Matches in SemOpenAlex for { <https://semopenalex.org/work/W2557551977> ?p ?o ?g. }
- W2557551977 endingPage "190" @default.
- W2557551977 startingPage "190" @default.
- W2557551977 abstract "Benzoylformate decarboxylase (BFDC) and pyruvate decarboxylase (PDC) are thiamin diphosphate-dependent enzymes that share some structural and mechanistic similarities. Both enzymes catalyze the nonoxidative decarboxylation of 2-keto acids, yet differ considerably in their substrate specificity. In particular, the BFDC from P. putida exhibits very limited activity with pyruvate, whereas the PDCs from S. cerevisiae or from Z. mobilis show virtually no activity with benzoylformate (phenylglyoxylate). Previously, saturation mutagenesis was used to generate the BFDC T377L/A460Y variant, which exhibited a greater than 10,000-fold increase in pyruvate/benzoylformate substrate utilization ratio compared to that of wtBFDC. Much of this change could be attributed to an improvement in the Km value for pyruvate and, concomitantly, a decrease in the kcat value for benzoylformate. However, the steady-state data did not provide any details about changes in individual catalytic steps. To gain insight into the changes in conversion rates of pyruvate and benzoylformate to acetaldehyde and benzaldehyde, respectively, by the BFDC T377L/A460Y variant, reaction intermediates of both substrates were analyzed by NMR and microscopic rate constants for the elementary catalytic steps were calculated. Herein we also report the high resolution X-ray structure of the BFDC T377L/A460Y variant, which provides context for the observed changes in substrate specificity." @default.
- W2557551977 created "2016-12-08" @default.
- W2557551977 creator A5011535317 @default.
- W2557551977 creator A5019658723 @default.
- W2557551977 creator A5056823974 @default.
- W2557551977 creator A5064673349 @default.
- W2557551977 creator A5071720869 @default.
- W2557551977 creator A5075750467 @default.
- W2557551977 date "2016-11-30" @default.
- W2557551977 modified "2023-10-14" @default.
- W2557551977 title "Mechanistic and Structural Insight to an Evolved Benzoylformate Decarboxylase with Enhanced Pyruvate Decarboxylase Activity" @default.
- W2557551977 cites W1571794098 @default.
- W2557551977 cites W1577152142 @default.
- W2557551977 cites W1600317475 @default.
- W2557551977 cites W1608001440 @default.
- W2557551977 cites W1857282424 @default.
- W2557551977 cites W1965312304 @default.
- W2557551977 cites W1966038727 @default.
- W2557551977 cites W1966933263 @default.
- W2557551977 cites W1968538697 @default.
- W2557551977 cites W1969952818 @default.
- W2557551977 cites W1987083186 @default.
- W2557551977 cites W1990232883 @default.
- W2557551977 cites W1997428600 @default.
- W2557551977 cites W1998938459 @default.
- W2557551977 cites W1999659501 @default.
- W2557551977 cites W2000024111 @default.
- W2557551977 cites W2001641653 @default.
- W2557551977 cites W2003506907 @default.
- W2557551977 cites W2004835473 @default.
- W2557551977 cites W2004915899 @default.
- W2557551977 cites W2006512938 @default.
- W2557551977 cites W2011458126 @default.
- W2557551977 cites W2014117316 @default.
- W2557551977 cites W2015324008 @default.
- W2557551977 cites W2018779619 @default.
- W2557551977 cites W2024287069 @default.
- W2557551977 cites W2028635434 @default.
- W2557551977 cites W2033319848 @default.
- W2557551977 cites W2037789407 @default.
- W2557551977 cites W2040016550 @default.
- W2557551977 cites W2048511854 @default.
- W2557551977 cites W2050836805 @default.
- W2557551977 cites W2056425275 @default.
- W2557551977 cites W2068083144 @default.
- W2557551977 cites W2074136878 @default.
- W2557551977 cites W2076075565 @default.
- W2557551977 cites W2077352701 @default.
- W2557551977 cites W2082270688 @default.
- W2557551977 cites W2084082264 @default.
- W2557551977 cites W2084757892 @default.
- W2557551977 cites W2085157281 @default.
- W2557551977 cites W2090141453 @default.
- W2557551977 cites W2094026686 @default.
- W2557551977 cites W2101071612 @default.
- W2557551977 cites W2122339645 @default.
- W2557551977 cites W2123675215 @default.
- W2557551977 cites W2125576777 @default.
- W2557551977 cites W2127160311 @default.
- W2557551977 cites W2129458031 @default.
- W2557551977 cites W2132886727 @default.
- W2557551977 cites W2144081223 @default.
- W2557551977 cites W2151241493 @default.
- W2557551977 cites W2154714625 @default.
- W2557551977 cites W2180229411 @default.
- W2557551977 cites W2318134820 @default.
- W2557551977 cites W2952021364 @default.
- W2557551977 cites W3005225192 @default.
- W2557551977 cites W4293247451 @default.
- W2557551977 doi "https://doi.org/10.3390/catal6120190" @default.
- W2557551977 hasPublicationYear "2016" @default.
- W2557551977 type Work @default.
- W2557551977 sameAs 2557551977 @default.
- W2557551977 citedByCount "3" @default.
- W2557551977 countsByYear W25575519772018 @default.
- W2557551977 countsByYear W25575519772021 @default.
- W2557551977 countsByYear W25575519772023 @default.
- W2557551977 crossrefType "journal-article" @default.
- W2557551977 hasAuthorship W2557551977A5011535317 @default.
- W2557551977 hasAuthorship W2557551977A5019658723 @default.
- W2557551977 hasAuthorship W2557551977A5056823974 @default.
- W2557551977 hasAuthorship W2557551977A5064673349 @default.
- W2557551977 hasAuthorship W2557551977A5071720869 @default.
- W2557551977 hasAuthorship W2557551977A5075750467 @default.
- W2557551977 hasBestOaLocation W25575519771 @default.
- W2557551977 hasConcept C161790260 @default.
- W2557551977 hasConcept C181199279 @default.
- W2557551977 hasConcept C185592680 @default.
- W2557551977 hasConcept C18903297 @default.
- W2557551977 hasConcept C2777179333 @default.
- W2557551977 hasConcept C2777289219 @default.
- W2557551977 hasConcept C2777344606 @default.
- W2557551977 hasConcept C2777595113 @default.
- W2557551977 hasConcept C2780161600 @default.
- W2557551977 hasConcept C2780595602 @default.
- W2557551977 hasConcept C2781281423 @default.
- W2557551977 hasConcept C41183919 @default.
- W2557551977 hasConcept C55493867 @default.