FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2560353721> ?p ?o ?g. }

• W2560353721 abstract "Response surface methodology was used to enhance the production of α-galactosidase from Fusarium moniliforme NCIM 1099 in solid-state fermentation. Plackett–Burman design was employed for selection of critical media constituents which were optimized by central composite rotatable design. Wheat bran, peptone and FeSO4·7H2O were identified as significant medium components using PB design. Further CCRD optimized medium components as wheat bran; 4.62 μg, peptone; 315.42 μg, FeSO4·7H2O; 9.04 μg. RSM methodological optimization increased the enzyme production from 13.17 to 207.33 U/g showing 15.74-fold enhancement. The α-galactosidase was purified by 70% fractionation followed by DEAE anion exchange column chromatography which yields 23.33% with 28.68-fold purification. The molecular weight of α-galactosidase was 57 kDa which was determined by SDS-PAGE analysis. Purified enzyme has optimum pH of 4.0 and was found to be stable in wide pH range of 3.0–9.0. Its optimum temperature was 50 °C, whereas its activity remains above 50% up to 2 h at 75 °C. Hg2+ was found to be a potent inhibitor and Mg2+ acted as an activator of enzyme. No significant change was observed in enzyme activity for galactose concentration, ranging from 1 to 100 mM. The K m values of enzyme for substrates p-nitrophenyl-α-d-galactopyranoside, melibiose and raffinose were 0.20, 1.36, and 3.66 mM, respectively. Low K m and stability to various physiological conditions of enzyme represents its potential which can be exploited in various industrial applications." @default.
• W2560353721 created "2016-12-16" @default.
• W2560353721 creator A5023420633 @default.
• W2560353721 creator A5028512481 @default.
• W2560353721 creator A5029417128 @default.
• W2560353721 date "2016-12-01" @default.
• W2560353721 modified "2023-10-03" @default.
• W2560353721 title "Response surface methodology-based optimization of production media and purification of α-galactosidase in solid-state fermentation by Fusarium moniliforme NCIM 1099" @default.
• W2560353721 cites W104412803 @default.
• W2560353721 cites W1562315308 @default.
• W2560353721 cites W1607186791 @default.
• W2560353721 cites W1775749144 @default.
• W2560353721 cites W1963810884 @default.
• W2560353721 cites W1974652716 @default.
• W2560353721 cites W1975568179 @default.
• W2560353721 cites W1980495072 @default.
• W2560353721 cites W1985709215 @default.
• W2560353721 cites W1992634272 @default.
• W2560353721 cites W1997841892 @default.
• W2560353721 cites W1999813071 @default.
• W2560353721 cites W2026922892 @default.
• W2560353721 cites W2028562605 @default.
• W2560353721 cites W2030924993 @default.
• W2560353721 cites W2040020162 @default.
• W2560353721 cites W2044136765 @default.
• W2560353721 cites W2046037112 @default.
• W2560353721 cites W2053097047 @default.
• W2560353721 cites W2053334855 @default.
• W2560353721 cites W2063986338 @default.
• W2560353721 cites W2067025152 @default.
• W2560353721 cites W2068241730 @default.
• W2560353721 cites W2073230901 @default.
• W2560353721 cites W2088389948 @default.
• W2560353721 cites W2089804446 @default.
• W2560353721 cites W2092245143 @default.
• W2560353721 cites W2094410487 @default.
• W2560353721 cites W2094505501 @default.
• W2560353721 cites W2097164174 @default.
• W2560353721 cites W2121996697 @default.
• W2560353721 cites W2122782494 @default.
• W2560353721 cites W2157913345 @default.
• W2560353721 cites W2167692230 @default.
• W2560353721 cites W2167903265 @default.
• W2560353721 cites W2172012474 @default.
• W2560353721 cites W2339598227 @default.
• W2560353721 cites W2531891832 @default.
• W2560353721 cites W2987728674 @default.
• W2560353721 cites W4237342820 @default.
• W2560353721 cites W593292742 @default.
• W2560353721 cites W1585315258 @default.
• W2560353721 doi "https://doi.org/10.1007/s13205-016-0575-7" @default.
• W2560353721 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5148754" @default.
• W2560353721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28330332" @default.
• W2560353721 hasPublicationYear "2016" @default.
• W2560353721 type Work @default.
• W2560353721 sameAs 2560353721 @default.
• W2560353721 citedByCount "14" @default.
• W2560353721 countsByYear W25603537212017 @default.
• W2560353721 countsByYear W25603537212018 @default.
• W2560353721 countsByYear W25603537212019 @default.
• W2560353721 countsByYear W25603537212020 @default.
• W2560353721 countsByYear W25603537212021 @default.
• W2560353721 countsByYear W25603537212022 @default.
• W2560353721 countsByYear W25603537212023 @default.
• W2560353721 crossrefType "journal-article" @default.
• W2560353721 hasAuthorship W2560353721A5023420633 @default.
• W2560353721 hasAuthorship W2560353721A5028512481 @default.
• W2560353721 hasAuthorship W2560353721A5029417128 @default.
• W2560353721 hasBestOaLocation W25603537211 @default.
• W2560353721 hasConcept C100544194 @default.
• W2560353721 hasConcept C111374699 @default.
• W2560353721 hasConcept C150077022 @default.
• W2560353721 hasConcept C178790620 @default.
• W2560353721 hasConcept C181199279 @default.
• W2560353721 hasConcept C185592680 @default.
• W2560353721 hasConcept C206139338 @default.
• W2560353721 hasConcept C2776873940 @default.
• W2560353721 hasConcept C2778139943 @default.
• W2560353721 hasConcept C2778597767 @default.
• W2560353721 hasConcept C2779942233 @default.
• W2560353721 hasConcept C2779998883 @default.
• W2560353721 hasConcept C28212737 @default.
• W2560353721 hasConcept C31903555 @default.
• W2560353721 hasConcept C43617362 @default.
• W2560353721 hasConcept C55493867 @default.
• W2560353721 hasConcept C92397422 @default.
• W2560353721 hasConcept C97428945 @default.
• W2560353721 hasConceptScore W2560353721C100544194 @default.
• W2560353721 hasConceptScore W2560353721C111374699 @default.
• W2560353721 hasConceptScore W2560353721C150077022 @default.
• W2560353721 hasConceptScore W2560353721C178790620 @default.
• W2560353721 hasConceptScore W2560353721C181199279 @default.
• W2560353721 hasConceptScore W2560353721C185592680 @default.
• W2560353721 hasConceptScore W2560353721C206139338 @default.
• W2560353721 hasConceptScore W2560353721C2776873940 @default.
• W2560353721 hasConceptScore W2560353721C2778139943 @default.
• W2560353721 hasConceptScore W2560353721C2778597767 @default.
• W2560353721 hasConceptScore W2560353721C2779942233 @default.
• W2560353721 hasConceptScore W2560353721C2779998883 @default.
• W2560353721 hasConceptScore W2560353721C28212737 @default.

• next