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- W2557681856 abstract "Human general transcription factor TFIID is a megadalton sized multiprotein complex containing the TATA-box binding protein (TBP) and 13 TBP Associated Factors (TAFs). Structural studies by cryo-electron microscopy (cryo-EM) have uncovered the overall architecture of TFIID, providing valuable insight into subunit assembly and promoter recognition at medium resolution. Despite considerable effort, high resolution structural information of TAFs to date have been largely limited to structures of individual domains and assemblies of TAF histone-fold pairs, which are prevalent in TFIID. We used our MultiBac baculovirus/insect cell technology to obtain recombinant TFIID complexes including holo-TFIID in unprecedented quality and quantity, setting the stage for high resolution structural analysis by X-ray crystallography. We identified stable subassemblies of the TFIID complexes by limited proteolysis, and utilized a rapid combinatorial co-expression approach to produce and purify specimens for high-throughput crystallization. We crystallized a thus defined complex formed by TAF5, TAF6 and TAF9 and determined the structure to a resolution of 2.7 Å, revealing intricate molecular interactions at the core of human TFIID." @default.
- W2557681856 created "2016-12-08" @default.
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- W2557681856 date "2014-08-05" @default.
- W2557681856 modified "2023-09-25" @default.
- W2557681856 title "Crystal structure of human TAF5/TAF6/TAF9 complex at 2.7 A resolution" @default.
- W2557681856 doi "https://doi.org/10.1107/s2053273314088342" @default.
- W2557681856 hasPublicationYear "2014" @default.
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