FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2559084811> ?p ?o ?g. }

• W2559084811 endingPage "377" @default.
• W2559084811 startingPage "364" @default.
• W2559084811 abstract "In this study, we report how different cell disruption methods, PCR primers and in silico analyses can seriously bias results from microbial population studies, with consequences for the credibility and reproducibility of the findings. Our results emphasize the pitfalls of commonly used experimental methods that can seriously weaken the interpretation of results.Four different cell lysis methods, three commonly used primer pairs and various computer-based analyses were applied to investigate the microbial diversity of a fermentation sample composed of chicken dung. The fault-prone, but still frequently used, amplified rRNA gene restriction analysis was chosen to identify common weaknesses. In contrast to other studies, we focused on the complete analytical process, from cell disruption to in silico analysis, and identified potential error rates. This identified a wide disagreement of results between applied experimental approaches leading to very different community structures depending on the chosen approach.The interpretation of microbial diversity data remains a challenge. In order to accurately investigate the taxonomic diversity and structure of prokaryotic communities, we suggest a multi-level approach combining DNA-based and DNA-independent techniques.The identified weaknesses of commonly used methods to study microbial diversity can be overcome by a multi-level approach, which produces more reliable data about the fate and behaviour of microbial communities of engineered habitats such as biogas plants, so that the best performance can be ensured." @default.
• W2559084811 created "2016-12-08" @default.
• W2559084811 creator A5011609494 @default.
• W2559084811 creator A5019452377 @default.
• W2559084811 creator A5026425746 @default.
• W2559084811 creator A5069495933 @default.
• W2559084811 date "2017-01-13" @default.
• W2559084811 modified "2023-09-25" @default.
• W2559084811 title "Methodological flaws introduce strong bias into molecular analysis of microbial populations" @default.
• W2559084811 cites W1514657191 @default.
• W2559084811 cites W1517821097 @default.
• W2559084811 cites W1582413449 @default.
• W2559084811 cites W1966437538 @default.
• W2559084811 cites W1968792443 @default.
• W2559084811 cites W1973881305 @default.
• W2559084811 cites W1979343540 @default.
• W2559084811 cites W1984202841 @default.
• W2559084811 cites W1987336530 @default.
• W2559084811 cites W1996738992 @default.
• W2559084811 cites W2015068088 @default.
• W2559084811 cites W2038181471 @default.
• W2559084811 cites W2057876155 @default.
• W2559084811 cites W2065775222 @default.
• W2559084811 cites W2065838202 @default.
• W2559084811 cites W2070890577 @default.
• W2559084811 cites W2071481736 @default.
• W2559084811 cites W2072252388 @default.
• W2559084811 cites W2089196290 @default.
• W2559084811 cites W2102604521 @default.
• W2559084811 cites W2103740237 @default.
• W2559084811 cites W2107974372 @default.
• W2559084811 cites W2113606293 @default.
• W2559084811 cites W2114392707 @default.
• W2559084811 cites W2115631166 @default.
• W2559084811 cites W2118537438 @default.
• W2559084811 cites W2119686278 @default.
• W2559084811 cites W2140319224 @default.
• W2559084811 cites W2142894759 @default.
• W2559084811 cites W2146209885 @default.
• W2559084811 cites W2152207030 @default.
• W2559084811 cites W2152825437 @default.
• W2559084811 cites W2152885278 @default.
• W2559084811 cites W2153823136 @default.
• W2559084811 cites W2154401636 @default.
• W2559084811 cites W2155806125 @default.
• W2559084811 cites W2156232632 @default.
• W2559084811 cites W2159068661 @default.
• W2559084811 cites W2159480438 @default.
• W2559084811 cites W2170052798 @default.
• W2559084811 doi "https://doi.org/10.1111/jam.13365" @default.
• W2559084811 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27914209" @default.
• W2559084811 hasPublicationYear "2017" @default.
• W2559084811 type Work @default.
• W2559084811 sameAs 2559084811 @default.
• W2559084811 citedByCount "13" @default.
• W2559084811 countsByYear W25590848112017 @default.
• W2559084811 countsByYear W25590848112018 @default.
• W2559084811 countsByYear W25590848112019 @default.
• W2559084811 countsByYear W25590848112020 @default.
• W2559084811 countsByYear W25590848112021 @default.
• W2559084811 countsByYear W25590848112022 @default.
• W2559084811 countsByYear W25590848112023 @default.
• W2559084811 crossrefType "journal-article" @default.
• W2559084811 hasAuthorship W2559084811A5011609494 @default.
• W2559084811 hasAuthorship W2559084811A5019452377 @default.
• W2559084811 hasAuthorship W2559084811A5026425746 @default.
• W2559084811 hasAuthorship W2559084811A5069495933 @default.
• W2559084811 hasBestOaLocation W25590848111 @default.
• W2559084811 hasConcept C104317684 @default.
• W2559084811 hasConcept C127413603 @default.
• W2559084811 hasConcept C144024400 @default.
• W2559084811 hasConcept C149923435 @default.
• W2559084811 hasConcept C150903083 @default.
• W2559084811 hasConcept C154945302 @default.
• W2559084811 hasConcept C183696295 @default.
• W2559084811 hasConcept C186060115 @default.
• W2559084811 hasConcept C2775905019 @default.
• W2559084811 hasConcept C2908647359 @default.
• W2559084811 hasConcept C41008148 @default.
• W2559084811 hasConcept C54355233 @default.
• W2559084811 hasConcept C70721500 @default.
• W2559084811 hasConcept C79337645 @default.
• W2559084811 hasConcept C86803240 @default.
• W2559084811 hasConceptScore W2559084811C104317684 @default.
• W2559084811 hasConceptScore W2559084811C127413603 @default.
• W2559084811 hasConceptScore W2559084811C144024400 @default.
• W2559084811 hasConceptScore W2559084811C149923435 @default.
• W2559084811 hasConceptScore W2559084811C150903083 @default.
• W2559084811 hasConceptScore W2559084811C154945302 @default.
• W2559084811 hasConceptScore W2559084811C183696295 @default.
• W2559084811 hasConceptScore W2559084811C186060115 @default.
• W2559084811 hasConceptScore W2559084811C2775905019 @default.
• W2559084811 hasConceptScore W2559084811C2908647359 @default.
• W2559084811 hasConceptScore W2559084811C41008148 @default.
• W2559084811 hasConceptScore W2559084811C54355233 @default.
• W2559084811 hasConceptScore W2559084811C70721500 @default.
• W2559084811 hasConceptScore W2559084811C79337645 @default.
• W2559084811 hasConceptScore W2559084811C86803240 @default.

• next