Matches in SemOpenAlex for { <https://semopenalex.org/work/W2560232829> ?p ?o ?g. }
- W2560232829 abstract "Abstract In this work we have investigated the genetic changes underlying the high glutathione (GSH) production showed by the evolved Saccharomyces cerevisiae strain UMCC 2581, selected in a molybdate-enriched environment after sexual recombination of the parental wine strain UMCC 855. To reach our goal, we first generated strains with the desired phenotype, and then we mapped changes underlying adaptation to molybdate by using a whole-genome sequencing. Moreover, we carried out the RNA-seq that allowed an accurate measurement of gene expression and an effective comparison between the transcriptional profiles of parental and evolved strains, in order to investigate the relationship between genotype and high GSH production phenotype. Among all genes evaluated only two genes, MED2 and RIM15 both related to oxidative stress response, presented new mutations in the UMCC 2581 strain sequence and were potentially related to the evolved phenotype. Regarding the expression of high GSH production phenotype, it included over-expression of amino acids permeases and precursor biosynthetic enzymes rather than the two GSH metabolic enzymes, whereas GSH production and metabolism, transporter activity, vacuolar detoxification and oxidative stress response enzymes were probably added resulting in the molybdate resistance phenotype. This work provides an example of a combination of an evolution-based strategy to successful obtain yeast strain with desired phenotype and inverse engineering approach to genetic characterize the evolved strain. The obtained genetic information could be useful for further optimization of the evolved strains and for providing an even more rapid approach to identify new strains, with a high GSH production, through a marked-assisted selection strategy." @default.
- W2560232829 created "2016-12-16" @default.
- W2560232829 creator A5014443770 @default.
- W2560232829 creator A5026044975 @default.
- W2560232829 creator A5036306376 @default.
- W2560232829 creator A5046232429 @default.
- W2560232829 date "2016-12-06" @default.
- W2560232829 modified "2023-10-17" @default.
- W2560232829 title "Understanding genetic changes underlying the molybdate resistance and the glutathione production inSaccharomyces cerevisiaewine strains using an evolution-based strategy" @default.
- W2560232829 cites W1485747204 @default.
- W2560232829 cites W152801443 @default.
- W2560232829 cites W1914399831 @default.
- W2560232829 cites W1966380352 @default.
- W2560232829 cites W1971241450 @default.
- W2560232829 cites W1976348985 @default.
- W2560232829 cites W1981392199 @default.
- W2560232829 cites W1982590520 @default.
- W2560232829 cites W1984708728 @default.
- W2560232829 cites W1985341534 @default.
- W2560232829 cites W1987196385 @default.
- W2560232829 cites W1995716972 @default.
- W2560232829 cites W1999514736 @default.
- W2560232829 cites W2001439730 @default.
- W2560232829 cites W2002156802 @default.
- W2560232829 cites W2002622198 @default.
- W2560232829 cites W2007395913 @default.
- W2560232829 cites W2008431238 @default.
- W2560232829 cites W2010579586 @default.
- W2560232829 cites W2017811519 @default.
- W2560232829 cites W2018755699 @default.
- W2560232829 cites W2019271341 @default.
- W2560232829 cites W2024969564 @default.
- W2560232829 cites W2030071974 @default.
- W2560232829 cites W2030728458 @default.
- W2560232829 cites W2037199255 @default.
- W2560232829 cites W2038949753 @default.
- W2560232829 cites W2041296766 @default.
- W2560232829 cites W2043729029 @default.
- W2560232829 cites W2052804740 @default.
- W2560232829 cites W2058737346 @default.
- W2560232829 cites W2059641675 @default.
- W2560232829 cites W2061601404 @default.
- W2560232829 cites W2063012351 @default.
- W2560232829 cites W2063347034 @default.
- W2560232829 cites W2076388225 @default.
- W2560232829 cites W2082121926 @default.
- W2560232829 cites W2083854144 @default.
- W2560232829 cites W2089393581 @default.
- W2560232829 cites W2090041076 @default.
- W2560232829 cites W2090716252 @default.
- W2560232829 cites W2095516190 @default.
- W2560232829 cites W2099088869 @default.
- W2560232829 cites W2102221598 @default.
- W2560232829 cites W2103441770 @default.
- W2560232829 cites W2106578986 @default.
- W2560232829 cites W2115470699 @default.
- W2560232829 cites W2116734717 @default.
- W2560232829 cites W2117131162 @default.
- W2560232829 cites W2121331980 @default.
- W2560232829 cites W2124442087 @default.
- W2560232829 cites W2124731902 @default.
- W2560232829 cites W2132937492 @default.
- W2560232829 cites W2135557896 @default.
- W2560232829 cites W2135992596 @default.
- W2560232829 cites W2136182972 @default.
- W2560232829 cites W2138447779 @default.
- W2560232829 cites W2141906729 @default.
- W2560232829 cites W2145942144 @default.
- W2560232829 cites W2149342156 @default.
- W2560232829 cites W2149399235 @default.
- W2560232829 cites W2150091336 @default.
- W2560232829 cites W2152239989 @default.
- W2560232829 cites W2159993363 @default.
- W2560232829 cites W2161000162 @default.
- W2560232829 cites W2161450172 @default.
- W2560232829 cites W2165238717 @default.
- W2560232829 cites W2167450976 @default.
- W2560232829 cites W2168133698 @default.
- W2560232829 cites W2169357831 @default.
- W2560232829 cites W2170551349 @default.
- W2560232829 cites W2171030481 @default.
- W2560232829 cites W2172973308 @default.
- W2560232829 cites W2175732162 @default.
- W2560232829 cites W277278907 @default.
- W2560232829 cites W4246932843 @default.
- W2560232829 doi "https://doi.org/10.1101/092007" @default.
- W2560232829 hasPublicationYear "2016" @default.
- W2560232829 type Work @default.
- W2560232829 sameAs 2560232829 @default.
- W2560232829 citedByCount "0" @default.
- W2560232829 crossrefType "posted-content" @default.
- W2560232829 hasAuthorship W2560232829A5014443770 @default.
- W2560232829 hasAuthorship W2560232829A5026044975 @default.
- W2560232829 hasAuthorship W2560232829A5036306376 @default.
- W2560232829 hasAuthorship W2560232829A5046232429 @default.
- W2560232829 hasBestOaLocation W25602328291 @default.
- W2560232829 hasConcept C104317684 @default.
- W2560232829 hasConcept C105702510 @default.
- W2560232829 hasConcept C127716648 @default.
- W2560232829 hasConcept C181199279 @default.