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• W2560466848 abstract "In plants triosephosphate isomerase (TPI) interconverts glyceraldehyde 3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP) during glycolysis, gluconeogenesis, and the Calvin-Benson cycle. The nuclear genome of land plants encodes two tpi genes, one gene product is located in the cytoplasm and the other is imported into the chloroplast. Herein we report the crystal structures of the TPIs from the vascular plant Arabidopsis thaliana (AtTPIs) and address their enzymatic modulation by redox agents. Cytoplasmic TPI (cTPI) and chloroplast TPI (pdTPI) share more than 60% amino acid identity and assemble as (β-α)8 dimers with high structural homology. cTPI and pdTPI harbor two and one accessible thiol groups per monomer respectively. cTPI and pdTPI present a cysteine at an equivalent structural position (C13 and C15 respectively) and cTPI also contains a specific solvent accessible cysteine at residue 218 (cTPI-C218). Site directed mutagenesis of residues pdTPI-C15, cTPI-C13 and cTPI-C218 to serine substantially decreases enzymatic activity, indicating that the structural integrity of these cysteines is necessary for catalysis. AtTPIs exhibit differential responses to oxidative agents, cTPI is susceptible to oxidative agents such as diamide and H2O2, whereas pdTPI is resistant to inhibition. Incubation of AtTPIs with the sulfhydryl conjugating reagents methylmethane thiosulfonate (MMTS) and glutathione inhibits enzymatic activity. However, the concentration necessary to inhibit pdTPI is at least two orders of magnitude higher than the concentration needed to inhibit cTPI. Western-blot analysis indicates that residues cTPI-C13, cTPI-C218, and pdTPI-C15 conjugate with glutathione. In summary, our data indicate that AtTPIs could be redox regulated by the derivatization of specific AtTPI cysteines (cTPI-C13 and pdTPI-C15 and cTPI-C218). Since AtTPIs have evolved by gene duplication, the higher resistance of pdTPI to redox agents may be an adaptive consequence to the redox environment in the chloroplast." @default.
• W2560466848 created "2016-12-16" @default.
• W2560466848 creator A5002433394 @default.
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• W2560466848 creator A5016300314 @default.
• W2560466848 creator A5058451525 @default.
• W2560466848 creator A5064129435 @default.
• W2560466848 creator A5074424306 @default.
• W2560466848 date "2016-12-06" @default.
• W2560466848 modified "2023-10-18" @default.
• W2560466848 title "Structural Basis for Redox Regulation of Cytoplasmic and Chloroplastic Triosephosphate Isomerases from Arabidopsis thaliana" @default.
• W2560466848 cites W1482426105 @default.
• W2560466848 cites W1494075612 @default.
• W2560466848 cites W1563653502 @default.
• W2560466848 cites W1663550851 @default.
• W2560466848 cites W1857102957 @default.
• W2560466848 cites W1964325688 @default.
• W2560466848 cites W1966632369 @default.
• W2560466848 cites W1966713407 @default.
• W2560466848 cites W1970782147 @default.
• W2560466848 cites W1974873606 @default.
• W2560466848 cites W1977154773 @default.
• W2560466848 cites W1982378092 @default.
• W2560466848 cites W1982728624 @default.
• W2560466848 cites W1984093270 @default.
• W2560466848 cites W1985535567 @default.
• W2560466848 cites W1986320836 @default.
• W2560466848 cites W1986320888 @default.
• W2560466848 cites W1988521044 @default.
• W2560466848 cites W1990529601 @default.
• W2560466848 cites W1994262781 @default.
• W2560466848 cites W1995766329 @default.
• W2560466848 cites W2006370393 @default.
• W2560466848 cites W2010736306 @default.
• W2560466848 cites W2012447291 @default.
• W2560466848 cites W2012801677 @default.
• W2560466848 cites W2013509150 @default.
• W2560466848 cites W2014162883 @default.
• W2560466848 cites W2021780420 @default.
• W2560466848 cites W2022972602 @default.
• W2560466848 cites W2023882547 @default.
• W2560466848 cites W2027516924 @default.
• W2560466848 cites W2027974246 @default.
• W2560466848 cites W2029328834 @default.
• W2560466848 cites W2030022891 @default.
• W2560466848 cites W2030061336 @default.
• W2560466848 cites W2033629854 @default.
• W2560466848 cites W2033839060 @default.
• W2560466848 cites W2035467972 @default.
• W2560466848 cites W2038258194 @default.
• W2560466848 cites W2041162400 @default.
• W2560466848 cites W2043151807 @default.
• W2560466848 cites W2043925446 @default.
• W2560466848 cites W2048087151 @default.
• W2560466848 cites W2052388444 @default.
• W2560466848 cites W2055438521 @default.
• W2560466848 cites W2065744015 @default.
• W2560466848 cites W2070648292 @default.
• W2560466848 cites W2072355330 @default.
• W2560466848 cites W2072972888 @default.
• W2560466848 cites W2074542695 @default.
• W2560466848 cites W2075215946 @default.
• W2560466848 cites W2077765366 @default.
• W2560466848 cites W2078666864 @default.
• W2560466848 cites W2078927899 @default.
• W2560466848 cites W2082423601 @default.
• W2560466848 cites W2083519557 @default.
• W2560466848 cites W2084804664 @default.
• W2560466848 cites W2087784840 @default.
• W2560466848 cites W2089169279 @default.
• W2560466848 cites W2094725991 @default.
• W2560466848 cites W2095237995 @default.
• W2560466848 cites W2102960554 @default.
• W2560466848 cites W2104752258 @default.
• W2560466848 cites W2107728630 @default.
• W2560466848 cites W2118109148 @default.
• W2560466848 cites W2118768703 @default.
• W2560466848 cites W2120029008 @default.
• W2560466848 cites W2123990511 @default.
• W2560466848 cites W2124659027 @default.
• W2560466848 cites W2132262459 @default.
• W2560466848 cites W2136789143 @default.
• W2560466848 cites W2142070805 @default.
• W2560466848 cites W2143093283 @default.
• W2560466848 cites W2144369707 @default.
• W2560466848 cites W2147778463 @default.
• W2560466848 cites W2147949826 @default.
• W2560466848 cites W2149495661 @default.
• W2560466848 cites W2152537309 @default.
• W2560466848 cites W2153767854 @default.
• W2560466848 cites W2155036894 @default.
• W2560466848 cites W2155451286 @default.
• W2560466848 cites W2156865593 @default.
• W2560466848 cites W2158729487 @default.
• W2560466848 cites W2160219722 @default.
• W2560466848 cites W2161804198 @default.
• W2560466848 cites W2163341755 @default.
• W2560466848 cites W2165327829 @default.

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