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• W2557182603 abstract "Background Many biotechnological and industrial applications can benefit from cold-adapted EglCs through increased efficiency of catalytic processes at low temperature. In our previous study, Citrobacter farmeri A1 which was isolated from a wood-inhabiting termite Reticulitermes labralis could secrete a cold-adapted EglC. However, its EglC was difficult to purify for enzymatic properties detection because of its low activity (0.8 U/ml). The objective of the present study was to clone and express the C. farmeri EglC gene in Escherichia coli to improve production level and determine the enzymatic properties of the recombinant enzyme. Methods The EglC gene was cloned from C. farmeri A1 by thermal asymmetric interlaced PCR. EglC was transformed into vector pET22b and functionally expressed in E. coli . The recombination protein EglC22b was purified for properties detection. Results SDS-PAGE revealed that the molecular mass of the recombinant endoglucanase was approximately 42 kDa. The activity of the E. coli pET22b-EglC crude extract was 9.5 U/ml. Additionally, it was active at pH 6.5–8.0 with an optimum pH of 7.0. The recombinant enzyme had an optimal temperature of 30–40 °C and exhibited >50% relative activity even at 5 °C, whereas it lost approximately 90% of its activity after incubation at 60 °C for 30 min. Its activity was enhanced by Co 2+ and Fe 3+ , but inhibited by Cd 2+ , Zn 2+ , Li + , Triton X-100, DMSO, acetonitrile, Tween 80, SDS, and EDTA. Conclusion These biochemical properties indicate that the recombinant enzyme is a cold-adapted endoglucanase that can be used for various industrial applications." @default.
• W2557182603 created "2016-11-30" @default.
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• W2557182603 date "2016-11-08" @default.
• W2557182603 modified "2023-10-16" @default.
• W2557182603 title "Cloning, expression and characterization of a cold-adapted endo-1, 4-<i>β</i>-glucanase from <i>Citrobacter farmeri</i> A1, a symbiotic bacterium of <i>Reticulitermes labralis</i>" @default.
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• W2557182603 doi "https://doi.org/10.7717/peerj.2679" @default.
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