FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2559957105> ?p ?o ?g. }

• W2559957105 endingPage "476" @default.
• W2559957105 startingPage "469" @default.
• W2559957105 abstract "Objective This report presents tenofovir (TFV) alafenamide (TAF) and elvitegravir (EVG) fabricated into nanoparticles for subcutaneous delivery as prevention strategy. Design Prospective prevention study in humanized bone marrow-liver-thymus (hu-BLT) mice. Methods Using an oil-in-water emulsion solvent evaporation technique, TAF + EVG drugs were entrapped together into nanoparticles containing poly(lactic-co-glycolic acid). In-vitro prophylaxis studies (90% inhibition concentration) compared nanoparticles with drugs in solution. Hu-BLT (n = 5/group) mice were given 200 mg/kg subcutaneous, and vaginally challenged with HIV-1 [5 × 10 tissue culture infectious dose for 50% of cells cultures (TCID50)] 4 and 14 days post-nanoparticle administration (post-nanoparticle injection). Control mice (n = 5) were challenged at 4 days. Weekly plasma viral load was performed using RT-PCR. Hu-BLT mice were sacrificed and lymph nodes were harvested for HIV-1 viral RNA detection by in-situ hybridization. In parallel, CD34 humanized mice (3/time point) compared TFV and EVG drug levels in vaginal tissues from nanoparticles and solution. TFV and EVG were analyzed from tissue using liquid chromatograph-tandem mass spectrometry (LC-MS/MS). Results TAF + EVG nanoparticles were less than 200 nm in size. In-vitro prophylaxis indicates TAF + EVG nanoparticles 90% inhibition concentration was 0.002 μg/ml and TAF + EVG solution was 0.78 μg/ml. TAF + EVG nanoparticles demonstrated detectable drugs for 14 days and 72 h for solution, respectively. All hu-BLT control mice became infected within 14 days after HIV-1 challenge. In contrast, hu-BLT mice that received nanoparticles and challenged at 4 days post-nanoparticle injection, 100% were uninfected, and 60% challenged at 14 days post-nanoparticle injection were uninfected (P = 0.007; Mantel-Cox test). In-situ hybridization confirmed these results. Conclusion This proof-of-concept study demonstrated sustained protection for TAF + EVG nanoparticles in a hu-BLT mouse model of HIV vaginal transmission." @default.
• W2559957105 created "2016-12-16" @default.
• W2559957105 creator A5002869957 @default.
• W2559957105 creator A5003445982 @default.
• W2559957105 creator A5015943195 @default.
• W2559957105 creator A5021127106 @default.
• W2559957105 creator A5030005257 @default.
• W2559957105 creator A5031015958 @default.
• W2559957105 creator A5059924135 @default.
• W2559957105 creator A5077724384 @default.
• W2559957105 date "2017-02-20" @default.
• W2559957105 modified "2023-09-26" @default.
• W2559957105 title "Tenofovir alafenamide and elvitegravir loaded nanoparticles for long-acting prevention of HIV-1 vaginal transmission" @default.
• W2559957105 cites W1592628801 @default.
• W2559957105 cites W1969274526 @default.
• W2559957105 cites W1978934985 @default.
• W2559957105 cites W1979325857 @default.
• W2559957105 cites W1981362794 @default.
• W2559957105 cites W1999396723 @default.
• W2559957105 cites W2000761749 @default.
• W2559957105 cites W2004729106 @default.
• W2559957105 cites W2014725234 @default.
• W2559957105 cites W2029654824 @default.
• W2559957105 cites W2044369525 @default.
• W2559957105 cites W2076674812 @default.
• W2559957105 cites W2095844669 @default.
• W2559957105 cites W2103920597 @default.
• W2559957105 cites W2117057469 @default.
• W2559957105 cites W2118030927 @default.
• W2559957105 cites W2134966461 @default.
• W2559957105 cites W2143055379 @default.
• W2559957105 cites W2144000274 @default.
