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- W2557069089 abstract "Due to the steadily increasing number of presumed herbicide resistant weed populations, the demand for rapid in-season tests is rising. In this study we present a new quantitative herbicide resistance test system based on chlorophyll fluorescence imaging analysis of photosynthesis related parameters. Herbicide resistant and susceptible populations of Alopecurus myosuroides (blackgrass) were cultivated in multiwell tissue culture plates containing nutrient agar and different dosages of fenoxaprop-P-ethyl and mesosulfuron+iodosulfuron. The maximum quantum efficiency of the PSI was measured 3 hours after transplanting (HAT) and then for seven days every 24 hours. Data of maximum quantum efficiency of the PSII were compared with conventional whole-plant pot tests and molecular tests for target-site mutations. It was possible to fit dose-response curves and calculate corresponding resistance factors for ED90 for all populations tested using the chlorophyll fluorescence imaging. It was possible to distinguish between resistant and susceptible populations. The results of the chlorophyll fluorescence imaging Chapter II Publications 11 corresponded well to the conventional whole-plant pot tests in the greenhouse. However, populations with verified target-site mutations did not differ from other herbicide resistant populations in the maximum quantum efficiency values of the PSII. We conclude that the chlorophyll fluorescence imaging provides reliable data on herbicide resistance for both modes of action tested in shorter time and using less space compared with conventional whole-plant pot tests in the greenhouse." @default.
- W2557069089 created "2016-11-30" @default.
- W2557069089 creator A5081797414 @default.
- W2557069089 date "2015-01-01" @default.
- W2557069089 modified "2023-09-27" @default.
- W2557069089 title "Integrated management, analysis of mechanisms and early detection of resistant populations of Alopecurus myosuroides HUDS. and Apera spica-venti L. Beauv." @default.
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