Matches in SemOpenAlex for { <https://semopenalex.org/work/W2557808664> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2557808664 endingPage "3406" @default.
- W2557808664 startingPage "3399" @default.
- W2557808664 abstract "Microtubule dynamics rely on the properties of tubulin and are regulated by microtubule-associated proteins. GTP-tubulin assembles into hollow polymers, which can depolymerize upon GTP hydrolysis. Depolymerizing microtubules may stop shrinking and resume growth. Such rescues are regulated by microtubule-associated proteins like CLIP-170 and the CLASPs [1, 2]. Microtubule domains prone to rescues contain discrete regions (previously termed GTP islands) that retain a GTP-tubulin-like conformation in the main body of the microtubule [3]. However, the exact nature of these domains and the mechanisms controlling their occurrence and distribution are largely unknown. Here we show that collisions between growing microtubules and mechanical obstacles (including other microtubules) in vitro result in the higher abundance of GTP-like islands in stressed microtubule regions. Furthermore, these islands were found to be efficiently generated by both lateral contacts and mechanical constraints applied to the main body of the microtubules. They were also particularly prominent where shifts in the number of protofilaments occur in the microtubule lattice. GTP-like islands and rescues frequently co-occurred at microtubule intersections in vitro and in living cells, both in crossing and in crossed microtubules. We also observed that CLIP-170 recognizes GTP-like islands in vivo and is retained at microtubule crossings. Therefore, we propose that rescues occur via a two-stage mechanism: (1) lattice defects determine potential rescue-promoting islands in the microtubule structure, and (2) CLIP-170 detects these islands to stimulate microtubule rescue. Our results reveal the interplay between rescue-promoting factors and microtubule architecture and organization to control microtubule dynamics." @default.
- W2557808664 created "2016-12-08" @default.
- W2557808664 creator A5000718357 @default.
- W2557808664 creator A5038388507 @default.
- W2557808664 creator A5052350307 @default.
- W2557808664 creator A5073638203 @default.
- W2557808664 creator A5076182858 @default.
- W2557808664 creator A5085364478 @default.
- W2557808664 creator A5091013056 @default.
- W2557808664 date "2016-12-01" @default.
- W2557808664 modified "2023-10-11" @default.
- W2557808664 title "Localized Mechanical Stress Promotes Microtubule Rescue" @default.
- W2557808664 cites W1803436399 @default.
- W2557808664 cites W1977403588 @default.
- W2557808664 cites W2001859337 @default.
- W2557808664 cites W2021135236 @default.
- W2557808664 cites W2022288724 @default.
- W2557808664 cites W2022938224 @default.
- W2557808664 cites W2027758338 @default.
- W2557808664 cites W2039707521 @default.
- W2557808664 cites W2039737272 @default.
- W2557808664 cites W2041177122 @default.
- W2557808664 cites W2085290831 @default.
- W2557808664 cites W2089843126 @default.
- W2557808664 cites W2100343024 @default.
- W2557808664 cites W2129053861 @default.
- W2557808664 cites W2154968967 @default.
- W2557808664 cites W2159335259 @default.
- W2557808664 cites W2404925385 @default.
- W2557808664 cites W2519267297 @default.
- W2557808664 doi "https://doi.org/10.1016/j.cub.2016.10.048" @default.
- W2557808664 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27916523" @default.
- W2557808664 hasPublicationYear "2016" @default.
- W2557808664 type Work @default.
- W2557808664 sameAs 2557808664 @default.
- W2557808664 citedByCount "65" @default.
- W2557808664 countsByYear W25578086642016 @default.
- W2557808664 countsByYear W25578086642017 @default.
- W2557808664 countsByYear W25578086642018 @default.
- W2557808664 countsByYear W25578086642019 @default.
- W2557808664 countsByYear W25578086642020 @default.
- W2557808664 countsByYear W25578086642021 @default.
- W2557808664 countsByYear W25578086642022 @default.
- W2557808664 countsByYear W25578086642023 @default.
- W2557808664 crossrefType "journal-article" @default.
- W2557808664 hasAuthorship W2557808664A5000718357 @default.
- W2557808664 hasAuthorship W2557808664A5038388507 @default.
- W2557808664 hasAuthorship W2557808664A5052350307 @default.
- W2557808664 hasAuthorship W2557808664A5073638203 @default.
- W2557808664 hasAuthorship W2557808664A5076182858 @default.
- W2557808664 hasAuthorship W2557808664A5085364478 @default.
- W2557808664 hasAuthorship W2557808664A5091013056 @default.
- W2557808664 hasBestOaLocation W25578086641 @default.
- W2557808664 hasConcept C118716 @default.
- W2557808664 hasConcept C181199279 @default.
- W2557808664 hasConcept C20418707 @default.
- W2557808664 hasConcept C55493867 @default.
- W2557808664 hasConcept C61081601 @default.
- W2557808664 hasConcept C84425145 @default.
- W2557808664 hasConcept C86803240 @default.
- W2557808664 hasConcept C95444343 @default.
- W2557808664 hasConceptScore W2557808664C118716 @default.
- W2557808664 hasConceptScore W2557808664C181199279 @default.
- W2557808664 hasConceptScore W2557808664C20418707 @default.
- W2557808664 hasConceptScore W2557808664C55493867 @default.
- W2557808664 hasConceptScore W2557808664C61081601 @default.
- W2557808664 hasConceptScore W2557808664C84425145 @default.
- W2557808664 hasConceptScore W2557808664C86803240 @default.
- W2557808664 hasConceptScore W2557808664C95444343 @default.
- W2557808664 hasFunder F4320320883 @default.
- W2557808664 hasFunder F4320320893 @default.
- W2557808664 hasFunder F4320321589 @default.
- W2557808664 hasFunder F4320322361 @default.
- W2557808664 hasFunder F4320322890 @default.
- W2557808664 hasFunder F4320322892 @default.
- W2557808664 hasFunder F4320326454 @default.
- W2557808664 hasIssue "24" @default.
- W2557808664 hasLocation W25578086641 @default.
- W2557808664 hasLocation W25578086642 @default.
- W2557808664 hasLocation W25578086643 @default.
- W2557808664 hasLocation W25578086644 @default.
- W2557808664 hasOpenAccess W2557808664 @default.
- W2557808664 hasPrimaryLocation W25578086641 @default.
- W2557808664 hasRelatedWork W1901412126 @default.
- W2557808664 hasRelatedWork W1974089612 @default.
- W2557808664 hasRelatedWork W2022882400 @default.
- W2557808664 hasRelatedWork W2024145976 @default.
- W2557808664 hasRelatedWork W2031263977 @default.
- W2557808664 hasRelatedWork W2070191397 @default.
- W2557808664 hasRelatedWork W2079016385 @default.
- W2557808664 hasRelatedWork W2089843126 @default.
- W2557808664 hasRelatedWork W2415327659 @default.
- W2557808664 hasRelatedWork W2901583425 @default.
- W2557808664 hasVolume "26" @default.
- W2557808664 isParatext "false" @default.
- W2557808664 isRetracted "false" @default.
- W2557808664 magId "2557808664" @default.
- W2557808664 workType "article" @default.