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- W2560779407 abstract "An ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry method for the simultaneous quantification of chlorpropamide, glibenclamide, gliclazide, glimepiride, metformin, nateglinide, pioglitazone, rosiglitazone, and vildagliptin in human plasma was developed and validated, using isoniazid and sulfaquinoxaline as internal standards. Following plasma protein precipitation using acetonitrile with 1% formic acid, chromatographic separation was performed on a cyano column using gradient elution with water and acetonitrile, both containing 0.1% formic acid. Detection was performed in a quadrupole time-of-flight analyzer, using electrospray ionization operated in the positive mode. Data from validation studies demonstrated that the new method is highly sensitive, selective, precise (RSD < 10%), accurate (RE < 12%), linear (r > 0.99), free of matrix and has no residual effects. The developed method was successfully applied to volunteers' plasma samples. Hence, this method was demonstrated to be appropriate for clinical monitoring of antidiabetic agents." @default.
- W2560779407 created "2016-12-16" @default.
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- W2560779407 date "2016-12-08" @default.
- W2560779407 modified "2023-10-01" @default.
- W2560779407 title "Simultaneous Quantification of Antidiabetic Agents in Human Plasma by a UPLC–QToF-MS Method" @default.
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- W2560779407 doi "https://doi.org/10.1371/journal.pone.0167107" @default.
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