Matches in SemOpenAlex for { <https://semopenalex.org/work/W2557283570> ?p ?o ?g. }
- W2557283570 endingPage "824" @default.
- W2557283570 startingPage "812" @default.
- W2557283570 abstract "Phosphate overaccumulator2 (PHO2) encodes a ubiquitin-conjugating E2 enzyme that is a major negative regulator of the inorganic phosphate (Pi)-starvation response-signaling pathway. A yeast two-hybrid (Y2H) screen in rice (Oryza sativa; Os) using OsPHO2 as bait revealed an interaction between OsPHO2 and two h-type thioredoxins, OsTrxh1 and OsTrxh4. These interactions were confirmed in vivo using bimolecular fluorescence complementation (BiFC) of OsPHO2 and OsTrxh1/h4 in rice protoplasts and by in vitro pull-down assays with 6His-tagged OsTrxh1/h4 and GST-tagged OsPHO2. Y2H assays revealed that amino acid Cys-445 of OsPHO2 and an N-terminal Cys in the WCGPC motif of Trxhs were required for the interaction. Split-ubiquitin Y2H analyses and BiFC assays in rice protoplasts confirmed the interaction of OsPHO2 with PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (OsPHF1), and PHOSPHATE1;2 (OsPHO1;2) in the endoplasmic reticulum and Golgi membrane system, where OsPHO2 mediates the degradation of OsPHF1 in both tobacco (Nicotiana benthamiana) leaves and rice seedlings. Characterization of rice pho2 complemented lines, transformed with an endogenous genomic OsPHO2 or OsPHO2C445S (a constitutively reduced form) fragment, indicated that OsPHO2C445S restored Pi concentration in rice to statistically significant lower levels compared to native OsPHO2 Moreover, the suppression of OsTrxh1 (knockdown and knockout) resulted in slightly higher Pi concentration than that of wild-type Nipponbare in leaves. These results demonstrate that OsPHO2 is under redox control by thioredoxins, which fine-tune its activity and link Pi homeostasis with redox balance in rice." @default.
- W2557283570 created "2016-12-08" @default.
- W2557283570 creator A5003832990 @default.
- W2557283570 creator A5013305975 @default.
- W2557283570 creator A5021660477 @default.
- W2557283570 creator A5030040052 @default.
- W2557283570 creator A5032285717 @default.
- W2557283570 creator A5034948499 @default.
- W2557283570 creator A5065230407 @default.
- W2557283570 creator A5069057903 @default.
- W2557283570 creator A5072240312 @default.
- W2557283570 creator A5086767799 @default.
- W2557283570 date "2016-11-28" @default.
- W2557283570 modified "2023-10-17" @default.
- W2557283570 title "Two h-Type Thioredoxins Interact with the E2 Ubiquitin Conjugase PHO2 to Fine-Tune Phosphate Homeostasis in Rice" @default.
- W2557283570 cites W1555938743 @default.
- W2557283570 cites W1569627963 @default.
- W2557283570 cites W1583232467 @default.
- W2557283570 cites W1584729364 @default.
- W2557283570 cites W1965979287 @default.
- W2557283570 cites W1969118015 @default.
- W2557283570 cites W1988691682 @default.
- W2557283570 cites W1989035193 @default.
- W2557283570 cites W2004833582 @default.
- W2557283570 cites W2012293947 @default.
- W2557283570 cites W2036330617 @default.
- W2557283570 cites W2050733201 @default.
- W2557283570 cites W2056461155 @default.
- W2557283570 cites W2060687391 @default.
- W2557283570 cites W2061081431 @default.
- W2557283570 cites W2062507088 @default.
- W2557283570 cites W2070207713 @default.
- W2557283570 cites W2079421360 @default.
- W2557283570 cites W2082051810 @default.
- W2557283570 cites W2085377827 @default.
- W2557283570 cites W2085814119 @default.
- W2557283570 cites W2104425028 @default.
- W2557283570 cites W2111145333 @default.
- W2557283570 cites W2114957961 @default.
- W2557283570 cites W2116122104 @default.
- W2557283570 cites W2117021101 @default.
- W2557283570 cites W2118335889 @default.
- W2557283570 cites W2118752893 @default.
- W2557283570 cites W2120396983 @default.
- W2557283570 cites W2121752230 @default.
- W2557283570 cites W2123633232 @default.
- W2557283570 cites W2127253105 @default.
- W2557283570 cites W2128034264 @default.
- W2557283570 cites W2135487705 @default.
- W2557283570 cites W2135513524 @default.
- W2557283570 cites W2136758019 @default.
- W2557283570 cites W2145753856 @default.
- W2557283570 cites W2146325157 @default.
- W2557283570 cites W2150518597 @default.
- W2557283570 cites W2150648093 @default.
- W2557283570 cites W2157743364 @default.
- W2557283570 cites W2159283576 @default.
- W2557283570 cites W2170246161 @default.
- W2557283570 cites W3024054742 @default.
- W2557283570 cites W4251086924 @default.
- W2557283570 doi "https://doi.org/10.1104/pp.16.01639" @default.
- W2557283570 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5210762" @default.
- W2557283570 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27895204" @default.
- W2557283570 hasPublicationYear "2016" @default.
- W2557283570 type Work @default.
- W2557283570 sameAs 2557283570 @default.
- W2557283570 citedByCount "37" @default.
- W2557283570 countsByYear W25572835702017 @default.
- W2557283570 countsByYear W25572835702018 @default.
- W2557283570 countsByYear W25572835702019 @default.
- W2557283570 countsByYear W25572835702020 @default.
- W2557283570 countsByYear W25572835702021 @default.
- W2557283570 countsByYear W25572835702022 @default.
- W2557283570 countsByYear W25572835702023 @default.
- W2557283570 crossrefType "journal-article" @default.
- W2557283570 hasAuthorship W2557283570A5003832990 @default.
- W2557283570 hasAuthorship W2557283570A5013305975 @default.
- W2557283570 hasAuthorship W2557283570A5021660477 @default.
- W2557283570 hasAuthorship W2557283570A5030040052 @default.
- W2557283570 hasAuthorship W2557283570A5032285717 @default.
- W2557283570 hasAuthorship W2557283570A5034948499 @default.
- W2557283570 hasAuthorship W2557283570A5065230407 @default.
- W2557283570 hasAuthorship W2557283570A5069057903 @default.
- W2557283570 hasAuthorship W2557283570A5072240312 @default.
- W2557283570 hasAuthorship W2557283570A5086767799 @default.
- W2557283570 hasBestOaLocation W25572835701 @default.
- W2557283570 hasConcept C104317684 @default.
- W2557283570 hasConcept C119062480 @default.
- W2557283570 hasConcept C123243835 @default.
- W2557283570 hasConcept C143065580 @default.
- W2557283570 hasConcept C158617107 @default.
- W2557283570 hasConcept C207583985 @default.
- W2557283570 hasConcept C2778543611 @default.
- W2557283570 hasConcept C2779222958 @default.
- W2557283570 hasConcept C55493867 @default.
- W2557283570 hasConcept C86803240 @default.
- W2557283570 hasConcept C95444343 @default.
- W2557283570 hasConceptScore W2557283570C104317684 @default.