FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2556843357> ?p ?o ?g. }

• W2556843357 endingPage "67" @default.
• W2556843357 startingPage "60" @default.
• W2556843357 abstract "The diheme enzyme MauG utilizes H2O2 to perform oxidative posttranslational modification on a protein substrate. A bis-Fe(IV) species of MauG was previously identified as a key intermediate in this reaction. Heterolytic cleavage of the OO bond of H2O2 drives the formation of the bis-Fe(IV) intermediate. In this work, we tested a hypothesis that a glutamate residue, Glu113 in the distal pocket of the pentacoordinate heme of MauG, facilitates heterolytic OO bond cleavage, thereby leading to bis-Fe(IV) formation. This hypothesis was proposed based on sequence alignment and structural comparison with other H2O2-utilizing hemoenzymes, especially those from the diheme enzyme superfamily that MauG belongs to. Electron paramagnetic resonance (EPR) characterization of the reaction between MauG and H2O2 revealed that mutation of Glu113 inhibited heterolytic OO bond cleavage, in agreement with our hypothesis. This result was further confirmed by the HPLC study in which an analog of H2O2, cumene hydroperoxide, was used to probe the pattern of OO bond cleavage. Together, our data suggest that Glu113 functions as an acid-base catalyst to assist heterolytic OO bond cleavage during the early stage of the catalytic reaction. This work advances our mechanistic understanding of the H2O2-activation process during bis-Fe(IV) formation in MauG." @default.
• W2556843357 created "2016-11-30" @default.
• W2556843357 creator A5004584299 @default.
• W2556843357 creator A5069795151 @default.
• W2556843357 creator A5079431165 @default.
• W2556843357 date "2017-02-01" @default.
• W2556843357 modified "2023-09-24" @default.
• W2556843357 title "Heterolytic OO bond cleavage: Functional role of Glu113 during bis-Fe(IV) formation in MauG" @default.
• W2556843357 cites W114440940 @default.
• W2556843357 cites W1511153971 @default.
• W2556843357 cites W1519790720 @default.
• W2556843357 cites W1535104931 @default.
• W2556843357 cites W1566303386 @default.
• W2556843357 cites W1589783447 @default.
• W2556843357 cites W1597212776 @default.
• W2556843357 cites W1866116049 @default.
• W2556843357 cites W1969290812 @default.
• W2556843357 cites W1972433518 @default.
• W2556843357 cites W1972476095 @default.
• W2556843357 cites W1975262546 @default.
• W2556843357 cites W1976694026 @default.
• W2556843357 cites W1982081525 @default.
• W2556843357 cites W1993172959 @default.
• W2556843357 cites W2000508647 @default.
• W2556843357 cites W2009081664 @default.
• W2556843357 cites W2010967782 @default.
• W2556843357 cites W2011374678 @default.
• W2556843357 cites W2023023571 @default.
• W2556843357 cites W2026036565 @default.
• W2556843357 cites W2028026107 @default.
• W2556843357 cites W2030661553 @default.
• W2556843357 cites W2030817463 @default.
• W2556843357 cites W2039425214 @default.
• W2556843357 cites W2040111466 @default.
• W2556843357 cites W2057453457 @default.
• W2556843357 cites W2063337814 @default.
• W2556843357 cites W2067472218 @default.
• W2556843357 cites W2072676813 @default.
• W2556843357 cites W2079244126 @default.
• W2556843357 cites W2079310705 @default.
• W2556843357 cites W2079739873 @default.
• W2556843357 cites W2081526839 @default.
• W2556843357 cites W2086219034 @default.
• W2556843357 cites W2086847055 @default.
• W2556843357 cites W2090933843 @default.
• W2556843357 cites W2118132519 @default.
• W2556843357 cites W2124124969 @default.
• W2556843357 cites W2140041862 @default.
• W2556843357 cites W2149364216 @default.
• W2556843357 cites W2150934217 @default.
• W2556843357 cites W2156448192 @default.
• W2556843357 cites W2160208439 @default.
• W2556843357 cites W2165898505 @default.
• W2556843357 cites W2317873538 @default.
• W2556843357 cites W2330676190 @default.
• W2556843357 doi "https://doi.org/10.1016/j.jinorgbio.2016.11.013" @default.
• W2556843357 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5191938" @default.
• W2556843357 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27907864" @default.
• W2556843357 hasPublicationYear "2017" @default.
• W2556843357 type Work @default.
• W2556843357 sameAs 2556843357 @default.
• W2556843357 citedByCount "3" @default.
• W2556843357 countsByYear W25568433572018 @default.
• W2556843357 countsByYear W25568433572023 @default.
• W2556843357 crossrefType "journal-article" @default.
• W2556843357 hasAuthorship W2556843357A5004584299 @default.
• W2556843357 hasAuthorship W2556843357A5069795151 @default.
• W2556843357 hasAuthorship W2556843357A5079431165 @default.
• W2556843357 hasBestOaLocation W25568433572 @default.
• W2556843357 hasConcept C121332964 @default.
• W2556843357 hasConcept C127413603 @default.
• W2556843357 hasConcept C161790260 @default.
• W2556843357 hasConcept C175156509 @default.
• W2556843357 hasConcept C185592680 @default.
• W2556843357 hasConcept C187320778 @default.
• W2556843357 hasConcept C187961010 @default.
• W2556843357 hasConcept C3044715 @default.
• W2556843357 hasConcept C43369102 @default.
• W2556843357 hasConcept C44578547 @default.
• W2556843357 hasConcept C46141821 @default.
• W2556843357 hasConcept C55493867 @default.
• W2556843357 hasConcept C71240020 @default.
• W2556843357 hasConcept C75473681 @default.
• W2556843357 hasConceptScore W2556843357C121332964 @default.
• W2556843357 hasConceptScore W2556843357C127413603 @default.
• W2556843357 hasConceptScore W2556843357C161790260 @default.
• W2556843357 hasConceptScore W2556843357C175156509 @default.
• W2556843357 hasConceptScore W2556843357C185592680 @default.
• W2556843357 hasConceptScore W2556843357C187320778 @default.
• W2556843357 hasConceptScore W2556843357C187961010 @default.
• W2556843357 hasConceptScore W2556843357C3044715 @default.
• W2556843357 hasConceptScore W2556843357C43369102 @default.
• W2556843357 hasConceptScore W2556843357C44578547 @default.
• W2556843357 hasConceptScore W2556843357C46141821 @default.
• W2556843357 hasConceptScore W2556843357C55493867 @default.
• W2556843357 hasConceptScore W2556843357C71240020 @default.
• W2556843357 hasConceptScore W2556843357C75473681 @default.
• W2556843357 hasFunder F4320332161 @default.

• next