FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2561290773> ?p ?o ?g. }

• W2561290773 endingPage "187" @default.
• W2561290773 startingPage "187" @default.
• W2561290773 abstract "Abstract Prostate Cancer (PCa) is the second most common cause of cancer-related deaths in American men, mostly related to the development of androgen-independent PCa (AIPC). Understanding of the molecular mechanisms underlying the development of AIPC is critical to discovering more effective therapeutic strategies for AIPC. MicroRNAs (miRNAs) are short non-coding RNAs that play an important role in post-transcriptional regulation and have been implicated in cancer development. A recently annotated miRNA is miR-1205 encoded by the long non-coding PVT1 gene locus which is located at the prostate cancer susceptibility chromosomal region, 8q24. The role of miR-1205 in PCa is, however, unknown. This study aimed to determine whether miR-1205 plays an essential role in PCa progression. A panel of five prostate cell lines with a diversity of PCa characteristics was used. RWPE1, a non-tumorigenic prostate epithelial cell line, WPE1-NA22, an indolent PCa cell line derived from RWPE1 and three aggressive PCa cell lines (PC3, VCaP and MDA PCa 2b) were used. Expression of miR-1205 was determined using real-time quantitative polymerase chain reaction. We evaluated the effect of the loss or gain of miR-1205 by transfecting the oligonucleotide inhibitor or oligonucleotide mimic respectively. Effect of loss or gain of miR-1205 on proliferation and migration was measured using MTT and wound healing assays, respectively. The miSVR computer algorithm was used to ascertain putative targets. Effect of transfection of oligonucleotide mimic on mRNA expression of the putative targets was used to confirm the miR-1205 target. Our study indicates that the expression of miR-1205 is markedly less in PCa cells (WPE1-NA22, MDA PCa 2b, PC3, and VCaP) in comparison to the RWPE1 non-tumorigenic prostate epithelial cell line. The expression of miR-1205 was further significantly less in AIPC cells (PC3) compared to the other PCa cells. Loss of miR-1205 significantly increased cell proliferation in prostate epithelial cells. Exogenous miR-1205 significantly decreased cell proliferation in prostate epithelial cells. Also, loss of miR-1205 promoted migration in PCa cells by nearly 3 folds. The miSVR computer algorithm identified FRY-like (FRYL) as a putative target of miR-1205. Transfecting the oligonucleotide mimic of miR-1205 into androgen-independent PC3 cells leads to significant decrease in the expression of FRYL, suggesting that FRYL is a direct target of miR-1205. Therefore, miR-1205 regulates proliferation and migration of prostate epithelial cells, and loss of miR-1205 promotes a tumorigenic phenotype in PCa. Moreover, miR-1205 targets FRYL in AIPC. Consequently, strategies to increase miR-1205 or target FRYL may have therapeutic potential in AIPC. Citation Format: Victoria Durojaiye, Adeodat Ilboudo, Fayola Levine, Joseph Osborne, Jong Y. Park, Olorunseun O. Ogunwobi. miR-1205/FRYL as a novel regulatory mechanism in androgen-independent prostate cancer. [abstract]. In: Proceedings of the 106th Annual Meeting of the American Association for Cancer Research; 2015 Apr 18-22; Philadelphia, PA. Philadelphia (PA): AACR; Cancer Res 2015;75(15 Suppl):Abstract nr 187. doi:10.1158/1538-7445.AM2015-187" @default.
• W2561290773 created "2017-01-06" @default.
• W2561290773 creator A5033094068 @default.
• W2561290773 creator A5036798940 @default.
• W2561290773 creator A5053687018 @default.
• W2561290773 creator A5069824664 @default.
• W2561290773 creator A5081407941 @default.
• W2561290773 creator A5083534980 @default.
• W2561290773 date "2015-08-01" @default.
• W2561290773 modified "2023-09-23" @default.
• W2561290773 title "Abstract 187: miR-1205/FRYL as a novel regulatory mechanism in androgen-independent prostate cancer" @default.
• W2561290773 doi "https://doi.org/10.1158/1538-7445.am2015-187" @default.
• W2561290773 hasPublicationYear "2015" @default.
• W2561290773 type Work @default.
• W2561290773 sameAs 2561290773 @default.
• W2561290773 citedByCount "4" @default.
• W2561290773 countsByYear W25612907732018 @default.
• W2561290773 countsByYear W25612907732019 @default.
• W2561290773 countsByYear W25612907732020 @default.
• W2561290773 crossrefType "journal-article" @default.
• W2561290773 hasAuthorship W2561290773A5033094068 @default.
• W2561290773 hasAuthorship W2561290773A5036798940 @default.
• W2561290773 hasAuthorship W2561290773A5053687018 @default.
• W2561290773 hasAuthorship W2561290773A5069824664 @default.
• W2561290773 hasAuthorship W2561290773A5081407941 @default.
• W2561290773 hasAuthorship W2561290773A5083534980 @default.
• W2561290773 hasConcept C104317684 @default.
• W2561290773 hasConcept C121608353 @default.
• W2561290773 hasConcept C145059251 @default.
• W2561290773 hasConcept C2776235491 @default.
• W2561290773 hasConcept C2780192828 @default.
• W2561290773 hasConcept C502942594 @default.
• W2561290773 hasConcept C54355233 @default.
• W2561290773 hasConcept C86803240 @default.
• W2561290773 hasConceptScore W2561290773C104317684 @default.
• W2561290773 hasConceptScore W2561290773C121608353 @default.
• W2561290773 hasConceptScore W2561290773C145059251 @default.
• W2561290773 hasConceptScore W2561290773C2776235491 @default.
• W2561290773 hasConceptScore W2561290773C2780192828 @default.
• W2561290773 hasConceptScore W2561290773C502942594 @default.
• W2561290773 hasConceptScore W2561290773C54355233 @default.
• W2561290773 hasConceptScore W2561290773C86803240 @default.
• W2561290773 hasIssue "15_Supplement" @default.
• W2561290773 hasLocation W25612907731 @default.
• W2561290773 hasOpenAccess W2561290773 @default.
• W2561290773 hasPrimaryLocation W25612907731 @default.
• W2561290773 hasRelatedWork W1980245127 @default.
• W2561290773 hasRelatedWork W2045406164 @default.
• W2561290773 hasRelatedWork W2076984982 @default.
• W2561290773 hasRelatedWork W2106003470 @default.
• W2561290773 hasRelatedWork W2115093090 @default.
• W2561290773 hasRelatedWork W2400504790 @default.
• W2561290773 hasRelatedWork W2433835599 @default.
• W2561290773 hasRelatedWork W2984941165 @default.
• W2561290773 hasRelatedWork W3177803149 @default.
• W2561290773 hasRelatedWork W4362495310 @default.
• W2561290773 hasVolume "75" @default.
• W2561290773 isParatext "false" @default.
• W2561290773 isRetracted "false" @default.
• W2561290773 magId "2561290773" @default.
• W2561290773 workType "article" @default.