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• W2560360838 abstract "A simple and efficient transformation method is important for plant genetic breeding and gene function analysis. In this study, we developed a in planta transformation approach for pommelo (Citrus maxima), which includes seed germination, seedling decapitation, Parafilm funnel-type-winding, Agrobacterium infection, co-culture, selective-culture, dark treatment for buds induction, transgene integration confirmation and transgenic plant propagation. The highest transformation efficiency (20.41%) was obtained when using hygromycin as selection marker, followed by Basta (19.37%) and Kanamycin (3.21%). Transgenic plants obtained using this method could be graft-propagated in 3 months post transformation, which is shorter than the tissue-culture-dependent transformation. The transformation protocol presented here has great potential to be used for citrus genetic breeding and improvement." @default.
• W2560360838 created "2016-12-16" @default.
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• W2560360838 date "2017-01-01" @default.
• W2560360838 modified "2023-09-27" @default.
• W2560360838 title "A simple and efficient in planta transformation method for pommelo ( Citrus maxima ) using Agrobacterium tumefaciens" @default.
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• W2560360838 doi "https://doi.org/10.1016/j.scienta.2016.11.033" @default.
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