FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2560147335> ?p ?o ?g. }

• W2560147335 endingPage "2802" @default.
• W2560147335 startingPage "2802" @default.
• W2560147335 abstract "Abstract Epigenetic silencing of tumor suppressor (TS) genes is a hallmark in human leukemias, particularly through DNA methylation. Cyclin-dependent kinase inhibitors (CKI) are, among other genes, frequently found methylated in their promoter region. This epigenetic modification has been described also in acute lymphoblastic leukemia (ALL). However, the relationship between aberrant DNA methylation and protein expression of TS genes has not yet been extensively evaluated in adult ALL series. The aim of this study was to analyze in primary cells from newly diagnosed adult ALL patients, uniformly treated according to the LAL2000 GIMEMA protocol, the promoter methylation status of p73, p21, p15 and p16, evaluating in addition the p21, p15 and p16 protein expression. The DNA methylation status of promoter regions was investigated, according to cell availability, using a widely accepted method based on bisulfite modification of DNA, followed by methylation-specific PCR assay (MSP). Protein expression was evaluated by Western blot. Normal peripheral blood lymphocytes, as already described, resulted unmethylated for p73, p21, p15 and p16, and did not express the p21, p15 and p16 proteins. In ALL patients, in contrast, only the p21 promoter region was found constantly unmethylated. The p15, p16 and p73 promoter genes were found methylated in 15/37 (40.5%), 8/43 (18.6%) and 9/36 (25%) patients, respectively. Only 2/23 cases (8.6%) resulted simultaneously methylated for p15, p16 and p73. The p21 and p15 protein expression was found in 28/85 (32.9%) and 44/85 cases (51.8%), respectively. The p16 protein, in contrast, was never expressed. The p16 methylation was associated with the T-ALL (P=0.005) phenotype and with higher white blood cell (WBC) counts (P=0.027). Resistance to spontaneous induction of apoptosis was significantly associated with p21 protein expression (P=0.019) and its co-expression with p15 (P=0.049). Achievement of CR was not influenced by gene methylation status, nor by single protein expression. Interestingly, the co-expression of p15 and p21 was associated with failure to induction treatment: only 6/63 (9.5%) patients co-expressing p15 and p21 obtained a CR (P=0.027). Multivariate analysis confirmed the unfavorable role of this protein co-expression (P=0.059) on CR achievement. In contrast, once patients achieved remission, p21 protein expression was associated with a prolonged DFS, as confirmed by multivariate analysis for DFS (P=0.039). In conclusion, p15 and p21 protein expression plays an unfavorable prognostic role in adult ALL patients independently of the p73, p21, p15 and p16 gene promoter methylation status." @default.
• W2560147335 created "2016-12-16" @default.
• W2560147335 creator A5003274863 @default.
• W2560147335 creator A5016603192 @default.
• W2560147335 creator A5016635620 @default.
• W2560147335 creator A5016826234 @default.
• W2560147335 creator A5020789491 @default.
• W2560147335 creator A5021908105 @default.
• W2560147335 creator A5023749417 @default.
• W2560147335 creator A5025347398 @default.
• W2560147335 creator A5030922818 @default.
• W2560147335 creator A5031937532 @default.
• W2560147335 creator A5034393745 @default.
• W2560147335 creator A5036246648 @default.
• W2560147335 creator A5036753990 @default.
• W2560147335 creator A5039757859 @default.
• W2560147335 creator A5042609656 @default.
• W2560147335 creator A5045814742 @default.
• W2560147335 creator A5046536404 @default.
• W2560147335 creator A5057593886 @default.
• W2560147335 creator A5058459820 @default.
• W2560147335 creator A5068687462 @default.
• W2560147335 creator A5069218034 @default.
• W2560147335 creator A5070129134 @default.
• W2560147335 creator A5070417705 @default.
• W2560147335 creator A5072698345 @default.
• W2560147335 creator A5074331393 @default.
• W2560147335 creator A5077721366 @default.
• W2560147335 creator A5078192708 @default.
• W2560147335 date "2007-11-16" @default.
• W2560147335 modified "2023-10-15" @default.
• W2560147335 title "Protein Expression of p15 and p21 Plays an Unfavorable Prognostic Role in Adult Acute Lymphoblastic Leukemia (ALL) Patients Independently of Their Gene Promoter Methylation Status." @default.
