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• W2556446510 abstract "SUMMARY The growing interest in the substitution of traditional food preservatives, both antimicrobials and antioxidants, by natural ones has fostered the screening of plant materials. In this context, essential oils (EOs) and their components are known to exhibit wide range of antimicrobial activities namely against pathogens. Nevertheless, there is no data concerning the examination of their effects on beneficial normal digestive microflora or on the starter cultures largely used in many industries. This work describes, for the first time, the use of a multiparametric flow cytometry technique to assess the mode of action, at the single cell level, of Melaleuca armillaris and Lavandula dentata EOs against two lactic acid bacteria (LAB) strains. Using an automated microtiter assay (Bioscreen C), bacteriostatic or bactericidal effects were noticed depending on the studied strain and on the applied concentration of each of the EOs. The mathematical modelling of the kinetics showed that in presence of increasing concentrations of a given EO, the lag phases of growth were extended (1.55% to 94.3%) and both the growth rate and final cell density were reduced. Variations depending on the strain were noticed. Live/dead assays of the multiparametric flow cytometry technique, combining Carboxyfluorescein diacetate (cFDA) and Propidium Iodide (PI) fluorescent probes, were done by dual staining of each sample culture to differentiate viable, dead and stressed cells. The behaviour of each strain, in presence of increasing concentrations of each of the EOs, was evaluated by quantifying the relative percentages of each subpopulation during the cultivation period (3 days). Results displayed disparate patterns of" @default.
• W2556446510 created "2016-11-30" @default.
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• W2556446510 date "2009-01-01" @default.
• W2556446510 modified "2023-09-27" @default.
• W2556446510 title "STUDY ON THE EFFECTS OF LAVENDULA DENTATA AND MELALEUCA ARMILLARIS ESSENTIAL OILS ON TWO LAB STRAINS BY A MULTIPARAMETRIC FLOW CYTOMETRY TECHNIQUE VIŠEPARAMETARSKA PROTOČNA CITOMETRIJA KAO MOĆNO ORUĐE ZA PROCENU MEHANIZMA UTICAJA ESENCIJALNIH ULJA NA LABORATORIJSKI UREĐAJ ZA CEĐENJE" @default.
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• W2556446510 hasPublicationYear "2009" @default.
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