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• W2556356859 abstract "Figure S1. Training of extracellular model parameters. 50 μM DPTA NONOate was delivered to cell-free media (MOPS minimal media with 10 mM glucose) at 50 and 0 μM O2, and [NO·] was measured (solid red lines; mean of 3 independent experiments, with light red shading representing the SEM). For the 0 μM O2 condition, the media pH was adjusted to 7.2 (from 7.4) with HCl, to mimic the slightly acidified conditions measured in the bioreactor culture for anaerobic assays with EHEC. Model parameters specific to the experimental apparatus and media conditions (kNO·-O2, kLaNO·, and kNONOate) were optimized on the [NO·] curve measured at 50 μM O2 (dashed black line using the best-fit parameter set, with gray shading representing the range of viable parameter sets with ER < 10). Since a decrease in pH increases the rate of NONOate dissociation, the kNONOate parameter was released and trained on [NO·] measured in the pH-adjusted media at 0 μM O2. The simulation result using the trained kNONOate parameter is shown (dashed black line, obtained using the best-fit parameter value, with gray shading representing viable parameter values with ER < 10). Figure S2. Training of parameters associated with the respiratory module. TUV hmp + /norV + in midexponential phase were delivered to the bioreactor to an OD600 of 0.05, and the O2 consumption was measured (solid purple line; mean of 3 independent experiments with light purple shading representing the SEM). Model parameters associated with the aerobic respiratory module (NADH dehydrogenases and cytochrome ubiquinol oxidases) were trained on the measured [O2]. The simulated [O2] generated by the trained model is shown (dashed black line was obtained using the best-fit parameter set, with gray shading representing the range of viable parameter sets with ER < 10)." @default.
• W2556356859 created "2016-11-30" @default.
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• W2556356859 date "2016-01-01" @default.
• W2556356859 modified "2023-09-26" @default.
• W2556356859 title "Supplementary Materials: Construction and Experimental Validation of a Quantitative Kinetic Model of Nitric Oxide Stress in Enterohemorrhagic Escherichia coli O157:H7" @default.
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