• W2559957105 cites W2145584716 @default.
• W2559957105 cites W2162226934 @default.
• W2559957105 cites W2168251450 @default.
• W2559957105 cites W2172212902 @default.
• W2559957105 cites W2319917590 @default.
• W2559957105 cites W2335805396 @default.
• W2559957105 cites W2502542881 @default.
• W2559957105 doi "https://doi.org/10.1097/qad.0000000000001349" @default.
• W2559957105 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5283613" @default.
• W2559957105 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28121666" @default.
• W2559957105 hasPublicationYear "2017" @default.
• W2559957105 type Work @default.
• W2559957105 sameAs 2559957105 @default.
• W2559957105 citedByCount "44" @default.
• W2559957105 countsByYear W25599571052017 @default.
• W2559957105 countsByYear W25599571052018 @default.
• W2559957105 countsByYear W25599571052019 @default.
• W2559957105 countsByYear W25599571052020 @default.
• W2559957105 countsByYear W25599571052021 @default.
• W2559957105 countsByYear W25599571052022 @default.
• W2559957105 countsByYear W25599571052023 @default.
• W2559957105 crossrefType "journal-article" @default.
• W2559957105 hasAuthorship W2559957105A5002869957 @default.
• W2559957105 hasAuthorship W2559957105A5003445982 @default.
• W2559957105 hasAuthorship W2559957105A5015943195 @default.
• W2559957105 hasAuthorship W2559957105A5021127106 @default.
• W2559957105 hasAuthorship W2559957105A5030005257 @default.
• W2559957105 hasAuthorship W2559957105A5031015958 @default.
• W2559957105 hasAuthorship W2559957105A5059924135 @default.
• W2559957105 hasAuthorship W2559957105A5077724384 @default.
• W2559957105 hasBestOaLocation W25599571052 @default.
• W2559957105 hasConcept C142462285 @default.
• W2559957105 hasConcept C159047783 @default.
• W2559957105 hasConcept C2777068322 @default.
• W2559957105 hasConcept C2780524789 @default.
• W2559957105 hasConcept C2911190787 @default.
• W2559957105 hasConcept C2993143319 @default.
• W2559957105 hasConcept C3013748606 @default.
• W2559957105 hasConcept C71924100 @default.
• W2559957105 hasConceptScore W2559957105C142462285 @default.
• W2559957105 hasConceptScore W2559957105C159047783 @default.
• W2559957105 hasConceptScore W2559957105C2777068322 @default.
• W2559957105 hasConceptScore W2559957105C2780524789 @default.
• W2559957105 hasConceptScore W2559957105C2911190787 @default.
• W2559957105 hasConceptScore W2559957105C2993143319 @default.
• W2559957105 hasConceptScore W2559957105C3013748606 @default.
• W2559957105 hasConceptScore W2559957105C71924100 @default.
• W2559957105 hasIssue "4" @default.
• W2559957105 hasLocation W25599571051 @default.
• W2559957105 hasLocation W25599571052 @default.
• W2559957105 hasLocation W25599571053 @default.
• W2559957105 hasOpenAccess W2559957105 @default.
• W2559957105 hasPrimaryLocation W25599571051 @default.
• W2559957105 hasRelatedWork W2264012645 @default.
• W2559957105 hasRelatedWork W2320601665 @default.
• W2559957105 hasRelatedWork W2530847808 @default.
• W2559957105 hasRelatedWork W2553949721 @default.
• W2559957105 hasRelatedWork W2577777925 @default.
• W2559957105 hasRelatedWork W2617130959 @default.
• W2559957105 hasRelatedWork W2795554334 @default.
• W2559957105 hasRelatedWork W2937055384 @default.
• W2559957105 hasRelatedWork W3017099993 @default.
• W2559957105 hasRelatedWork W3130587537 @default.
• W2559957105 hasVolume "31" @default.
• W2559957105 isParatext "false" @default.
• W2559957105 isRetracted "false" @default.

• next