FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2559968280> ?p ?o ?g. }

• W2559968280 abstract "Abstract Abstract 1179 Bone marrow stromal cell (BMSC) therapy is effective for treating acute-Graft versus Host Disease (aGVHD) and is being investigated as a therapy for graft failure and organ failure following allogeneic hematopoietic stem cell transplantation. Clinical BMSC products are typically produced by serial passage of adherent cells obtained from marrow aspirates. Multiple passages allow for the production of large quantities of human BMSCs, but BMSC expansion is limited by cell senescence. We assessed the nature of BMSC changes associated with multiple passages in order to better understand the nature and impact of senescence on human BMSCs and to identify potential senescence associated biomarkers. BMSCs from marrow aspirates of 5 healthy subjects were cultured in flasks and passed serially until cell growth stopped between the 8th to 12th passages. Early and late passage cells were compared. The early passage BMSCs were passages 1 and 2; because the onset of senescence as assessed by growth characteristics and morphology varied among the 5 donors, the passages included in the late passage group were variable and ranged from passage 5 to 11. The samples (25 total; 10 early and 15 late) were selected based on the replicative lifespan. Senescence was associated with a change to a flattened morphology and increased senescence associated beta-galactosidase (SA β-gal) staining. Early and late passage BMSCs from each subject were also analyzed by flow cytometry and global transcriptome analysis with an oligonucleotide microarray with >44,000 probes. Unsupervised hierarchical clustering analysis separated the 25 BMSC samples into two clusters, one with all early passage and a second one with all late passage samples. A total of 1,739 genes were differentially expressed between the early and late passage BMSCs. Ingenuity pathway analysis of the differentially expressed genes found that those highly expressed in the early passage cells belonged to several immune pathways: cell-mediated immune response, humoral immune responses, hypersensitive responses, and lymphoid tissue structure and development. Genes highly expressed in the late passage BMSCs belonged to the DNA replicative, recombination and repair and nucleic acid metabolism pathways. Specific genes highly expressed in early passage BMSCs compared to late passage BMSCs included the chemokine receptor CXCR7, Secreted frizzle-related protein 4 (SFRP4), WNT1 induced signaling pathway protein 1 (WISP1), lymphocyte-specific protein 1 (LSP1), and insulin-like growth factor 1 (IGF1). Genes up-regulated in late passage cells included the thrombospondin receptor (CD36), coagulation factor III (F3), and tissue plasminogen activator (PLAT). Among the differentially expressed genes were 347 involved with the cell cycle and apoptosis. Hierarchical clustering analysis of these 347 genes again separated the early and late passage cells into two groups and a node of genes more highly expressed in late passages cells included two genes involved with replication senescence, cyclin-dependent kinase inhibitor 1A (p21, Cip1) (CDKN1A) and cyclin-dependent kinase inhibitor 2A (melanoma, p16, inhibits CDK4) (CDKN2A) and one that inhibits TRALI mediated apoptosis, tumor necrosis factor receptor superfamily, member 10d (TNFRSF10D, DCR2, CD264). Flow cytometry analysis confirmed greater expression of TNFRS10D on late passage cells. BMSC senescence is associated with distinct molecular changes. Early passage BMSCs may have more immune modulatory properties. Further studies are needed to determine more precisely the onset of senescence-associated changes. Disclosures: No relevant conflicts of interest to declare." @default.
• W2559968280 created "2016-12-16" @default.
• W2559968280 creator A5026735577 @default.
• W2559968280 creator A5027181492 @default.
• W2559968280 creator A5029598737 @default.
• W2559968280 creator A5037476049 @default.
• W2559968280 creator A5046543021 @default.
• W2559968280 creator A5051298107 @default.
• W2559968280 creator A5056190774 @default.
• W2559968280 creator A5072166479 @default.
• W2559968280 creator A5086839135 @default.
• W2559968280 creator A5091567640 @default.
• W2559968280 date "2010-11-19" @default.
• W2559968280 modified "2023-10-01" @default.
• W2559968280 title "Tracking Senescence In Human Bone Marrow Stromal Cell (BMSC) Cultures." @default.
• W2559968280 doi "https://doi.org/10.1182/blood.v116.21.1179.1179" @default.
• W2559968280 hasPublicationYear "2010" @default.
• W2559968280 type Work @default.
• W2559968280 sameAs 2559968280 @default.
• W2559968280 citedByCount "0" @default.
• W2559968280 crossrefType "journal-article" @default.
• W2559968280 hasAuthorship W2559968280A5026735577 @default.
