 hasAuthorship W2555780791A5056827342 @default.
- W2555780791 hasAuthorship W2555780791A5056897854 @default.
- W2555780791 hasAuthorship W2555780791A5058149110 @default.
- W2555780791 hasAuthorship W2555780791A5072554655 @default.
- W2555780791 hasAuthorship W2555780791A5072687480 @default.
- W2555780791 hasAuthorship W2555780791A5078784635 @default.
- W2555780791 hasBestOaLocation W25557807912 @default.
- W2555780791 hasConcept C120665830 @default.