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- W2559172502 abstract "Patient samples: Study was approved by the institutional review board (14-009025). Plasma samples were stored at -80 degrees before use. Tissues were requested from the pathology research core. Galectin-9 concentration: Soluble galectin-9 was measured using enzyme linked immunosorbant assays (ELISA). Tissue staining: FFPE healthy placentas and metastatic melanoma tumors were sectioned and immunohistochemistry (IHC) stained for PDL1 and galectin-9 expression. Slides were visualized using IHCscore. Survival curve: Cutoff Finder® was utilized to determine the appropriate cutoff value between high and low galectin-9 patients. ROC found 1553pg/mL resulted in a 82% sensitivity and specificity with the AUC being 0.9. Overall survival from date of blood draw (prior to onset of therapy) was reported. Mixed lymphocyte reaction: Average and standard deviation of three independent autologous MLR using PBMCs isolated from healthy donors with or without exogenous galectin-9 (2ug/mL). Flow cytometry: PBMC subsets were measured after a 24 hour incubation with galectin-9 (2ug/mL) using a Guava easyCyte 8HT flow cytometer. T helper cells (CD3+CD4+), T helper 1 cells (CD3+CD4+TIM3+), monocytes (CD14+), activated dendritic cells (CD11c+CD86+) and natural killer cells (CD16+CD56+) were measured. Methods The aims of this research include:" @default.
- W2559172502 created "2016-12-08" @default.
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- W2559172502 date "2015-01-01" @default.
- W2559172502 modified "2023-09-26" @default.
- W2559172502 title "The role of galectin-9 in metastatic melanoma and pregnancy, and its impact on outcome" @default.
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