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- W2557389600 abstract "Here we outline how to identify hydrogenase enzymes from metagenomic libraries through an activity-based screening approach. A metagenomic fosmid library is constructed in E. coli and the fosmids are transferred into a hydrogenase deletion mutant of Shewanella oneidensis (ΔhyaB) via triparental mating. If a fosmid exhibits hydrogen uptake activity, S. oneidensis' phenotype is restored and hydrogenase activity is indicated by a color change of the medium from yellow to colorless. This new method enables screening of 48 metagenomic fosmid clones in parallel." @default.
- W2557389600 created "2016-12-08" @default.
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- W2557389600 date "2016-11-30" @default.
- W2557389600 modified "2023-10-16" @default.
- W2557389600 title "Activity-Based Screening of Metagenomic Libraries for Hydrogenase Enzymes" @default.
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- W2557389600 doi "https://doi.org/10.1007/978-1-4939-6691-2_17" @default.
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