Matches in SemOpenAlex for { <https://semopenalex.org/work/W2555998423> ?p ?o ?g. }
- W2555998423 endingPage "139" @default.
- W2555998423 startingPage "132" @default.
- W2555998423 abstract "TGF-βs are multifunctional cytokines, but their roles in human renal homeostasis are not fully understood. This study investigated the role of TGF-β1 in the movement of human renal proximal tubular epithelial cells (PTECs) in a three-dimensional (3D) model. HKC-8 cells, a human PTEC line, were grown in a 3D collagen culture system. Cell movement was observed under a microscope. The gene expression was examined using PCR Arrays or qRT-PCR, and protein levels by Western blot. Here, we showed that the tight junction structure formed between adjacent cells of a HKC-8 cell colony in 3D cultures, and TGF-β1 stimulated their movement, evidenced by the appearance of fingerlike pseudopodia in the leader cells at the edge of the colonies. The cell movement of these human PTECs was correlated with up-regulation of both MMP2 and MMP9 and down-regulation or inactivation of PLAUR and PTK2B. Analysis of TGF-β signaling targets confirmed autocrine production of TGF-β2 and its cleaving enzyme furin as well as SNAI1 by TGF-β1stimulation. Knockdown of TGF-β2 expression disrupted TGF-β1-stimulated PTEC invasiveness, which was correlated with the down-regulation of MMP2 and MMP9. In conclusion, the activation of TGF-β receptor autocrine signaling by up-regulated TGF-β2 may play a pivotal role in TGF-β1-induced human PTEC movement, which could be mediated at least by both MMP2 and MMP9." @default.
- W2555998423 created "2016-11-30" @default.
- W2555998423 creator A5000576244 @default.
- W2555998423 creator A5001632271 @default.
- W2555998423 creator A5019673550 @default.
- W2555998423 creator A5072833715 @default.
- W2555998423 creator A5088326586 @default.
- W2555998423 date "2017-01-01" @default.
- W2555998423 modified "2023-09-23" @default.
- W2555998423 title "TGF-β1 stimulates movement of renal proximal tubular epithelial cells in a three-dimensional cell culture via an autocrine TGF-β2 production" @default.
- W2555998423 cites W110108558 @default.
- W2555998423 cites W1215049722 @default.
- W2555998423 cites W1503110580 @default.
- W2555998423 cites W1524114372 @default.
- W2555998423 cites W174053276 @default.
- W2555998423 cites W1900562057 @default.
- W2555998423 cites W1952271883 @default.
- W2555998423 cites W1967120404 @default.
- W2555998423 cites W1967774089 @default.
- W2555998423 cites W1971358122 @default.
- W2555998423 cites W1971799714 @default.
- W2555998423 cites W1977200818 @default.
- W2555998423 cites W1977303408 @default.
- W2555998423 cites W1979877257 @default.
- W2555998423 cites W1981941925 @default.
- W2555998423 cites W1993851092 @default.
- W2555998423 cites W2009729476 @default.
- W2555998423 cites W2019535584 @default.
- W2555998423 cites W2019866887 @default.
- W2555998423 cites W2024431958 @default.
- W2555998423 cites W2030524888 @default.
- W2555998423 cites W2038563972 @default.
- W2555998423 cites W2043156676 @default.
- W2555998423 cites W2048974061 @default.
- W2555998423 cites W2059600600 @default.
- W2555998423 cites W2062326040 @default.
- W2555998423 cites W2064527387 @default.
- W2555998423 cites W2086230321 @default.
- W2555998423 cites W2089207274 @default.
- W2555998423 cites W2092288827 @default.
- W2555998423 cites W2093585643 @default.
- W2555998423 cites W2106887481 @default.
- W2555998423 cites W2116759738 @default.
- W2555998423 cites W2119315749 @default.
- W2555998423 cites W2124653487 @default.
- W2555998423 cites W2125858978 @default.
- W2555998423 cites W2128563034 @default.
- W2555998423 cites W2129622487 @default.
- W2555998423 cites W2132971099 @default.
- W2555998423 cites W2133569303 @default.
- W2555998423 cites W2134080866 @default.
- W2555998423 cites W2136819827 @default.
- W2555998423 cites W2139511511 @default.
- W2555998423 cites W2140474512 @default.
- W2555998423 cites W2141700579 @default.
- W2555998423 cites W2143232800 @default.
- W2555998423 cites W2143880941 @default.
- W2555998423 cites W2145502337 @default.
- W2555998423 cites W2154251974 @default.
- W2555998423 cites W2154494910 @default.
- W2555998423 cites W2157790880 @default.
- W2555998423 cites W2161361346 @default.
- W2555998423 cites W2162804077 @default.
- W2555998423 cites W2189664213 @default.
- W2555998423 cites W2261311525 @default.
- W2555998423 cites W2322915906 @default.
- W2555998423 cites W2342679639 @default.
- W2555998423 cites W2343478579 @default.
- W2555998423 cites W2375726982 @default.
- W2555998423 cites W2466225158 @default.
- W2555998423 cites W4229646124 @default.
- W2555998423 doi "https://doi.org/10.1016/j.yexcr.2016.11.014" @default.
- W2555998423 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27880866" @default.
- W2555998423 hasPublicationYear "2017" @default.
- W2555998423 type Work @default.
- W2555998423 sameAs 2555998423 @default.
- W2555998423 citedByCount "3" @default.
- W2555998423 countsByYear W25559984232019 @default.
- W2555998423 crossrefType "journal-article" @default.
- W2555998423 hasAuthorship W2555998423A5000576244 @default.
- W2555998423 hasAuthorship W2555998423A5001632271 @default.
- W2555998423 hasAuthorship W2555998423A5019673550 @default.
- W2555998423 hasAuthorship W2555998423A5072833715 @default.
- W2555998423 hasAuthorship W2555998423A5088326586 @default.
- W2555998423 hasConcept C104317684 @default.
- W2555998423 hasConcept C118131993 @default.
- W2555998423 hasConcept C127561419 @default.
- W2555998423 hasConcept C128240485 @default.
- W2555998423 hasConcept C181199279 @default.
- W2555998423 hasConcept C2775997469 @default.
- W2555998423 hasConcept C2777265534 @default.
- W2555998423 hasConcept C54355233 @default.
- W2555998423 hasConcept C55493867 @default.
- W2555998423 hasConcept C62478195 @default.
- W2555998423 hasConcept C81885089 @default.
- W2555998423 hasConcept C86803240 @default.
- W2555998423 hasConcept C95444343 @default.
- W2555998423 hasConceptScore W2555998423C104317684 @default.