Matches in SemOpenAlex for { <https://semopenalex.org/work/W2561775774> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2561775774 endingPage "94" @default.
- W2561775774 startingPage "90" @default.
- W2561775774 abstract "Abstract Background: Snake venom is a complex mixture of biologically active substances. Some peptides and proteins from snake have already demonstrated their therapeutically potential. The venom of Naja haje, an Elapidae member, has been analyzed from this point of view. Understanding the fully biochemical role of its enzymes has determined the scientists to find new separation and identification methods. Objective: Our goal was to develop an optimal HPLC analytical method for separation and identification of Naja haje snake venom components, known for its neurotoxic activity. In addition, we wanted to find out if crude snake venom could inhibit the development of both Gram-positive and Gram-negative bacterial cultures. Materials and Method: Analysis of venom was performed on a HPLC system using a C16 column with UV detection at 210 nm. The analysis was done using two mobile phases, containing different concentrations of acetonitrile and trifluoroacetic acid aqueous solution at different pH values. An elution gradient was set at a flow of 0.60 mL/min. Bactericidal activity was quantified by measuring inhibition diameter around an aseptically disk filled with crude venom using Staphylococcus aureus and Escherichia coli. Results: An optimal HPLC analytical method has been developed by changing different parameters such as the pH value of mobile phase A or the elution gradient. The best resolution were obtained at a pH value of 7.4, in gradient varying from 5% to 45% in mobile phase B. Microbiological studies of the venom showed that Gram-positive bacteria growth was inhibited by crude venom, while on Gram-negative bacteria growth no effect was observed. Inhibition zone is dose-dependent and fresh crude venom is with 30% more potent than venom freeze and kept at -55°C. Conclusions: A comprehensive catalog of venom composition may serve as a starting point for studying structurefunction correlations of individual toxins for the development of new research tools and drugs of potential clinical use." @default.
- W2561775774 created "2017-01-06" @default.
- W2561775774 creator A5029734180 @default.
- W2561775774 creator A5044162050 @default.
- W2561775774 creator A5054272689 @default.
- W2561775774 creator A5058705900 @default.
- W2561775774 creator A5069862249 @default.
- W2561775774 creator A5085680364 @default.
- W2561775774 creator A5087407947 @default.
- W2561775774 date "2016-02-09" @default.
- W2561775774 modified "2023-10-18" @default.
- W2561775774 title "New RP-HPLC Method for Separation of Naja haje haje Venom and Studies of its Bactericidal Effect" @default.
- W2561775774 cites W1044427943 @default.
- W2561775774 cites W1440565807 @default.
- W2561775774 cites W1509539843 @default.
- W2561775774 cites W1526234433 @default.
- W2561775774 cites W1963585954 @default.
- W2561775774 cites W1972008682 @default.
- W2561775774 cites W1976821850 @default.
- W2561775774 cites W1983734540 @default.
- W2561775774 cites W1985692848 @default.
- W2561775774 cites W1991060344 @default.
- W2561775774 cites W1993177180 @default.
- W2561775774 cites W1995398299 @default.
- W2561775774 cites W2003557975 @default.
- W2561775774 cites W2008964357 @default.
- W2561775774 cites W2011342199 @default.
- W2561775774 cites W2023113356 @default.
- W2561775774 cites W2026589902 @default.
- W2561775774 cites W2044410050 @default.
- W2561775774 cites W2049729704 @default.
- W2561775774 cites W2054455972 @default.
- W2561775774 cites W2073032360 @default.
- W2561775774 cites W2084738263 @default.
- W2561775774 cites W2096192115 @default.
- W2561775774 cites W2102863198 @default.
- W2561775774 cites W2124034732 @default.
- W2561775774 cites W2127892885 @default.
- W2561775774 cites W2130764107 @default.
- W2561775774 cites W2134106813 @default.
- W2561775774 cites W2138229667 @default.
- W2561775774 cites W2143720197 @default.
- W2561775774 cites W2165309858 @default.
- W2561775774 cites W2168541231 @default.
- W2561775774 cites W2171525424 @default.
- W2561775774 cites W2334008440 @default.
- W2561775774 cites W2129707413 @default.
- W2561775774 cites W2509356915 @default.
- W2561775774 doi "https://doi.org/10.1515/amma-2015-0113" @default.
- W2561775774 hasPublicationYear "2016" @default.
- W2561775774 type Work @default.
- W2561775774 sameAs 2561775774 @default.
- W2561775774 citedByCount "0" @default.
- W2561775774 crossrefType "journal-article" @default.
- W2561775774 hasAuthorship W2561775774A5029734180 @default.
- W2561775774 hasAuthorship W2561775774A5044162050 @default.
- W2561775774 hasAuthorship W2561775774A5054272689 @default.
- W2561775774 hasAuthorship W2561775774A5058705900 @default.
- W2561775774 hasAuthorship W2561775774A5069862249 @default.
- W2561775774 hasAuthorship W2561775774A5085680364 @default.
- W2561775774 hasAuthorship W2561775774A5087407947 @default.
- W2561775774 hasBestOaLocation W25617757741 @default.
- W2561775774 hasConcept C179998833 @default.
- W2561775774 hasConcept C185592680 @default.
- W2561775774 hasConcept C2779448229 @default.
- W2561775774 hasConcept C43617362 @default.
- W2561775774 hasConcept C55493867 @default.
- W2561775774 hasConcept C71924100 @default.
- W2561775774 hasConcept C73826308 @default.
- W2561775774 hasConceptScore W2561775774C179998833 @default.
- W2561775774 hasConceptScore W2561775774C185592680 @default.
- W2561775774 hasConceptScore W2561775774C2779448229 @default.
- W2561775774 hasConceptScore W2561775774C43617362 @default.
- W2561775774 hasConceptScore W2561775774C55493867 @default.
- W2561775774 hasConceptScore W2561775774C71924100 @default.
- W2561775774 hasConceptScore W2561775774C73826308 @default.
- W2561775774 hasIssue "1" @default.
- W2561775774 hasLocation W25617757741 @default.
- W2561775774 hasOpenAccess W2561775774 @default.
- W2561775774 hasPrimaryLocation W25617757741 @default.
- W2561775774 hasRelatedWork W2018207808 @default.
- W2561775774 hasRelatedWork W2037171905 @default.
- W2561775774 hasRelatedWork W2043512602 @default.
- W2561775774 hasRelatedWork W2043863063 @default.
- W2561775774 hasRelatedWork W2119552130 @default.
- W2561775774 hasRelatedWork W2300403035 @default.
- W2561775774 hasRelatedWork W2318185320 @default.
- W2561775774 hasRelatedWork W2367919734 @default.
- W2561775774 hasRelatedWork W2418024689 @default.
- W2561775774 hasRelatedWork W2611233410 @default.
- W2561775774 hasVolume "62" @default.
- W2561775774 isParatext "false" @default.
- W2561775774 isRetracted "false" @default.
- W2561775774 magId "2561775774" @default.
- W2561775774 workType "article" @default.