Matches in SemOpenAlex for { <https://semopenalex.org/work/W2560591495> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2560591495 endingPage "2698" @default.
- W2560591495 startingPage "2689" @default.
- W2560591495 abstract "The use of manual microscopy for the scoring of chromosome damage in the in vitro micronucleus assay is often associated with user subjectivity. This level of subjectivity can be reduced by using automated platforms, which have added value of faster with high-throughput and multi-endpoint capabilities. However, there is a need to assess the reproducibility and sensitivity of these automated platforms compared with the gold standard of the manual scoring. The automated flow cytometry-based MicroFlow® and image analysis-based Metafer™ were used for dose response analyses in human lymphoblastoid TK6 cells exposed to the model clastogen, methyl methanesulfonate (MMS), aneugen, carbendazim, and the weak genotoxic carcinogen, ochratoxin A (OTA). Cells were treated for 4 or 30 h, with a 26- or 0-h recovery. Flow cytometry scoring parameters and the Metafer™ image classifier were investigated, to assess any potential differences in the micronucleus (MN) dose responses. Dose response data were assessed using the benchmark dose approach with chemical and scoring system set as covariate to assess reproducibility between endpoints. A clear increase in MN frequency was observed using the MicroFlow® approach on TK6 cells treated for 30 h with MMS, carbendazim and OTA. The MicroFlow®-based MN frequencies were comparable to those derived by using the Metafer™ and manual scoring platforms. However, there was a potential overscoring of MN with the MicroFlow® due to the cell lysis step and an underscoring with the Metafer™ system based on current image classifier settings. The findings clearly demonstrate that the MicroFlow® and Metafer™ MN scoring platforms are powerful tools for automated high-throughput MN scoring and dose response analysis." @default.
- W2560591495 created "2016-12-16" @default.
- W2560591495 creator A5007030277 @default.
- W2560591495 creator A5019139090 @default.
- W2560591495 creator A5020495575 @default.
- W2560591495 creator A5031046742 @default.
- W2560591495 creator A5042515109 @default.
- W2560591495 creator A5043298139 @default.
- W2560591495 creator A5057332050 @default.
- W2560591495 date "2016-12-10" @default.
- W2560591495 modified "2023-09-27" @default.
- W2560591495 title "Evaluation of the automated MicroFlow® and Metafer™ platforms for high-throughput micronucleus scoring and dose response analysis in human lymphoblastoid TK6 cells" @default.
- W2560591495 cites W1708405909 @default.
- W2560591495 cites W1919456477 @default.
- W2560591495 cites W1971913419 @default.
- W2560591495 cites W1981602682 @default.
- W2560591495 cites W1990711550 @default.
- W2560591495 cites W2010737160 @default.
- W2560591495 cites W2023200988 @default.
- W2560591495 cites W2024264346 @default.
- W2560591495 cites W2050248204 @default.
- W2560591495 cites W2062642912 @default.
- W2560591495 cites W2066357142 @default.
- W2560591495 cites W2092846542 @default.
- W2560591495 cites W2094161519 @default.
- W2560591495 cites W2129167503 @default.
- W2560591495 cites W2130937946 @default.
- W2560591495 cites W2149620693 @default.
- W2560591495 cites W2159328324 @default.
- W2560591495 cites W2160952168 @default.
- W2560591495 cites W2182405473 @default.
- W2560591495 cites W2243171106 @default.
- W2560591495 cites W2302607968 @default.
- W2560591495 cites W4255738197 @default.
- W2560591495 doi "https://doi.org/10.1007/s00204-016-1903-8" @default.
- W2560591495 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5489585" @default.
- W2560591495 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27942789" @default.
- W2560591495 hasPublicationYear "2016" @default.
- W2560591495 type Work @default.
- W2560591495 sameAs 2560591495 @default.
- W2560591495 citedByCount "16" @default.
- W2560591495 countsByYear W25605914952018 @default.
- W2560591495 countsByYear W25605914952019 @default.
- W2560591495 countsByYear W25605914952020 @default.
- W2560591495 countsByYear W25605914952021 @default.
- W2560591495 countsByYear W25605914952022 @default.
- W2560591495 crossrefType "journal-article" @default.
- W2560591495 hasAuthorship W2560591495A5007030277 @default.
- W2560591495 hasAuthorship W2560591495A5019139090 @default.
- W2560591495 hasAuthorship W2560591495A5020495575 @default.
- W2560591495 hasAuthorship W2560591495A5031046742 @default.
- W2560591495 hasAuthorship W2560591495A5042515109 @default.
- W2560591495 hasAuthorship W2560591495A5043298139 @default.
- W2560591495 hasAuthorship W2560591495A5057332050 @default.
- W2560591495 hasBestOaLocation W25605914951 @default.
- W2560591495 hasConcept C126322002 @default.
- W2560591495 hasConcept C185592680 @default.
- W2560591495 hasConcept C29730261 @default.
- W2560591495 hasConcept C36681516 @default.
- W2560591495 hasConcept C43617362 @default.
- W2560591495 hasConcept C45080847 @default.
- W2560591495 hasConcept C71924100 @default.
- W2560591495 hasConcept C9893847 @default.
- W2560591495 hasConceptScore W2560591495C126322002 @default.
- W2560591495 hasConceptScore W2560591495C185592680 @default.
- W2560591495 hasConceptScore W2560591495C29730261 @default.
- W2560591495 hasConceptScore W2560591495C36681516 @default.
- W2560591495 hasConceptScore W2560591495C43617362 @default.
- W2560591495 hasConceptScore W2560591495C45080847 @default.
- W2560591495 hasConceptScore W2560591495C71924100 @default.
- W2560591495 hasConceptScore W2560591495C9893847 @default.
- W2560591495 hasFunder F4320320333 @default.
- W2560591495 hasIssue "7" @default.
- W2560591495 hasLocation W25605914951 @default.
- W2560591495 hasLocation W25605914952 @default.
- W2560591495 hasLocation W25605914953 @default.
- W2560591495 hasLocation W25605914954 @default.
- W2560591495 hasLocation W25605914955 @default.
- W2560591495 hasOpenAccess W2560591495 @default.
- W2560591495 hasPrimaryLocation W25605914951 @default.
- W2560591495 hasRelatedWork W146476749 @default.
- W2560591495 hasRelatedWork W1983701042 @default.
- W2560591495 hasRelatedWork W1984082042 @default.
- W2560591495 hasRelatedWork W2004064160 @default.
- W2560591495 hasRelatedWork W2030573484 @default.
- W2560591495 hasRelatedWork W2069754044 @default.
- W2560591495 hasRelatedWork W2345575539 @default.
- W2560591495 hasRelatedWork W2357952934 @default.
- W2560591495 hasRelatedWork W4294817530 @default.
- W2560591495 hasRelatedWork W4312238287 @default.
- W2560591495 hasVolume "91" @default.
- W2560591495 isParatext "false" @default.
- W2560591495 isRetracted "false" @default.
- W2560591495 magId "2560591495" @default.
- W2560591495 workType "article" @default.