FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2560800613> ?p ?o ?g. }

• W2560800613 abstract "Metabolic stress associated with negative energy balance in high producing dairy cattle and obesity in women is a risk factor for decreased fertility. Non-esterified fatty acids (NEFA) are involved in this pathogenesis as they jeopardize oocyte and embryo development. Growing evidence indicates that maternal metabolic disorders can disturb epigenetic programming, such as DNA methylation, in the offspring. Oocyte maturation and early embryo development coincide with methylation changes and both are sensitive to adverse environments. Therefore, we investigated whether elevated NEFA concentrations affect establishment and maintenance of DNA methylation in oocytes and embryos, subsequently altering transcriptomic profiles and developmental competence of resultant blastocysts.Bovine oocytes and embryos were exposed to different NEFA concentrations in separate experiments. In the first experiment, oocytes were matured in vitro for 24 h in medium containing: 1) physiological (BASAL) concentrations of oleic (OA), palmitic (PA) and stearic (SA) acid or 2) pathophysiological (HIGH COMBI) concentrations of OA, PA and SA. In the second experiment, zygotes were cultivated in vitro for 6.5 days under BASAL or HIGH COMBI conditions. Developmental competence was evaluated by assessing cleavage and blastocyst rate. Overall gene expression and DNA methylation of resultant blastocysts were analyzed using microarray. DNA methylation data were re-evaluated by pyrosequencing. HIGH COMBI-exposed oocytes and embryos displayed a lower competence to develop into blastocysts compared to BASAL-exposed counterparts (19.3% compared to 23.2% and 18.2% compared to 25.3%, respectively) (P < 0.05). HIGH COMBI-exposed oocytes and embryos resulted in blastocysts with altered DNA methylation and transcriptomic fingerprints, compared to BASAL-exposed counterparts. Differences in gene expression and methylation were more pronounced after exposure during culture compared to maturation suggesting that zygotes are more susceptible to adverse environments. Main gene networks affected were related to lipid and carbohydrate metabolism, cell death, immune response and metabolic disorders.Overall, high variation in methylation between blastocysts made it difficult to draw conclusions concerning methylation of individual genes, although a clear overview of affected pathways was obtained. This may offer clues regarding the high rate of embryonic loss and metabolic diseases during later life observed in offspring from mothers displaying lipolytic disorders." @default.
• W2560800613 created "2016-12-16" @default.
• W2560800613 creator A5006390616 @default.
• W2560800613 creator A5016922278 @default.
• W2560800613 creator A5020794426 @default.
• W2560800613 creator A5022912323 @default.
• W2560800613 creator A5025473237 @default.
• W2560800613 creator A5030549611 @default.
• W2560800613 creator A5041986950 @default.
• W2560800613 creator A5075789014 @default.
• W2560800613 creator A5077822614 @default.
• W2560800613 creator A5081669177 @default.
• W2560800613 date "2016-12-01" @default.
• W2560800613 modified "2023-10-16" @default.
• W2560800613 title "Exposure of bovine oocytes and embryos to elevated non-esterified fatty acid concentrations: integration of epigenetic and transcriptomic signatures in resultant blastocysts" @default.
• W2560800613 cites W1503983608 @default.
• W2560800613 cites W1908129098 @default.
• W2560800613 cites W1964480889 @default.
• W2560800613 cites W1964565109 @default.
• W2560800613 cites W1976540956 @default.
• W2560800613 cites W1976686802 @default.
• W2560800613 cites W1978890796 @default.
• W2560800613 cites W1980920372 @default.
• W2560800613 cites W1982615434 @default.
• W2560800613 cites W1984077928 @default.
• W2560800613 cites W1993059448 @default.
• W2560800613 cites W1993243925 @default.
• W2560800613 cites W1994393417 @default.
• W2560800613 cites W1996422090 @default.
• W2560800613 cites W1998117748 @default.
• W2560800613 cites W2000115567 @default.
• W2560800613 cites W2002239849 @default.
• W2560800613 cites W2017228002 @default.
• W2560800613 cites W2031993304 @default.
• W2560800613 cites W2034417921 @default.
• W2560800613 cites W2034515724 @default.
• W2560800613 cites W2036239749 @default.
• W2560800613 cites W2038853397 @default.
• W2560800613 cites W2051712917 @default.
• W2560800613 cites W2052979310 @default.
• W2560800613 cites W2054527561 @default.
• W2560800613 cites W2055679485 @default.
• W2560800613 cites W2061809258 @default.
• W2560800613 cites W2071953998 @default.
• W2560800613 cites W2079973462 @default.
• W2560800613 cites W2081769002 @default.
• W2560800613 cites W2083624932 @default.
• W2560800613 cites W2084380234 @default.
• W2560800613 cites W2091101545 @default.
• W2560800613 cites W2096391240 @default.
• W2560800613 cites W2100338121 @default.
• W2560800613 cites W2102101616 @default.
• W2560800613 cites W2102592982 @default.
• W2560800613 cites W2103707101 @default.
• W2560800613 cites W2104719592 @default.
• W2560800613 cites W2106557257 @default.
• W2560800613 cites W2109730324 @default.
• W2560800613 cites W2116463796 @default.
• W2560800613 cites W2116534551 @default.
• W2560800613 cites W2119783621 @default.
• W2560800613 cites W2121417064 @default.
• W2560800613 cites W2122884201 @default.
• W2560800613 cites W2127847707 @default.
• W2560800613 cites W2128199185 @default.
• W2560800613 cites W2128909276 @default.
• W2560800613 cites W2130355814 @default.
• W2560800613 cites W2131230902 @default.
• W2560800613 cites W2133792826 @default.
• W2560800613 cites W2141446782 @default.
• W2560800613 cites W2142280970 @default.
• W2560800613 cites W2147575721 @default.
• W2560800613 cites W2152189545 @default.
• W2560800613 cites W2153027599 @default.
• W2560800613 cites W2159922117 @default.
• W2560800613 cites W2166337262 @default.
• W2560800613 cites W2170408710 @default.
• W2560800613 cites W843510236 @default.
• W2560800613 doi "https://doi.org/10.1186/s12864-016-3366-y" @default.
• W2560800613 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5146907" @default.
• W2560800613 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27931182" @default.
• W2560800613 hasPublicationYear "2016" @default.
• W2560800613 type Work @default.
• W2560800613 sameAs 2560800613 @default.
• W2560800613 citedByCount "65" @default.
• W2560800613 countsByYear W25608006132017 @default.
• W2560800613 countsByYear W25608006132018 @default.
• W2560800613 countsByYear W25608006132019 @default.
• W2560800613 countsByYear W25608006132020 @default.
• W2560800613 countsByYear W25608006132021 @default.
• W2560800613 countsByYear W25608006132022 @default.
• W2560800613 countsByYear W25608006132023 @default.
• W2560800613 crossrefType "journal-article" @default.
• W2560800613 hasAuthorship W2560800613A5006390616 @default.
• W2560800613 hasAuthorship W2560800613A5016922278 @default.
• W2560800613 hasAuthorship W2560800613A5020794426 @default.
• W2560800613 hasAuthorship W2560800613A5022912323 @default.
• W2560800613 hasAuthorship W2560800613A5025473237 @default.
• W2560800613 hasAuthorship W2560800613A5030549611 @default.
• W2560800613 hasAuthorship W2560800613A5041986950 @default.
• W2560800613 hasAuthorship W2560800613A5075789014 @default.

• next