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• W2558902471 abstract "Employing biophysical and structural methods is a powerful way to elucidate mechanisms of molecular recognition in bacterial pathogenesis. Such studies invariably depend on the production of pure, folded and stable proteins. Many proteins that can be expressed recombinantly ultimately fail to meet one or more of these criteria. The cag proteins from Helicobacter pylori form a secretion system that delivers the oncoprotein, CagA, into human gastric epithelial cells through an interaction between CagL and host cell integrins, where it can cause gastric adenocarcinoma. Expression of full length CagA and CagL is problematic as CagA undergoes rapid degradation during purification and CagL is thermally unstable. Here, we describe a method for the purification of CagA that results in the production of the full length protein through coexpression with its endogenous chaperone, CagF, and its subsequent separation from its chaperone. Furthermore, we detail the production of CagL and the use of differential scanning fluorimetry to identify how CagL is thermally stabilized by reduced pH, which led to a new crystal form of CagL and novel insight to pathogenic mechanisms. The methods described here for the production of stable cag proteins can be applied to a wide range of proteins involved in bacterial pathogenesis." @default.
• W2558902471 created "2016-12-08" @default.
• W2558902471 creator A5037693404 @default.
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• W2558902471 date "2016-12-03" @default.
• W2558902471 modified "2023-09-23" @default.
• W2558902471 title "Common Challenges in Studying the Structure and Function of Bacterial Proteins: Case Studies from Helicobacter pylori" @default.
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• W2558902471 doi "https://doi.org/10.1007/978-1-4939-6673-8_6" @default.
• W2558902471 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27914074" @default.
• W2558902471 hasPublicationYear "2016" @default.
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