FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2556614114> ?p ?o ?g. }

• W2556614114 abstract "ABSTRACT More than 75 “species-level” phylotypes of spirochete bacteria belonging to the genus Treponema reside within the human oral cavity. The majority of these oral treponeme phylotypes correspond to as-yet-uncultivated taxa or strains of uncertain standing in taxonomy. Here, we analyze phylogenetic and taxonomic relationships between oral treponeme strains using a multilocus sequence analysis (MLSA) scheme based on the highly conserved 16S rRNA, pyrH , recA , and flaA genes. We utilized this MLSA scheme to analyze genetic data from a curated collection of oral treponeme strains ( n = 71) of diverse geographical origins. This comprises phylogroup 1 ( n = 23) and phylogroup 2 ( n = 48) treponeme strains, including all relevant American Type Culture Collection reference strains. The taxonomy of all strains was confirmed or inferred via the analysis of ca. 1,450-bp 16S rRNA gene sequences using a combination of bioinformatic and phylogenetic approaches. Taxonomic and phylogenetic relationships between the respective treponeme strains were further investigated by analyzing individual and concatenated flaA (1,074-nucleotide [nt]), recA (1,377-nt), and pyrH (696-nt) gene sequence data sets. Our data confirmed the species differentiation between Treponema denticola ( n = 41) and Treponema putidum ( n = 7) strains. Notably, our results clearly supported the differentiation of the 23 phylogroup 1 treponeme strains into five distinct “species-level” phylotypes. These respectively corresponded to “ Treponema vincentii ” ( n = 11), Treponema medium ( n = 1), “ Treponema sinensis ” ( Treponema sp. IA; n = 4), Treponema sp. IB ( n = 3), and Treponema sp. IC ( n = 4). In conclusion, our MLSA-based approach can be used to effectively discriminate oral treponeme taxa, confirm taxonomic assignment, and enable the delineation of species boundaries with high confidence. IMPORTANCE Periodontal diseases are caused by persistent polymicrobial biofilm infections of the gums and underlying tooth-supporting structures and have a complex and variable etiology. Although Treponema denticola is strongly associated with periodontal diseases, the etiological roles of other treponeme species/phylotypes are less well defined. This is due to a paucity of formal species descriptions and a poor understanding of genetic relationships between oral treponeme taxa. Our study directly addresses these issues. It represents one of the most comprehensive analyses of oral treponeme strains performed to date, including isolates from North America, Europe, and Asia. We envisage that our results will greatly facilitate future metagenomic efforts aimed at characterizing the clinical distributions of oral treponeme species/phylotypes, helping investigators to establish a more detailed understanding of their etiological roles in periodontal diseases and other infectious diseases. Our results are also directly relevant to various polymicrobial tissue infections in animals, which also involve treponeme populations." @default.
• W2556614114 created "2016-11-30" @default.
• W2556614114 creator A5009922018 @default.
• W2556614114 creator A5017803324 @default.
• W2556614114 creator A5022424644 @default.
• W2556614114 creator A5061150866 @default.
• W2556614114 creator A5063168870 @default.
• W2556614114 creator A5063852817 @default.
• W2556614114 creator A5076379245 @default.
• W2556614114 date "2017-02-01" @default.
• W2556614114 modified "2023-10-03" @default.
• W2556614114 title "Multilocus Sequence Analysis of Phylogroup 1 and 2 Oral Treponeme Strains" @default.
• W2556614114 cites W1556725413 @default.
• W2556614114 cites W1848056131 @default.
• W2556614114 cites W1905529155 @default.
• W2556614114 cites W1925896619 @default.
• W2556614114 cites W1943807775 @default.
• W2556614114 cites W1964525389 @default.
• W2556614114 cites W1964968238 @default.
• W2556614114 cites W1965660108 @default.
• W2556614114 cites W1969122489 @default.
• W2556614114 cites W1978260581 @default.
• W2556614114 cites W1985663971 @default.
• W2556614114 cites W1993016060 @default.
• W2556614114 cites W1999591895 @default.
• W2556614114 cites W2003369514 @default.
• W2556614114 cites W2005931515 @default.
• W2556614114 cites W2006278170 @default.
• W2556614114 cites W2015888504 @default.
• W2556614114 cites W2016815678 @default.
• W2556614114 cites W2016861192 @default.
• W2556614114 cites W2017133098 @default.
• W2556614114 cites W2019094601 @default.
• W2556614114 cites W2019115123 @default.
• W2556614114 cites W2029026026 @default.
• W2556614114 cites W2032058289 @default.
• W2556614114 cites W2032857451 @default.
• W2556614114 cites W2034832242 @default.
• W2556614114 cites W2045732535 @default.
• W2556614114 cites W2053277647 @default.
• W2556614114 cites W2053855484 @default.
• W2556614114 cites W2054779467 @default.
• W2556614114 cites W2055592929 @default.
• W2556614114 cites W2059492480 @default.
• W2556614114 cites W2062780004 @default.
• W2556614114 cites W2064079765 @default.
• W2556614114 cites W2066915528 @default.
• W2556614114 cites W2075123070 @default.
• W2556614114 cites W2085991749 @default.
• W2556614114 cites W2092338165 @default.
• W2556614114 cites W2093250796 @default.
• W2556614114 cites W2096571732 @default.
• W2556614114 cites W2099107110 @default.
• W2556614114 cites W2103395792 @default.
• W2556614114 cites W2106552585 @default.
• W2556614114 cites W2107014059 @default.
• W2556614114 cites W2107621690 @default.
• W2556614114 cites W2110899053 @default.
• W2556614114 cites W2112022892 @default.
• W2556614114 cites W2114936248 @default.
• W2556614114 cites W2115699469 @default.
• W2556614114 cites W2120611093 @default.
• W2556614114 cites W2121573211 @default.
• W2556614114 cites W2121748291 @default.
• W2556614114 cites W2127308212 @default.
• W2556614114 cites W2128113743 @default.
• W2556614114 cites W2128573889 @default.
• W2556614114 cites W2131807998 @default.
• W2556614114 cites W2132926880 @default.
• W2556614114 cites W2134292086 @default.
• W2556614114 cites W2139246422 @default.
• W2556614114 cites W2139736670 @default.
• W2556614114 cites W2144220515 @default.
• W2556614114 cites W2144555116 @default.
• W2556614114 cites W2146237557 @default.
• W2556614114 cites W2147221919 @default.
• W2556614114 cites W2147687946 @default.
• W2556614114 cites W2147710356 @default.
• W2556614114 cites W2149160071 @default.
• W2556614114 cites W2150121420 @default.
• W2556614114 cites W2154845780 @default.
• W2556614114 cites W2155065346 @default.
• W2556614114 cites W2157107389 @default.
• W2556614114 cites W2157770738 @default.
• W2556614114 cites W2160559534 @default.
• W2556614114 cites W2161444534 @default.
• W2556614114 cites W2162057114 @default.
• W2556614114 cites W2164989301 @default.
• W2556614114 cites W2168719962 @default.
• W2556614114 cites W2169325493 @default.
• W2556614114 cites W2171571559 @default.
• W2556614114 cites W2202326042 @default.
• W2556614114 cites W2491297437 @default.
• W2556614114 cites W4230126804 @default.
• W2556614114 cites W4240755130 @default.
• W2556614114 cites W4243968414 @default.
• W2556614114 cites W91521331 @default.
• W2556614114 doi "https://doi.org/10.1128/aem.02499-16" @default.
• W2556614114 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5244305" @default.
• W2556614114 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27864174" @default.

• next