• W2560147335 doi "https://doi.org/10.1182/blood.v110.11.2802.2802" @default.
• W2560147335 hasPublicationYear "2007" @default.
• W2560147335 type Work @default.
• W2560147335 sameAs 2560147335 @default.
• W2560147335 citedByCount "2" @default.
• W2560147335 countsByYear W25601473352022 @default.
• W2560147335 crossrefType "journal-article" @default.
• W2560147335 hasAuthorship W2560147335A5003274863 @default.
• W2560147335 hasAuthorship W2560147335A5016603192 @default.
• W2560147335 hasAuthorship W2560147335A5016635620 @default.
• W2560147335 hasAuthorship W2560147335A5016826234 @default.
• W2560147335 hasAuthorship W2560147335A5020789491 @default.
• W2560147335 hasAuthorship W2560147335A5021908105 @default.
• W2560147335 hasAuthorship W2560147335A5023749417 @default.
• W2560147335 hasAuthorship W2560147335A5025347398 @default.
• W2560147335 hasAuthorship W2560147335A5030922818 @default.
• W2560147335 hasAuthorship W2560147335A5031937532 @default.
• W2560147335 hasAuthorship W2560147335A5034393745 @default.
• W2560147335 hasAuthorship W2560147335A5036246648 @default.
• W2560147335 hasAuthorship W2560147335A5036753990 @default.
• W2560147335 hasAuthorship W2560147335A5039757859 @default.
• W2560147335 hasAuthorship W2560147335A5042609656 @default.
• W2560147335 hasAuthorship W2560147335A5045814742 @default.
• W2560147335 hasAuthorship W2560147335A5046536404 @default.
• W2560147335 hasAuthorship W2560147335A5057593886 @default.
• W2560147335 hasAuthorship W2560147335A5058459820 @default.
• W2560147335 hasAuthorship W2560147335A5068687462 @default.
• W2560147335 hasAuthorship W2560147335A5069218034 @default.
• W2560147335 hasAuthorship W2560147335A5070129134 @default.
• W2560147335 hasAuthorship W2560147335A5070417705 @default.
• W2560147335 hasAuthorship W2560147335A5072698345 @default.
• W2560147335 hasAuthorship W2560147335A5074331393 @default.
• W2560147335 hasAuthorship W2560147335A5077721366 @default.
• W2560147335 hasAuthorship W2560147335A5078192708 @default.
• W2560147335 hasConcept C101762097 @default.
• W2560147335 hasConcept C104317684 @default.
• W2560147335 hasConcept C119056186 @default.
• W2560147335 hasConcept C150194340 @default.
• W2560147335 hasConcept C153911025 @default.
• W2560147335 hasConcept C189235521 @default.
• W2560147335 hasConcept C190727270 @default.
• W2560147335 hasConcept C2778264664 @default.
• W2560147335 hasConcept C33288867 @default.
• W2560147335 hasConcept C41091548 @default.
• W2560147335 hasConcept C502942594 @default.
• W2560147335 hasConcept C54355233 @default.
• W2560147335 hasConcept C555283112 @default.
• W2560147335 hasConcept C86803240 @default.
• W2560147335 hasConceptScore W2560147335C101762097 @default.
• W2560147335 hasConceptScore W2560147335C104317684 @default.
• W2560147335 hasConceptScore W2560147335C119056186 @default.
• W2560147335 hasConceptScore W2560147335C150194340 @default.
• W2560147335 hasConceptScore W2560147335C153911025 @default.
• W2560147335 hasConceptScore W2560147335C189235521 @default.
• W2560147335 hasConceptScore W2560147335C190727270 @default.
• W2560147335 hasConceptScore W2560147335C2778264664 @default.
• W2560147335 hasConceptScore W2560147335C33288867 @default.
• W2560147335 hasConceptScore W2560147335C41091548 @default.
• W2560147335 hasConceptScore W2560147335C502942594 @default.
• W2560147335 hasConceptScore W2560147335C54355233 @default.
• W2560147335 hasConceptScore W2560147335C555283112 @default.
• W2560147335 hasConceptScore W2560147335C86803240 @default.
• W2560147335 hasIssue "11" @default.
• W2560147335 hasLocation W25601473351 @default.
• W2560147335 hasOpenAccess W2560147335 @default.
• W2560147335 hasPrimaryLocation W25601473351 @default.

• next