Matches in SemOpenAlex for { <https://semopenalex.org/work/W2557737992> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2557737992 endingPage "224" @default.
- W2557737992 startingPage "218" @default.
- W2557737992 abstract "In vitro culture of neural stem cell-derived neurons serves as an excellent model to study anesthetic-induced neurotoxicity. In our study, we examined the functional role of long non-coding RNA, IGF2AS, in regulating ketamine-induced neurotoxicity in murine neural stem cells.Murine E18.5 brain-derived neural stem cells were cultured in vitro. During neural differentiation stage, ketamine-induced gene expression changes of IGF2 and IGF2AS were recorded by qRT-PCR. Neural stem cell culture was then infected by IGF2AS siRNA. The protective effect of IGF2AS inhibition on ketamine-induced apoptosis and neurite loss was assessed by TUNEL and neurite growth assays. BDNF and Akt, two candidates of downstream signaling pathways associated with IGF2AS inhibition, were further examined by western blot in neural stem cell culture.In neural stem cell culture, IGF2 was downregulated, but IGF2AS upregulated by in vitro treatment of ketamine in dose-dependent manner. Transfection of IGF2AS-specific siRNA effectively downregulated endogenous IGF2AS expression in neural stem cells. In addition, IGF2AS inhibition significantly alleviated ketamine-induced neuronal apoptosis and neurite loss in neural stem cell-derived neurons. Western blot study revealed that IGF2AS inhibition upregulated downstream pro-neuronal signaling pathway proteins BDNF and Akt.Inhibiting endogenous IGF2AS can protect anesthetic-induced neurotoxicity in neural stem cells, possibly through complimentary IGF2 upregulation and its associated downstream signaling pathways." @default.
- W2557737992 created "2016-12-08" @default.
- W2557737992 creator A5006423119 @default.
- W2557737992 creator A5024644382 @default.
- W2557737992 creator A5039646510 @default.
- W2557737992 creator A5040686568 @default.
- W2557737992 date "2017-01-01" @default.
- W2557737992 modified "2023-10-14" @default.
- W2557737992 title "Inhibition of long non-coding RNA IGF2AS protects apoptosis and neuronal loss in anesthetic-damaged mouse neural stem cell derived neurons" @default.
- W2557737992 cites W1827431288 @default.
- W2557737992 cites W1899341317 @default.
- W2557737992 cites W1987497534 @default.
- W2557737992 cites W1990895545 @default.
- W2557737992 cites W2006992053 @default.
- W2557737992 cites W2017920168 @default.
- W2557737992 cites W2038151537 @default.
- W2557737992 cites W2043574383 @default.
- W2557737992 cites W2050982262 @default.
- W2557737992 cites W2053418969 @default.
- W2557737992 cites W2055027675 @default.
- W2557737992 cites W2094105781 @default.
- W2557737992 cites W2099930837 @default.
- W2557737992 cites W2126832282 @default.
- W2557737992 cites W2154942988 @default.
- W2557737992 cites W2176555004 @default.
- W2557737992 cites W2334861214 @default.
- W2557737992 cites W2336508388 @default.
- W2557737992 cites W2396651713 @default.
- W2557737992 cites W2442318256 @default.
- W2557737992 cites W2463141156 @default.
- W2557737992 cites W4298115426 @default.
- W2557737992 cites W613446275 @default.
- W2557737992 doi "https://doi.org/10.1016/j.biopha.2016.10.094" @default.
- W2557737992 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27914827" @default.
- W2557737992 hasPublicationYear "2017" @default.
- W2557737992 type Work @default.
- W2557737992 sameAs 2557737992 @default.
- W2557737992 citedByCount "18" @default.
- W2557737992 countsByYear W25577379922017 @default.
- W2557737992 countsByYear W25577379922018 @default.
- W2557737992 countsByYear W25577379922019 @default.
- W2557737992 countsByYear W25577379922020 @default.
- W2557737992 countsByYear W25577379922021 @default.
- W2557737992 countsByYear W25577379922022 @default.
- W2557737992 crossrefType "journal-article" @default.
- W2557737992 hasAuthorship W2557737992A5006423119 @default.
- W2557737992 hasAuthorship W2557737992A5024644382 @default.
- W2557737992 hasAuthorship W2557737992A5039646510 @default.
- W2557737992 hasAuthorship W2557737992A5040686568 @default.
- W2557737992 hasConcept C113246987 @default.
- W2557737992 hasConcept C136834591 @default.
- W2557737992 hasConcept C178790620 @default.
- W2557737992 hasConcept C185592680 @default.
- W2557737992 hasConcept C202751555 @default.
- W2557737992 hasConcept C2779491297 @default.
- W2557737992 hasConcept C28328180 @default.
- W2557737992 hasConcept C29730261 @default.
- W2557737992 hasConcept C4746552 @default.
- W2557737992 hasConcept C55493867 @default.
- W2557737992 hasConcept C62478195 @default.
- W2557737992 hasConcept C75217442 @default.
- W2557737992 hasConcept C86554907 @default.
- W2557737992 hasConcept C86803240 @default.
- W2557737992 hasConcept C95444343 @default.
- W2557737992 hasConceptScore W2557737992C113246987 @default.
- W2557737992 hasConceptScore W2557737992C136834591 @default.
- W2557737992 hasConceptScore W2557737992C178790620 @default.
- W2557737992 hasConceptScore W2557737992C185592680 @default.
- W2557737992 hasConceptScore W2557737992C202751555 @default.
- W2557737992 hasConceptScore W2557737992C2779491297 @default.
- W2557737992 hasConceptScore W2557737992C28328180 @default.
- W2557737992 hasConceptScore W2557737992C29730261 @default.
- W2557737992 hasConceptScore W2557737992C4746552 @default.
- W2557737992 hasConceptScore W2557737992C55493867 @default.
- W2557737992 hasConceptScore W2557737992C62478195 @default.
- W2557737992 hasConceptScore W2557737992C75217442 @default.
- W2557737992 hasConceptScore W2557737992C86554907 @default.
- W2557737992 hasConceptScore W2557737992C86803240 @default.
- W2557737992 hasConceptScore W2557737992C95444343 @default.
- W2557737992 hasLocation W25577379921 @default.
- W2557737992 hasLocation W25577379922 @default.
- W2557737992 hasOpenAccess W2557737992 @default.
- W2557737992 hasPrimaryLocation W25577379921 @default.
- W2557737992 hasRelatedWork W2020067517 @default.
- W2557737992 hasRelatedWork W2045039713 @default.
- W2557737992 hasRelatedWork W2130946516 @default.
- W2557737992 hasRelatedWork W219266316 @default.
- W2557737992 hasRelatedWork W2460174182 @default.
- W2557737992 hasRelatedWork W2790853355 @default.
- W2557737992 hasRelatedWork W2798725455 @default.
- W2557737992 hasRelatedWork W2953204395 @default.
- W2557737992 hasRelatedWork W3004915253 @default.
- W2557737992 hasRelatedWork W3156245950 @default.
- W2557737992 hasVolume "85" @default.
- W2557737992 isParatext "false" @default.
- W2557737992 isRetracted "false" @default.
- W2557737992 magId "2557737992" @default.
- W2557737992 workType "article" @default.