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- W2561016022 abstract "Since exclusively breast-suckled infants obtain their nutrient only from their mother's milk, it might be anticipated that a correlation will exist between the 15 N/ 14 N isotope ratios of amino acids of protein of young infants and those supplied by their mother. The work presented here aimed to determine whether amino nitrogen transfer from human milk to infant hair protein synthesized within the first month of life conserves the maternal isotopic signature or whether post-ingestion fractionation dominates the nitrogen isotope spectrum. The study was conducted at 1 month post-birth on 100 mother-infant pairs. Isotope ratios 15 N/ 14 N and 13 C/ 12 C were measured using isotope ratio measurement by Mass Spectrometry (irm-MS) for whole maternal milk, and infant hair and 15 N/ 14 N ratios were also measured by GC-irm-MS for the N-pivaloyl-O- isopropyl esters of amino acids obtained from the hydro- lysis of milk and hair proteins. The d 15 N and d 13 C( %) were found to be significantly higher in infant hair than in breast milk (d 15 N, P 0.001; d 13 C, P 0.001). Further- more, the d 15 N( %) of individual amino acids in infant hair was also significantly higher than that in maternal milk (P 0.001). By calculation, the observed shift in isotope ratio was shown not to be accounted for by the amino acid composition of hair and milk proteins, indicating that it is not simply due to differences in the composition in the proteins present. Rather, it would appear that each pool— mother and infant—turns over independently, and that fractionation in infant N-metabolism even in the first month of life dominates over the nutrient N-content." @default.
- W2561016022 created "2017-01-06" @default.
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- W2561016022 date "2013-01-01" @default.
- W2561016022 modified "2023-09-23" @default.
- W2561016022 title "Human baby hair amino acid natural abundance 15 N-isotope values are not related to the 15 N-isotope values of amino acids in mother's breast milk protein" @default.
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