Matches in SemOpenAlex for { <https://semopenalex.org/work/W2556287350> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2556287350 abstract "We report the use of direct-conductivity sensing as a means for measuring mobile- phase composition and flow rate inside an HPLC chromatography chip. Instantaneous on-chip solvent percentage during gradient elution is determined from the measured mobile-phase conductivity. The method can show changes in mobile phase organic solvent percentage of I%, even at 90% acetonitrile. In addition, flow rates in the range of 100 to 1000 nL/min are determined by measuring the time-of-flight of a heat pulse as it moves from a heater trace to embedded conductivity sensing electrode. Microfluidic devices employing partition chromatography have been shown to be capable of efficient separations of biomolecules such as peptides and proteins ( 11. Most importantly, the narrow bandwidths produced by short, small diameter separation chamrels in microfluidic chips allow analysis of exceptionally low sample volnmes with concentration sensitivities similar to those of traditional, larger columns. Chromatographic separation requires a mobile-phase pump. Pumps currently in use were originally designed for standard or narrow bore columns and employ splitters to produce the sub-ylimin flow rates required for microfluidic nanoflow devices. Although these pumps work well for this application, the time required for solvent changes at the pump proportioning valve to reach the microfluidic chromatography channel is larger in comparison to standard bore ~01~s. In addition, improper tubing and fitting connections between the pump and microfluidic device can cause leaks that may go undetected due to the extremely low flow rates used. For these reasons, on-chip sensors that can measure gradient solvent strength as well as actual on-chip flow rate are used to monitor mobile phase conditions in the device, rather than only at the pump." @default.
- W2556287350 created "2016-11-30" @default.
- W2556287350 creator A5053727592 @default.
- W2556287350 creator A5067279326 @default.
- W2556287350 creator A5083609319 @default.
- W2556287350 date "2003-01-01" @default.
- W2556287350 modified "2023-09-27" @default.
- W2556287350 title "USE OF CONDUCTIVITY ELECTRODES AS ON-CHIP SENSORS IN CHROMATOGRAPHIC MICROFLUIDIC DEVICES" @default.
- W2556287350 cites W2031991917 @default.
- W2556287350 hasPublicationYear "2003" @default.
- W2556287350 type Work @default.
- W2556287350 sameAs 2556287350 @default.
- W2556287350 citedByCount "0" @default.
- W2556287350 crossrefType "journal-article" @default.
- W2556287350 hasAuthorship W2556287350A5053727592 @default.
- W2556287350 hasAuthorship W2556287350A5067279326 @default.
- W2556287350 hasAuthorship W2556287350A5083609319 @default.
- W2556287350 hasConcept C113196181 @default.
- W2556287350 hasConcept C119599485 @default.
- W2556287350 hasConcept C121332964 @default.
- W2556287350 hasConcept C127413603 @default.
- W2556287350 hasConcept C131540310 @default.
- W2556287350 hasConcept C138942068 @default.
- W2556287350 hasConcept C147789679 @default.
- W2556287350 hasConcept C165005293 @default.
- W2556287350 hasConcept C171250308 @default.
- W2556287350 hasConcept C172120300 @default.
- W2556287350 hasConcept C185592680 @default.
- W2556287350 hasConcept C192562407 @default.
- W2556287350 hasConcept C2777463227 @default.
- W2556287350 hasConcept C43617362 @default.
- W2556287350 hasConcept C62520636 @default.
- W2556287350 hasConcept C73826308 @default.
- W2556287350 hasConcept C8673954 @default.
- W2556287350 hasConceptScore W2556287350C113196181 @default.
- W2556287350 hasConceptScore W2556287350C119599485 @default.
- W2556287350 hasConceptScore W2556287350C121332964 @default.
- W2556287350 hasConceptScore W2556287350C127413603 @default.
- W2556287350 hasConceptScore W2556287350C131540310 @default.
- W2556287350 hasConceptScore W2556287350C138942068 @default.
- W2556287350 hasConceptScore W2556287350C147789679 @default.
- W2556287350 hasConceptScore W2556287350C165005293 @default.
- W2556287350 hasConceptScore W2556287350C171250308 @default.
- W2556287350 hasConceptScore W2556287350C172120300 @default.
- W2556287350 hasConceptScore W2556287350C185592680 @default.
- W2556287350 hasConceptScore W2556287350C192562407 @default.
- W2556287350 hasConceptScore W2556287350C2777463227 @default.
- W2556287350 hasConceptScore W2556287350C43617362 @default.
- W2556287350 hasConceptScore W2556287350C62520636 @default.
- W2556287350 hasConceptScore W2556287350C73826308 @default.
- W2556287350 hasConceptScore W2556287350C8673954 @default.
- W2556287350 hasLocation W25562873501 @default.
- W2556287350 hasOpenAccess W2556287350 @default.
- W2556287350 hasPrimaryLocation W25562873501 @default.
- W2556287350 hasRelatedWork W1992323017 @default.
- W2556287350 hasRelatedWork W2010268228 @default.
- W2556287350 hasRelatedWork W2030349910 @default.
- W2556287350 hasRelatedWork W2076656699 @default.
- W2556287350 hasRelatedWork W2171775431 @default.
- W2556287350 hasRelatedWork W2183717028 @default.
- W2556287350 hasRelatedWork W2537862911 @default.
- W2556287350 hasRelatedWork W3169304765 @default.
- W2556287350 hasRelatedWork W3196438618 @default.
- W2556287350 hasRelatedWork W3211743640 @default.
- W2556287350 hasRelatedWork W93270370 @default.
- W2556287350 hasRelatedWork W1543447247 @default.
- W2556287350 hasRelatedWork W1809120971 @default.
- W2556287350 hasRelatedWork W1814207448 @default.
- W2556287350 hasRelatedWork W2309154596 @default.
- W2556287350 hasRelatedWork W2785077442 @default.
- W2556287350 hasRelatedWork W2839304070 @default.
- W2556287350 hasRelatedWork W2861572767 @default.
- W2556287350 hasRelatedWork W2988190675 @default.
- W2556287350 hasRelatedWork W949683727 @default.
- W2556287350 isParatext "false" @default.
- W2556287350 isRetracted "false" @default.
- W2556287350 magId "2556287350" @default.
- W2556287350 workType "article" @default.