FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2561125625> ?p ?o ?g. }

• W2561125625 endingPage "481" @default.
• W2561125625 startingPage "470" @default.
• W2561125625 abstract "Duchenne muscular dystrophy (DMD) is a genetic disorder in which the absence of dystrophin leads to progressive muscle degeneration and weakness. Although the genetic basis is known, the pathophysiology of dystrophic skeletal muscle remains unclear. We examined nuclear movement in wild-type (WT) and muscular dystrophy mouse model for DMD (MDX) (dystrophin-null) mouse myofibers. We also examined expression of proteins in the linkers of nucleoskeleton and cytoskeleton (LINC) complex, as well as nuclear transcriptional activity via histone H3 acetylation and polyadenylate-binding nuclear protein-1. Because movement of nuclei is not only LINC dependent but also microtubule dependent, we analyzed microtubule density and organization in WT and MDX myofibers, including the application of a unique 3D tool to assess microtubule core structure. Nuclei in MDX myofibers were more mobile than in WT myofibers for both distance traveled and velocity. MDX muscle shows reduced expression and labeling intensity of nesprin-1, a LINC protein that attaches the nucleus to the microtubule and actin cytoskeleton. MDX nuclei also showed altered transcriptional activity. Previous studies established that microtubule structure at the cortex is disrupted in MDX myofibers; our analyses extend these findings by showing that microtubule structure in the core is also disrupted. In addition, we studied malformed MDX myofibers to better understand the role of altered myofiber morphology vs. microtubule architecture in the underlying susceptibility to injury seen in dystrophic muscles. We incorporated morphological and microtubule architectural concepts into a simplified finite element mathematical model of myofiber mechanics, which suggests a greater contribution of myofiber morphology than microtubule structure to muscle biomechanical performance. NEW & NOTEWORTHY Microtubules provide the means for nuclear movement but show altered organization in the muscular dystrophy mouse model (MDX) (dystrophin-null) muscle. Here, MDX myofibers show increased nuclear movement, altered transcriptional activity, and altered linkers of nucleoskeleton and cytoskeleton complex expression compared with healthy myofibers. Microtubule architecture was incorporated in finite element modeling of passive stretch, revealing a role of fiber malformation, commonly found in MDX muscle. The results suggest that alterations in microtubule architecture in MDX muscle affect nuclear movement, which is essential for muscle function." @default.
• W2561125625 created "2017-01-06" @default.
• W2561125625 creator A5018897986 @default.
• W2561125625 creator A5024155695 @default.
• W2561125625 creator A5034656890 @default.
• W2561125625 creator A5062850545 @default.
• W2561125625 creator A5070820264 @default.
• W2561125625 creator A5074655099 @default.
• W2561125625 creator A5083109409 @default.
• W2561125625 creator A5087477510 @default.
• W2561125625 date "2017-03-01" @default.
• W2561125625 modified "2023-10-16" @default.
• W2561125625 title "Altered nuclear dynamics in MDX myofibers" @default.
• W2561125625 cites W1543201338 @default.
• W2561125625 cites W1874007049 @default.
• W2561125625 cites W1916643227 @default.
• W2561125625 cites W1933877372 @default.
• W2561125625 cites W1967886094 @default.
• W2561125625 cites W1969551659 @default.
• W2561125625 cites W1971563489 @default.
• W2561125625 cites W1984699863 @default.
• W2561125625 cites W1987711577 @default.
• W2561125625 cites W1997600667 @default.
• W2561125625 cites W1997767315 @default.
• W2561125625 cites W2001969173 @default.
• W2561125625 cites W2002561225 @default.
• W2561125625 cites W2006525538 @default.
• W2561125625 cites W2010674304 @default.
• W2561125625 cites W2012182366 @default.
• W2561125625 cites W2012794037 @default.
• W2561125625 cites W2016329271 @default.
• W2561125625 cites W2016923561 @default.
• W2561125625 cites W2021540981 @default.
• W2561125625 cites W2026517817 @default.
• W2561125625 cites W2030637965 @default.
• W2561125625 cites W2031857161 @default.
• W2561125625 cites W2034790803 @default.
• W2561125625 cites W2038761244 @default.
• W2561125625 cites W2039460079 @default.
• W2561125625 cites W2046753954 @default.
• W2561125625 cites W2048441156 @default.
• W2561125625 cites W2054025362 @default.
• W2561125625 cites W2055572268 @default.
• W2561125625 cites W2055886634 @default.
• W2561125625 cites W2066180106 @default.
• W2561125625 cites W2068324548 @default.
• W2561125625 cites W2075870854 @default.
• W2561125625 cites W2076918348 @default.
• W2561125625 cites W2081342132 @default.
• W2561125625 cites W2084351192 @default.
• W2561125625 cites W2085778346 @default.
• W2561125625 cites W2096024183 @default.
• W2561125625 cites W2101711109 @default.
• W2561125625 cites W2103409944 @default.
• W2561125625 cites W2108233606 @default.
• W2561125625 cites W2108759409 @default.
• W2561125625 cites W2112114930 @default.
• W2561125625 cites W2113101155 @default.
• W2561125625 cites W2121501753 @default.
• W2561125625 cites W2122182720 @default.
• W2561125625 cites W2128453064 @default.
• W2561125625 cites W2134320165 @default.
• W2561125625 cites W2134764403 @default.
• W2561125625 cites W2137848437 @default.
• W2561125625 cites W2143089027 @default.
• W2561125625 cites W2143370070 @default.
• W2561125625 cites W2150789782 @default.
• W2561125625 cites W2158029007 @default.
• W2561125625 cites W2161895238 @default.
• W2561125625 cites W2162938177 @default.
• W2561125625 cites W2164783275 @default.
• W2561125625 cites W2165168956 @default.
• W2561125625 cites W2165382119 @default.
• W2561125625 cites W2166059867 @default.
• W2561125625 cites W2167416861 @default.
• W2561125625 cites W2171402579 @default.
• W2561125625 cites W2171872743 @default.
• W2561125625 cites W2256535442 @default.
• W2561125625 cites W2273265205 @default.
• W2561125625 cites W2280149859 @default.
• W2561125625 cites W2289177814 @default.
• W2561125625 cites W2299913940 @default.
• W2561125625 cites W2312577323 @default.
• W2561125625 cites W2443440841 @default.
• W2561125625 cites W3100603246 @default.
• W2561125625 cites W4211110504 @default.
• W2561125625 cites W1991215053 @default.
• W2561125625 doi "https://doi.org/10.1152/japplphysiol.00857.2016" @default.
• W2561125625 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5401960" @default.
• W2561125625 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/27979987" @default.
• W2561125625 hasPublicationYear "2017" @default.
• W2561125625 type Work @default.
• W2561125625 sameAs 2561125625 @default.
• W2561125625 citedByCount "24" @default.
• W2561125625 countsByYear W25611256252017 @default.
• W2561125625 countsByYear W25611256252018 @default.
• W2561125625 countsByYear W25611256252019 @default.
• W2561125625 countsByYear W25611256252020 @default.

• next