FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2560403812> ?p ?o ?g. }

• W2560403812 abstract "Background MicroRNA miR‐214 has been implicated in many biological cellular functions, but the impact of miR‐214 and its target genes on vascular smooth muscle cell ( VSMC ) proliferation, migration, and neointima smooth muscle cell hyperplasia is unknown. Methods and Results Expression of miR‐214 was closely regulated by different pathogenic stimuli in VSMC s through a transcriptional mechanism and decreased in response to vascular injury. Overexpression of miR‐214 in serum‐starved VSMC s significantly decreased VSMC proliferation and migration, whereas knockdown of miR‐214 dramatically increased VSMC proliferation and migration. Gene and protein biochemical assays, including proteomic analyses, showed that NCK associated protein 1 ( NCKAP 1)—a major component of the WAVE complex that regulates lamellipodia formation and cell motility—was negatively regulated by miR‐214 in VSMC s. Luciferase assays showed that miR‐214 substantially repressed wild‐type but not the miR‐214 binding site mutated version of NCKAP 1 3′ untranslated region luciferase activity in VSMC s. This result confirmed that NCKAP 1 is the functional target of miR‐214 in VSMC s. NCKAP 1 knockdown in VSMC s recapitulates the inhibitory effects of miR‐214 overexpression on actin polymerization, cell migration, and proliferation. Data from cotransfection experiments also revealed that inhibition of NCKAP 1 is required for miR‐214–mediated lamellipodia formation, cell motility, and growth. Importantly, locally enforced expression of miR‐214 in the injured vessels significantly reduced NCKAP 1 expression levels, inhibited VSMC proliferation, and prevented neointima smooth muscle cell hyperplasia after injury. Conclusions We uncovered an important role of miR‐214 and its target gene NCKAP 1 in modulating VSMC functions and neointima hyperplasia. Our findings suggest that miR‐214 represents a potential therapeutic target for vascular diseases." @default.
• W2560403812 created "2016-12-16" @default.
• W2560403812 creator A5044706706 @default.
• W2560403812 creator A5046510449 @default.
• W2560403812 creator A5051241309 @default.
• W2560403812 creator A5061708528 @default.
• W2560403812 creator A5064172105 @default.
• W2560403812 creator A5066716873 @default.
• W2560403812 creator A5068399979 @default.
• W2560403812 creator A5075596275 @default.
• W2560403812 creator A5078465675 @default.
• W2560403812 creator A5083969311 @default.
• W2560403812 creator A5084192970 @default.
• W2560403812 creator A5091189527 @default.
• W2560403812 date "2016-12-19" @default.
• W2560403812 modified "2023-10-12" @default.
• W2560403812 title "NCK Associated Protein 1 Modulated by miRNA‐214 Determines Vascular Smooth Muscle Cell Migration, Proliferation, and Neointima Hyperplasia" @default.
• W2560403812 cites W1169139429 @default.
• W2560403812 cites W1540376590 @default.
• W2560403812 cites W1563575450 @default.
• W2560403812 cites W1649848679 @default.
• W2560403812 cites W1799016756 @default.
• W2560403812 cites W1965867974 @default.
• W2560403812 cites W1978141303 @default.
• W2560403812 cites W1999565947 @default.
• W2560403812 cites W2001165945 @default.
• W2560403812 cites W2005406090 @default.
• W2560403812 cites W2005889517 @default.
• W2560403812 cites W2010106835 @default.
• W2560403812 cites W2010157566 @default.
• W2560403812 cites W2014946489 @default.
• W2560403812 cites W2018621592 @default.
• W2560403812 cites W2022629445 @default.
• W2560403812 cites W2030730244 @default.
• W2560403812 cites W2034890935 @default.
• W2560403812 cites W2036409505 @default.
• W2560403812 cites W2042113238 @default.
• W2560403812 cites W2042811126 @default.
• W2560403812 cites W2051058639 @default.
• W2560403812 cites W2051403674 @default.
• W2560403812 cites W2059493282 @default.
• W2560403812 cites W2063527102 @default.
• W2560403812 cites W2068784971 @default.
• W2560403812 cites W2069841672 @default.
• W2560403812 cites W2070640670 @default.
• W2560403812 cites W2070660616 @default.
• W2560403812 cites W2075317528 @default.
• W2560403812 cites W2076165364 @default.
• W2560403812 cites W2088931568 @default.
• W2560403812 cites W2092842296 @default.
• W2560403812 cites W2094320086 @default.
• W2560403812 cites W2094940134 @default.
• W2560403812 cites W2096166765 @default.
• W2560403812 cites W2097767118 @default.
• W2560403812 cites W2098302826 @default.
• W2560403812 cites W2100592123 @default.
• W2560403812 cites W2101579992 @default.
• W2560403812 cites W2102569350 @default.
• W2560403812 cites W2104619025 @default.
• W2560403812 cites W2105809801 @default.
• W2560403812 cites W2106391242 @default.
• W2560403812 cites W2108182793 @default.
• W2560403812 cites W2108661174 @default.
• W2560403812 cites W2110315980 @default.
• W2560403812 cites W2114685559 @default.
• W2560403812 cites W2120156784 @default.
• W2560403812 cites W2121025268 @default.
• W2560403812 cites W2122826545 @default.
• W2560403812 cites W2130489491 @default.
• W2560403812 cites W2136220732 @default.
• W2560403812 cites W2138344908 @default.
• W2560403812 cites W2140723469 @default.
• W2560403812 cites W2145091080 @default.
• W2560403812 cites W2145109145 @default.
• W2560403812 cites W2150344589 @default.
• W2560403812 cites W2153343747 @default.
• W2560403812 cites W2154993767 @default.
• W2560403812 cites W2155290141 @default.
• W2560403812 cites W2155737281 @default.
• W2560403812 cites W2157483701 @default.
• W2560403812 cites W2161693225 @default.
• W2560403812 cites W2171241454 @default.
• W2560403812 cites W2276317604 @default.
• W2560403812 cites W2417089533 @default.
• W2560403812 cites W4231032814 @default.
• W2560403812 doi "https://doi.org/10.1161/jaha.116.004629" @default.
• W2560403812 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5210428" @default.
• W2560403812 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27927633" @default.
• W2560403812 hasPublicationYear "2016" @default.
• W2560403812 type Work @default.
• W2560403812 sameAs 2560403812 @default.
• W2560403812 citedByCount "40" @default.
• W2560403812 countsByYear W25604038122017 @default.
• W2560403812 countsByYear W25604038122018 @default.
• W2560403812 countsByYear W25604038122019 @default.
• W2560403812 countsByYear W25604038122020 @default.
• W2560403812 countsByYear W25604038122021 @default.
• W2560403812 countsByYear W25604038122022 @default.
• W2560403812 countsByYear W25604038122023 @default.
• W2560403812 crossrefType "journal-article" @default.

• next