FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2561279433> ?p ?o ?g. }

• W2561279433 endingPage "10808" @default.
• W2561279433 startingPage "10785" @default.
• W2561279433 abstract "// Latha B. Pathangey 1,* , Dustin B. McCurry 1,* , Sandra J. Gendler 1,2,3 , Ana L. Dominguez 1 , Jessica E. Gorman 1 , Girish Pathangey 1 , Laurie A. Mihalik 3 , Yushe Dang 4 , Mary L. Disis 4 and Peter A. Cohen 2,3 1 Department of Biochemistry and Molecular Biology, Mayo Clinic, Scottsdale, AZ, USA 2 Department of Immunology, Mayo Clinic, Scottsdale, AZ, USA 3 Department of Hematology and Oncology, Mayo Clinic, Scottsdale, AZ, USA 4 Tumor Vaccine Group, Center for Translational Medicine in Women’s Health, University of Washington, Seattle, WA, USA * Shared first authorship Correspondence to: Peter A. Cohen, email: // Sandra J. Gendler, email: // Mary L. Disis, email: // Keywords : PBMC, TLR agonists, GM-CSF, IL-7, adoptive therapy, MUC1 Received : October 07, 2016 Accepted : November 23, 2016 Published : December 11, 2016 Abstract Effective adoptive immunotherapy has proved elusive for many types of human cancer, often due to difficulties achieving robust expansion of natural tumor-specific T-cells from peripheral blood. We hypothesized that antigen-driven T-cell expansion might best be triggered in vitro by acute activation of innate immunity to mimic a life-threatening infection. Unfractionated peripheral blood mononuclear cells (PBMC) were subjected to a two-step culture, first synchronizing their exposure to exogenous antigens with aggressive surrogate activation of innate immunity, followed by γ-chain cytokine-modulated T-cell hyperexpansion. Step 1 exposure to GM-CSF plus paired Toll-like receptor agonists (resiquimod and LPS), stimulated abundant IL-12 and IL-23 secretion, as well as upregulated co-stimulatory molecules and CD11c expression within the myeloid (CD33+) subpopulation. Added synthetic long peptides (>20aa) derived from widely expressed oncoproteins (MUC1, HER2/neu and CMVpp65), were reliably presented to CD4+ T-cells and cross-presented to CD8+ T-cells. Both presentation and cross-presentation demonstrated proteasomal and Sec61 dependence that could bypass the endoplasmic reticulum. Step 2 exposure to exogenous IL-7 or IL-7+IL-2 produced selective and sustained expansion of both CD4+ and CD8+ peptide-specific T-cells with a predominant interferon-γ-producing T1-type, as well as the antigen-specific ability to lyse tumor targets. Other γ-chain cytokines and/or combinations were initially proliferogenic, but followed by a contractile phase not observed with IL-7 or IL-7+IL-2. Regulatory T-cells were minimally propagated under these culture conditions. This mechanistically rational culture sequence, effective even for unvaccinated donors, enables rapid preparation of T-cells recognizing tumor-associated antigens expressed by the majority of human cancers, including pancreatic cancers, breast cancers and glioblastomas." @default.
• W2561279433 created "2017-01-06" @default.
• W2561279433 creator A5013312057 @default.
• W2561279433 creator A5021851431 @default.
• W2561279433 creator A5027281532 @default.
• W2561279433 creator A5027377475 @default.
• W2561279433 creator A5033888720 @default.
• W2561279433 creator A5039848435 @default.
• W2561279433 creator A5053687578 @default.
• W2561279433 creator A5065213314 @default.
• W2561279433 creator A5073417965 @default.
• W2561279433 creator A5082807574 @default.
• W2561279433 date "2016-12-11" @default.
• W2561279433 modified "2023-09-26" @default.
• W2561279433 title "Surrogate <i>in vitro</i> activation of innate immunity synergizes with interleukin-7 to unleash rapid antigen-driven outgrowth of CD4+ and CD8+ human peripheral blood T-cells naturally recognizing MUC1, HER2/neu and other tumor-associated antigens" @default.
