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W2558551486 abstract "Abstract Quantitative and kinetic analyses of apoptotic cell death are integral components of exploring cell biology, measuring cellular stress responses, and performing high-throughput genomic/RNAi/drug screens. Here, we present a detailed method that integrates robust kinetic real-time high-content imaging with Annexin V labelling to provide a highly sensitive, accurate, simple and zero-handling approach to quantify extrinsic and intrinsic inducers of apoptosis. The sensitivity of this non-toxic method outperforms previous high-throughput methodologies using viability dyes or caspase-activation reporters. This method also incorporates a multiplex adaptation to integrate variability in cell number due to treatment-induced proliferation changes and the detachment of dying cells. Compared to Annexin V detection by flow cytometry, this method is 10-fold more sensitive, eliminates extensive sample handling and processing, and provides real-time kinetics of apoptosis at both single-cell and population-level resolutions." @default.
W2558551486 created "2016-12-08" @default.
W2558551486 creator A5016580341 @default.
W2558551486 creator A5061440261 @default.
W2558551486 date "2016-12-01" @default.
W2558551486 modified "2023-10-06" @default.
W2558551486 title "Robust high-throughput kinetic analysis of apoptosis with real-time high-content live-cell imaging" @default.
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W2558551486 doi "https://doi.org/10.1038/cddis.2016.332" @default.
W2558551486 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5520694" @default.
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