• W2559968280 hasAuthorship W2559968280A5027181492 @default.
• W2559968280 hasAuthorship W2559968280A5029598737 @default.
• W2559968280 hasAuthorship W2559968280A5037476049 @default.
• W2559968280 hasAuthorship W2559968280A5046543021 @default.
• W2559968280 hasAuthorship W2559968280A5051298107 @default.
• W2559968280 hasAuthorship W2559968280A5056190774 @default.
• W2559968280 hasAuthorship W2559968280A5072166479 @default.
• W2559968280 hasAuthorship W2559968280A5086839135 @default.
• W2559968280 hasAuthorship W2559968280A5091567640 @default.
• W2559968280 hasConcept C104317684 @default.
• W2559968280 hasConcept C109159458 @default.
• W2559968280 hasConcept C142724271 @default.
• W2559968280 hasConcept C1491633281 @default.
• W2559968280 hasConcept C150194340 @default.
• W2559968280 hasConcept C162317418 @default.
• W2559968280 hasConcept C16685009 @default.
• W2559968280 hasConcept C16930146 @default.
• W2559968280 hasConcept C203014093 @default.
• W2559968280 hasConcept C2780007613 @default.
• W2559968280 hasConcept C28328180 @default.
• W2559968280 hasConcept C502942594 @default.
• W2559968280 hasConcept C522857546 @default.
• W2559968280 hasConcept C54355233 @default.
• W2559968280 hasConcept C553184892 @default.
• W2559968280 hasConcept C71924100 @default.
• W2559968280 hasConcept C86803240 @default.
• W2559968280 hasConcept C95444343 @default.
• W2559968280 hasConceptScore W2559968280C104317684 @default.
• W2559968280 hasConceptScore W2559968280C109159458 @default.
• W2559968280 hasConceptScore W2559968280C142724271 @default.
• W2559968280 hasConceptScore W2559968280C1491633281 @default.
• W2559968280 hasConceptScore W2559968280C150194340 @default.
• W2559968280 hasConceptScore W2559968280C162317418 @default.
• W2559968280 hasConceptScore W2559968280C16685009 @default.
• W2559968280 hasConceptScore W2559968280C16930146 @default.
• W2559968280 hasConceptScore W2559968280C203014093 @default.
• W2559968280 hasConceptScore W2559968280C2780007613 @default.
• W2559968280 hasConceptScore W2559968280C28328180 @default.
• W2559968280 hasConceptScore W2559968280C502942594 @default.
• W2559968280 hasConceptScore W2559968280C522857546 @default.
• W2559968280 hasConceptScore W2559968280C54355233 @default.
• W2559968280 hasConceptScore W2559968280C553184892 @default.
• W2559968280 hasConceptScore W2559968280C71924100 @default.
• W2559968280 hasConceptScore W2559968280C86803240 @default.
• W2559968280 hasConceptScore W2559968280C95444343 @default.
• W2559968280 hasLocation W25599682801 @default.
• W2559968280 hasOpenAccess W2559968280 @default.
• W2559968280 hasPrimaryLocation W25599682801 @default.
• W2559968280 hasRelatedWork W1817255302 @default.
• W2559968280 hasRelatedWork W1979629425 @default.
• W2559968280 hasRelatedWork W1999013362 @default.
• W2559968280 hasRelatedWork W2081876727 @default.
• W2559968280 hasRelatedWork W2116670910 @default.
• W2559968280 hasRelatedWork W2402021545 @default.
• W2559968280 hasRelatedWork W2465322459 @default.
• W2559968280 hasRelatedWork W2519039180 @default.
• W2559968280 hasRelatedWork W2551556949 @default.
• W2559968280 hasRelatedWork W2556582173 @default.
• W2559968280 hasRelatedWork W2559556560 @default.
• W2559968280 hasRelatedWork W2560293782 @default.
• W2559968280 hasRelatedWork W2589184305 @default.
• W2559968280 hasRelatedWork W2595988833 @default.
• W2559968280 hasRelatedWork W2607333727 @default.
• W2559968280 hasRelatedWork W2892367700 @default.
• W2559968280 hasRelatedWork W2980064232 @default.
• W2559968280 hasRelatedWork W2988723003 @default.
• W2559968280 hasRelatedWork W3117746849 @default.
• W2559968280 hasRelatedWork W79768257 @default.
• W2559968280 isParatext "false" @default.
• W2559968280 isRetracted "false" @default.
• W2559968280 magId "2559968280" @default.
• W2559968280 workType "article" @default.