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- W2562000147 abstract "fordiagnosing herpetic infections. Fiveofthese conjugates-Microbiological Associates bivalent herpes antiserum(MBA-bivalent), MBA anti-herpes types1(MBA-1)and2(MBA-2), and FlowLaboratories anti-herpes types 1(Flow-i) and2(Flow-2)-were compared forantibody titer, diagnostic accuracy, andnonspecific background staining on knowninfected tissue. Fourofthese weretested simultaneously on75clinical specimens. Noattempt wasmadetodifferentiate between herpes simplex virus type1ortype2infections. Allantisera werecomparable intiter andexhibited fluorescence withbothherpes simplex virus types1and2antigens. Themost significant difference wasexcessive nonspecific staining consistently seenwith theMBA-2conjugate. Overall, Flow-2 andMBA-bivalent antisera werepreferable. Herpes simplex virus (HSV)isamajorcause ofgenital ulcers, vaginitis, andcervicitis. The last twoconditions arerarely diagnosed clinically. Thelabial mucousmembrane ulcers may suggest aherpetic etiology ifpainful, butthey arerarely seenintheclassic vesicular stage and areoften misdiagnosed aschancroid, syphilis, trauma, orevencarcinoma (3). Direct immunofluorescence canrapidly andaccurately differentiate herpesvirus infection (7). However, the timeandexpense ofreagent preparation has limited theuseofthistechnique toresearch laboratories. Inthepresent study, fivedirect fluorescent-antibody conjugates now commercially available-Microbiological bivalent herpes antiserum (MBA-bivalent), Microbiological Associates anti-herpes types1 (MBA-1) and2(MBA-2), andFlowLaboratories anti-herpes types1 (Flow-1) and2 (Flow2)-were compared, using smearsofcells from knowninfected rabbit kidney (RK)tissue cultureand,withtheexception ofFlow-1, cell scrapings of75suspected herpetic genital le" @default.
- W2562000147 created "2017-01-06" @default.
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- W2562000147 date "1977-01-01" @default.
- W2562000147 modified "2023-09-27" @default.
- W2562000147 title "Clinical Evaluation ofCommercial Conjugates forDirect Immunofluorescence" @default.
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