• W2561279433 cites W1499854106 @default.
• W2561279433 cites W1503828302 @default.
• W2561279433 cites W1506603838 @default.
• W2561279433 cites W1519770770 @default.
• W2561279433 cites W1575119798 @default.
• W2561279433 cites W1637128412 @default.
• W2561279433 cites W163953079 @default.
• W2561279433 cites W1748564991 @default.
• W2561279433 cites W185336721 @default.
• W2561279433 cites W1898024522 @default.
• W2561279433 cites W1920177081 @default.
• W2561279433 cites W1932934724 @default.
• W2561279433 cites W1945928767 @default.
• W2561279433 cites W1965029629 @default.
• W2561279433 cites W1965359442 @default.
• W2561279433 cites W1971601964 @default.
• W2561279433 cites W1974842026 @default.
• W2561279433 cites W1975456210 @default.
• W2561279433 cites W1981302308 @default.
• W2561279433 cites W1988620205 @default.
• W2561279433 cites W1989773065 @default.
• W2561279433 cites W1992699637 @default.
• W2561279433 cites W1995736586 @default.
• W2561279433 cites W2002567532 @default.
• W2561279433 cites W2004772469 @default.
• W2561279433 cites W2008987290 @default.
• W2561279433 cites W2009648532 @default.
• W2561279433 cites W2020604513 @default.
• W2561279433 cites W2021014181 @default.
• W2561279433 cites W2021472146 @default.
• W2561279433 cites W2022626810 @default.
• W2561279433 cites W2023940632 @default.
• W2561279433 cites W2036096294 @default.
• W2561279433 cites W2037918327 @default.
• W2561279433 cites W2044050227 @default.
• W2561279433 cites W2047139806 @default.
• W2561279433 cites W2051932178 @default.
• W2561279433 cites W2052997401 @default.
• W2561279433 cites W2053346961 @default.
• W2561279433 cites W2053654593 @default.
• W2561279433 cites W2063667712 @default.
• W2561279433 cites W2066141983 @default.
• W2561279433 cites W2067551077 @default.
• W2561279433 cites W2068696470 @default.
• W2561279433 cites W2072710870 @default.
• W2561279433 cites W2073107785 @default.
• W2561279433 cites W2074801788 @default.
• W2561279433 cites W2085890670 @default.
• W2561279433 cites W2090536138 @default.
• W2561279433 cites W2094998207 @default.
• W2561279433 cites W2098772563 @default.
• W2561279433 cites W2100083914 @default.
• W2561279433 cites W2103200148 @default.
• W2561279433 cites W2104157195 @default.
• W2561279433 cites W2113051617 @default.
• W2561279433 cites W2115094537 @default.
• W2561279433 cites W2115678488 @default.
• W2561279433 cites W2116407378 @default.
• W2561279433 cites W2119000942 @default.
• W2561279433 cites W2120020619 @default.
• W2561279433 cites W2123506425 @default.
• W2561279433 cites W2127782698 @default.
• W2561279433 cites W2128276505 @default.
• W2561279433 cites W2129481598 @default.
• W2561279433 cites W2132847632 @default.
• W2561279433 cites W2137220693 @default.
• W2561279433 cites W2144859024 @default.
• W2561279433 cites W2148177811 @default.
• W2561279433 cites W2148523692 @default.
• W2561279433 cites W2148785609 @default.
• W2561279433 cites W2150173080 @default.
• W2561279433 cites W2151246207 @default.
• W2561279433 cites W2154740756 @default.
• W2561279433 cites W2160289525 @default.
• W2561279433 cites W2161618738 @default.
• W2561279433 cites W2164842455 @default.
• W2561279433 cites W2165119413 @default.
• W2561279433 cites W2165463990 @default.
• W2561279433 cites W2166630199 @default.
• W2561279433 cites W2167104711 @default.
• W2561279433 cites W2167341854 @default.
• W2561279433 cites W2168935120 @default.
• W2561279433 cites W2169963308 @default.

• next